Efficient Delivery of Streptavidin to Mammalian Cells:  Clathrin-Mediated Endocytosis Regulated by a Synthetic Ligand

2002 ◽  
Vol 124 (22) ◽  
pp. 6265-6273 ◽  
Author(s):  
Stephen L. Hussey ◽  
Blake R. Peterson
Keyword(s):  
Mammalian Cells ◽  
Efficient Delivery ◽  
Synthetic Ligand

scholarly journals Protein Transduction Domain of HIV-1 Tat Protein Promotes Efficient Delivery of DNA into Mammalian Cells

2001 ◽  
Vol 276 (28) ◽  
pp. 26204-26210 ◽  
Author(s):  
Akiko Eguchi ◽  
Teruo Akuta ◽  
Hajime Okuyama ◽  
Takao Senda ◽  
Haruhiko Yokoi ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Protein Transduction ◽  
Protein Transduction Domain ◽  
Tat Protein ◽  
Efficient Delivery ◽  
Hiv 1

scholarly journals Histidine-rich amphipathic peptide antibiotics promote efficient delivery of DNA into mammalian cells

2003 ◽  
Vol 100 (4) ◽  
pp. 1564-1568 ◽  
Author(s):  
A. Kichler ◽  
C. Leborgne ◽  
J. Marz ◽  
O. Danos ◽  
B. Bechinger
Keyword(s):  
Mammalian Cells ◽  
Peptide Antibiotics ◽  
Amphipathic Peptide ◽  
Efficient Delivery

scholarly journals Vertical silicon nanowires as a universal platform for delivering biomolecules into living cells

2010 ◽  
Vol 107 (5) ◽  
pp. 1870-1875 ◽  
Author(s):  
Alex K. Shalek ◽  
Jacob T. Robinson ◽  
Ethan S. Karp ◽  
Jin Seok Lee ◽  
Dae-Ro Ahn ◽  
...  
Keyword(s):  
Small Molecules ◽  
High Throughput ◽  
Silicon Nanowires ◽  
Mammalian Cells ◽  
Living Cells ◽  
Diverse Range ◽  
Viral Packaging ◽  
Neuronal Progenitor ◽  
Efficient Delivery ◽  
Surface Modified

A generalized platform for introducing a diverse range of biomolecules into living cells in high-throughput could transform how complex cellular processes are probed and analyzed. Here, we demonstrate spatially localized, efficient, and universal delivery of biomolecules into immortalized and primary mammalian cells using surface-modified vertical silicon nanowires. The method relies on the ability of the silicon nanowires to penetrate a cell’s membrane and subsequently release surface-bound molecules directly into the cell’s cytosol, thus allowing highly efficient delivery of biomolecules without chemical modification or viral packaging. This modality enables one to assess the phenotypic consequences of introducing a broad range of biological effectors (DNAs, RNAs, peptides, proteins, and small molecules) into almost any cell type. We show that this platform can be used to guide neuronal progenitor growth with small molecules, knock down transcript levels by delivering siRNAs, inhibit apoptosis using peptides, and introduce targeted proteins to specific organelles. We further demonstrate codelivery of siRNAs and proteins on a single substrate in a microarray format, highlighting this technology’s potential as a robust, monolithic platform for high-throughput, miniaturized bioassays.

scholarly journals MultiBac: Baculovirus-Mediated Multigene DNA Cargo Delivery in Insect and Mammalian Cells

Viruses ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 198 ◽  
Author(s):  
Kapil Gupta ◽  
Christine Tölzer ◽  
Duygu Sari-Ak ◽  
Daniel Fitzgerald ◽  
Christiane Schaffitzel ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Viral Vectors ◽  
Genome Engineering ◽  
Protein Complexes ◽  
Cell System ◽  
Heterologous Protein Expression ◽  
Synthetic Dna ◽  
Cargo Delivery ◽  
Efficient Delivery ◽  
Recent Developments

The baculovirus/insect cell system (BICS) is widely used in academia and industry to produce eukaryotic proteins for many applications, ranging from structure analysis to drug screening and the provision of protein biologics and therapeutics. Multi-protein complexes have emerged as vital catalysts of cellular function. In order to unlock the structure and mechanism of these essential molecular machines and decipher their function, we developed MultiBac, a BICS particularly tailored for heterologous multigene transfer and multi-protein complex production. Baculovirus is unique among common viral vectors in its capacity to accommodate very large quantities of heterologous DNA and to faithfully deliver this cargo to a host cell of choice. We exploited this beneficial feature to outfit insect cells with synthetic DNA circuitry conferring new functionality during heterologous protein expression, and developing customized MultiBac baculovirus variants in the process. By altering its tropism, recombinant baculovirions can be used for the highly efficient delivery of a customized DNA cargo in mammalian cells and tissues. Current advances in synthetic biology greatly facilitate the construction or recombinant baculoviral genomes for gene editing and genome engineering, mediated by a MultiBac baculovirus tailored to this purpose. Here, recent developments and exploits of the MultiBac system are presented and discussed.

