Real Time Differentiation of G-Protein Coupled Receptor (GPCR) Agonist and Antagonist by Two Photon Fluorescence Laser Microscopy

2004 ◽  
Vol 126 (23) ◽  
pp. 7160-7161 ◽  
Author(s):  
Minying Cai ◽  
Magda Stankova ◽  
Stephanie J. K. Pond ◽  
Alexander V. Mayorov ◽  
Joseph W. Perry ◽  
...  
Keyword(s):  
Real Time ◽  
G Protein ◽  
G Protein Coupled Receptor ◽  
Laser Microscopy ◽  
Two Photon ◽  
Agonist And Antagonist ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence ◽  
G Protein Coupled

Cancer cell-targeted two-photon fluorescence probe for the real-time ratiometric imaging of DNA damage

2016 ◽  
Vol 52 (37) ◽  
pp. 6308-6311 ◽  
Author(s):  
Hua Zhang ◽  
Kui Wang ◽  
Xiaopeng Xuan ◽  
Qingzhang Lv ◽  
Yamin Nie ◽  
...  
Keyword(s):  
Dna Damage ◽  
Cancer Cells ◽  
Real Time ◽  
Cancer Cell ◽  
Fluorescence Probe ◽  
The Real ◽  
Two Photon ◽  
Ratiometric Imaging ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

A sequential ICT fluorescence probe (ANF–Glu) was successfully utilized to spatially and temporally image DNA damage in cancer cells.

scholarly journals Real-time pixelwise phasor analysis for videorate two-photon fluorescence lifetime imagingmicroscopy

2021 ◽  
Author(s):  
Janet Sorrells ◽  
Rishyashring Iyer ◽  
Lingxiao Yang ◽  
Andrew Bower ◽  
Darold Spillman ◽  
...  
Keyword(s):  
Real Time ◽  
Fluorescence Lifetime ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence ◽  
Phasor Analysis

scholarly journals Real-Time Detection of Interactions between the Human Oxytocin Receptor and G Protein-Coupled Receptor Kinase-2

2004 ◽  
Vol 18 (5) ◽  
pp. 1277-1286 ◽  
Author(s):  
Ahmed Hasbi ◽  
Dominic Devost ◽  
Stéphane A. Laporte ◽  
Hans H. Zingg
Keyword(s):  
Real Time ◽  
G Protein ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Oxytocin Receptor ◽  
Dominant Negative ◽  
Receptor Kinase ◽  
G Protein Coupled Receptor ◽  
Time Resolved ◽  
G Protein Coupled

Abstract Although the oxytocin receptor (OTR) mediates many important functions including uterine contractions, milk ejection, and maternal behavior, the mechanisms controlling agonist-induced OTR desensitization have remained unclear, and attempts to demonstrate involvement of a G protein-coupled receptor kinase (GRK) have so far failed. Using the OTR as a model, we demonstrate here directly for the first time the dynamics of agonist-induced interactions of a GRK with a G protein-coupled receptor in real time, using time-resolved bioluminescence resonance energy transfer. GRK2/receptor interactions started within 4 sec, peaked at 10 sec, and decreased to less than 40% within 8 min. By contrast, β-arrestin/OTR interactions initiated only at 10 sec, reached plateau levels at 120 sec, but remained stable with little decrease thereafter. Physical GRK2/OTR association was further demonstrated by coimmunoprecipitation of endogenous GRK2 with activated OTR. In COS-7 cells, which express low levels of GRK2 and β-arrestin, overexpression of GRK2 and β-arrestin increased receptor phosphorylation, desensitization, and internalization to the high levels observed in human embryonic kidney 293 cells. By contrast, specific inhibition of endogenous GRK2 by dominant-negative mutants robustly inhibited OTR phosphorylation and internalization as well as arrestin/OTR interactions. These data characterize the temporal and causal relationship of GRK-2/OTR and β-arrestin/OTR interactions and establish GRK/OTR interaction as a prerequisite for β-arrestin-mediated OTR desensitization.

scholarly journals Single molecule binding of a ligand to a G-protein-coupled receptor in real time using fluorescence correlation spectroscopy, rendered possible by nano-encapsulation in styrene maleic acid lipid particles

Nanoscale ◽  
2020 ◽  
Vol 12 (21) ◽  
pp. 11518-11525 ◽  
Author(s):  
Rachael L. Grime ◽  
Joelle Goulding ◽  
Romez Uddin ◽  
Leigh A. Stoddart ◽  
Stephen J. Hill ◽  
...  
Keyword(s):  
Real Time ◽  
G Protein ◽  
Single Molecule ◽  
Maleic Acid ◽  
Fluorescence Correlation Spectroscopy ◽  
Correlation Spectroscopy ◽  
G Protein Coupled Receptor ◽  
Fluorescence Correlation ◽  
Lipid Particles ◽  
G Protein Coupled

Combining the technologies of encapsulation of GPCRs in SMA lipid particles with fluorescence correlation spectroscopy provides a versatile characterisation platform.

A two-photon fluorescence self-reporting black phosphorus nanoprobe for the in situ monitoring of therapy response

2020 ◽  
Vol 56 (90) ◽  
pp. 14007-14010
Author(s):  
Kesong Guan ◽  
Peng Wang ◽  
Fang Zhou ◽  
Youjuan Wang ◽  
Hong-Wen Liu ◽  
...  
Keyword(s):  
Real Time ◽  
Therapeutic Response ◽  
Therapy Response ◽  
Black Phosphorus ◽  
In Situ Monitoring ◽  
Two Photon ◽  
Fluorescent Nanoprobe ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

We developed a black phosphorus based two-photon fluorescent nanoprobe (TPBP) for the in situ and real-time reporting of the therapeutic response of black phosphorus.

Sign in / Sign up

Export Citation Format

Share Document