scholarly journals Paranemic Crossover DNA:  A Generalized Holliday Structure with Applications in Nanotechnology

2004 ◽  
Vol 126 (6) ◽  
pp. 1666-1674 ◽  
Author(s):  
Zhiyong Shen ◽  
Hao Yan ◽  
Tong Wang ◽  
Nadrian C. Seeman
Keyword(s):  
Paranemic Crossover

Paranemic Crossover DNA: There and Back Again

2018 ◽  
Author(s):  
Xing Wang ◽  
Arun Richard Chandrasekaran ◽  
Zhiyong Shen ◽  
Yoel P. Ohayon ◽  
Tong Wang ◽  
...  
Keyword(s):  
Paranemic Crossover

Facilitation of DNA self-assembly by relieving the torsional strains between building blocks

2017 ◽  
Vol 15 (2) ◽  
pp. 465-469 ◽  
Author(s):  
Weili Shen ◽  
Qing Liu ◽  
Baoquan Ding ◽  
Changqing Zhu ◽  
Zhiyong Shen ◽  
...  
Keyword(s):  
Self Assembly ◽  
Building Blocks ◽  
Base Pairs ◽  
Dna Motifs ◽  
Torsional Strain ◽  
High Uniformity ◽  
Self Assembled ◽  
Paranemic Crossover

Overwound and underwound paranemic crossover (PX) DNA motifs have been self-assembled to generate high uniformity lattices, by adjusting the number of base pairs in the JX region adjacent to the PX structure to relieve torsional strain.

scholarly journals Structural Rigidity of Paranemic Crossover and Juxtapose DNA Nanostructures

2011 ◽  
Vol 101 (6) ◽  
pp. 1393-1402 ◽  
Author(s):  
Mogurampelly Santosh ◽  
Prabal K. Maiti
Keyword(s):  
Dna Nanostructures ◽  
Structural Rigidity ◽  
Paranemic Crossover

scholarly journals Exceptional Nuclease Resistance of Paranemic Crossover (PX) DNA and Crossover-Dependent Biostability of DNA Motifs

2020 ◽  
Vol 142 (14) ◽  
pp. 6814-6821 ◽  
Author(s):  
Arun Richard Chandrasekaran ◽  
Javier Vilcapoma ◽  
Paromita Dey ◽  
Siu Wah Wong-Deyrup ◽  
Bijan K. Dey ◽  
...  
Keyword(s):  
Dna Motifs ◽  
Nuclease Resistance ◽  
Paranemic Crossover

scholarly journals Atomic-Level Simulations of Seeman DNA Nanostructures: The Paranemic Crossover in Salt Solution

2006 ◽  
Vol 90 (5) ◽  
pp. 1463-1479 ◽  
Author(s):  
Prabal K. Maiti ◽  
Tod A. Pascal ◽  
Nagarajan Vaidehi ◽  
Jiyoung Heo ◽  
William A. Goddard
Keyword(s):  
Salt Solution ◽  
Atomic Level ◽  
Dna Nanostructures ◽  
Paranemic Crossover

The study of the paranemic crossover (PX) motif in the context of self-assembly of DNA 2D crystals

2016 ◽  
Vol 14 (30) ◽  
pp. 7187-7190 ◽  
Author(s):  
Weili Shen ◽  
Qing Liu ◽  
Baoquan Ding ◽  
Zhiyong Shen ◽  
Changqing Zhu ◽  
...  
Keyword(s):  
Optimal Design ◽  
Self Assembly ◽  
Building Blocks ◽  
2D Arrays ◽  
Paranemic Crossover ◽  
2D Crystals

The DNA paranemic crossover (PX) motif has been examined as building blocks for construction of DNA 2D arrays and the optimal design has been discovered.

scholarly journals Exceptional biostability of paranemic crossover (PX) DNA, crossover-dependent nuclease resistance, and implications for DNA nanotechnology

2019 ◽  
Author(s):  
Arun Richard Chandrasekaran ◽  
Javier Vilcapoma ◽  
Paromita Dey ◽  
SiuWah Wong-Deyrup ◽  
Bijan K. Dey ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
Biological Fluids ◽  
Dna Nanotechnology ◽  
Full Potential ◽  
Dna Nanostructures ◽  
Double Stranded Dna ◽  
Nuclease Resistance ◽  
Order Of Magnitude ◽  
Paranemic Crossover ◽  
The Many

AbstractInherent nanometer-sized features and molecular recognition properties make DNA a useful material in constructing nanoscale objects, with alluring applications in biosensing and drug delivery. However, DNA can be easily degraded by nucleases present in biological fluids, posing a considerable roadblock to realizing the full potential of DNA nanotechnology for biomedical applications. Here we investigated the nuclease resistance and biostability of the multi-stranded motif called paranemic crossover (PX) DNA and discovered a remarkable and previously unreported resistance to nucleases. We show that PX DNA has more than an order of magnitude increased resistance to degradation by DNase I, serum, and urine compared to double stranded DNA. We further demonstrate that the degradation resistance decreases monotonically as DNA crossovers are removed from the structure, suggesting that frequent DNA crossovers disrupt either the binding or catalysis of nucleases or both. Further, we show using mouse and human cell lines that PX DNA does not affect cell proliferation or interfere with biological processes such as myogenesis. These results have important implications for building DNA nanostructures with enhanced biostability, either by adopting PX-based architectures or by carefully engineering crossovers. We contend that such crossover-dependent nuclease resistance could potentially be used to add “tunable biostability” to the many features of DNA nanotechnology.

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