Efficient Delivery of Antisense Oligonucleotides by an Amphipathic Cell-Penetrating Peptide in Acinetobacter baumannii

2019 ◽  
Vol 16 (8) ◽  
pp. 728-736 ◽  
Author(s):  
Zhou Chen ◽  
Dan Nie ◽  
Yue Hu ◽  
Mingkai Li ◽  
Zheng Hou ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Antisense Oligonucleotides ◽  
Mammalian Cells ◽  
Acyl Carrier Protein ◽  
High Specificity ◽  
Independent Manner ◽  
Amphipathic Peptide ◽  
Carbapenem Resistant ◽  
Efficient Delivery ◽  
Novel Strategy

Background: Carbapenem-resistant Acinetobacter baumannii (A. baumannii) was on the top of the list of the most threatening bacteria published by the WHO in 2017. Antisense oligonucleotides (ASOs) based therapy is a promising strategy for combating Multi-Drug Resistant (MDR) bacteria because of its high specificity, easy design and lower induction of resistance, but poor cellular uptake by bacteria has restricted the further utilization of this therapy. Methods: Here, we used CADY, a secondary amphipathic peptide of 20 residues that could successfully carry siRNA into mammalian cells, to prepare CADY/ASOs nanoparticles (CADY-NPs) targeting acpP (encoding acyl carrier protein), and evaluated the uptake features, the inhibitory effects of CADY-NPs on gene expression and the growth of MDR-A. baumannii. Results: We found that CADY-NPs could be quickly internalized by drug-sensitive and MDR-A. baumannii in an energy independent manner, which could be restrained by chlorpromazine (an inhibitor of clathrin mediated endocytosis) significantly. In addition, CADY-NPs targeting acpP concentrationdependently retarded the growth of MDR-A. baumannii, which was associated with the decreased expression of targeted genes in A. baumannii. Conclusion: In conclusion, our research is the first to demonstrate that CADY can deliver ASOs into bacteria and provide a novel strategy for the treatment of MDR-A. baumannii.

scholarly journals Optimized Silica-Binding Peptide-Mediated Delivery of Bactericidal Lysin Efficiently Prevents Staphylococcus aureus from Adhering to Device Surfaces

2021 ◽  
Vol 22 (22) ◽  
pp. 12544
Author(s):  
Wan Yang ◽  
Vijay Singh Gondil ◽  
Dehua Luo ◽  
Jin He ◽  
Hongping Wei ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Mammalian Cells ◽  
Bacterial Colonization ◽  
Antibiotic Resistant ◽  
Binding Peptide ◽  
Long Term Stability ◽  
Efficient Delivery ◽  
Generic Strategy ◽  
Recycling Potential ◽  
Functionalized Coatings

Staphylococcal-associated device-related infections (DRIs) represent a significant clinical challenge causing major medical and economic sequelae. Bacterial colonization, proliferation, and biofilm formation after adherence to surfaces of the indwelling device are probably the primary cause of DRIs. To address this issue, we incorporated constructs of silica-binding peptide (SiBP) with ClyF, an anti-staphylococcal lysin, into functionalized coatings to impart bactericidal activity against planktonic and sessile Staphylococcus aureus. An optimized construct, SiBP1-ClyF, exhibited improved thermostability and staphylolytic activity compared to its parental lysin ClyF. SiBP1-ClyF-functionalized coatings were efficient in killing MRSA strain N315 (>99.999% within 1 h) and preventing the growth of static and dynamic S. aureus biofilms on various surfaces, including siliconized glass, silicone-coated latex catheter, and silicone catheter. Additionally, SiBP1-ClyF-immobilized surfaces supported normal attachment and growth of mammalian cells. Although the recycling potential and long-term stability of lysin-immobilized surfaces are still affected by the fragility of biological protein molecules, the present study provides a generic strategy for efficient delivery of bactericidal lysin to solid surfaces, which serves as a new approach to prevent the growth of antibiotic-resistant microorganisms on surfaces in hospital settings and could be adapted for other target pathogens as well.

A Cationic Lipid for Rapid and Efficient Delivery of Plasmid DNA into Mammalian Cells

1997 ◽  
Vol 236 (1) ◽  
pp. 126-129 ◽  
Author(s):  
Guohong Zhang ◽  
Vanessa Gurtu ◽  
Thomas H. Smith ◽  
Paul Nelson ◽  
Steven R. Kain
Keyword(s):  
Plasmid Dna ◽  
Mammalian Cells ◽  
Cationic Lipid ◽  
Efficient Delivery

scholarly journals Efficient Delivery of Cyclic Peptides into Mammalian Cells with Short Sequence Motifs

2012 ◽  
Vol 8 (2) ◽  
pp. 423-431 ◽  
Author(s):  
Ziqing Qian ◽  
Tao Liu ◽  
Yu-Yu Liu ◽  
Roger Briesewitz ◽  
Amy M. Barrios ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Cyclic Peptides ◽  
Sequence Motifs ◽  
Short Sequence ◽  
Efficient Delivery
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