A Cationic Lipid for Rapid and Efficient Delivery of Plasmid DNA into Mammalian Cells

1997 ◽  
Vol 236 (1) ◽  
pp. 126-129 ◽  
Author(s):  
Guohong Zhang ◽  
Vanessa Gurtu ◽  
Thomas H. Smith ◽  
Paul Nelson ◽  
Steven R. Kain
Keyword(s):  
Plasmid Dna ◽  
Mammalian Cells ◽  
Cationic Lipid ◽  
Efficient Delivery

Arf6-mediated macropinocytosis enhanced suicide gene therapy of C16TAB-condensed Tat/pDNA nanoparticles in ovarian cancer

Nanoscale ◽  
2021 ◽  
Author(s):  
Zhe Sun ◽  
Jinhai Huang ◽  
Linjia Su ◽  
Jing Li ◽  
Fangzheng Qi ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Gene Therapy ◽  
Cancer Treatment ◽  
Plasmid Dna ◽  
Suicide Gene ◽  
Cell Penetrating Peptides ◽  
Therapeutic Gene ◽  
Hiv Tat ◽  
Efficient Delivery ◽  
Cell Penetrating

Using cell-penetrating peptides (CPPs), typically HIV-Tat, to deliver the therapeutic gene for cancer treatment has being hampered by low efficient delivery and complicated uptake route of plasmid DNA (pDNA). On...

Assembly of Plasmid DNA into Liposomes After Condensation by Cationic Lipid in Anionic Detergent Solution

2005 ◽  
Vol 27 (21) ◽  
pp. 1701-1705 ◽  
Author(s):  
Hong-Wei Zhang ◽  
Ling Zhang ◽  
Xun Sun ◽  
Shu Diao ◽  
Zhi-Rong Zhang
Keyword(s):  
Plasmid Dna ◽  
Cationic Lipid ◽  
Detergent Solution

scholarly journals Protein Transduction Domain of HIV-1 Tat Protein Promotes Efficient Delivery of DNA into Mammalian Cells

2001 ◽  
Vol 276 (28) ◽  
pp. 26204-26210 ◽  
Author(s):  
Akiko Eguchi ◽  
Teruo Akuta ◽  
Hajime Okuyama ◽  
Takao Senda ◽  
Haruhiko Yokoi ◽  
...  
Keyword(s):  
Mammalian Cells ◽  
Protein Transduction ◽  
Protein Transduction Domain ◽  
Tat Protein ◽  
Efficient Delivery ◽  
Hiv 1

scholarly journals Influence of Environmental Conditions on the Fusion of Cationic Liposomes with Living Mammalian Cells

Nanomaterials ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 1025 ◽  
Author(s):  
Rejhana Kolašinac ◽  
Sebastian Jaksch ◽  
Georg Dreissen ◽  
Andrea Braeutigam ◽  
Rudolf Merkel ◽  
...  
Keyword(s):  
Lipid Membranes ◽  
Mammalian Cells ◽  
High Efficiency ◽  
Phase State ◽  
Chinese Hamster ◽  
Cationic Lipid ◽  
Cationic Liposomes ◽  
Biological Macromolecules ◽  
Lipid Mixture ◽  
Fusion Efficiency

Lipid-based nanoparticles, also called vesicles or liposomes, can be used as carriers for drugs or many types of biological macromolecules, including DNA and proteins. Efficiency and speed of cargo delivery are especially high for carrier vesicles that fuse with the cellular plasma membrane. This occurs for lipid mixture containing equal amounts of the cationic lipid DOTAP and a neutral lipid with an additional few percents of an aromatic substance. The fusion ability of such particles depends on lipid composition with phosphoethanolamine (PE) lipids favoring fusion and phosphatidyl-choline (PC) lipids endocytosis. Here, we examined the effects of temperature, ionic strength, osmolality, and pH on fusion efficiency of cationic liposomes with Chinese hamster ovary (CHO) cells. The phase state of liposomes was analyzed by small angle neutron scattering (SANS). Our results showed that PC containing lipid membranes were organized in the lamellar phase. Here, fusion efficiency depended on buffer conditions and remained vanishingly small at physiological conditions. In contrast, SANS indicated the coexistence of very small (~50 nm) objects with larger, most likely lamellar structures for PE containing lipid particles. The fusion of such particles to cell membranes occurred with very high efficiency at all buffer conditions. We hypothesize that the altered phase state resulted in a highly reduced energetic barrier against fusion.

Transfection of plasmid DNA by nanocarriers containing a gemini cationic lipid with an aromatic spacer or its monomeric counterpart

2018 ◽  
Vol 161 ◽  
pp. 519-527 ◽  
Author(s):  
María Martínez-Negro ◽  
Ana L. Barrán-Berdón ◽  
Clara Aicart-Ramos ◽  
María L. Moyá ◽  
Conchita Tros de Ilarduya ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Cationic Lipid

Hamster polyomavirus-derived virus-like particles are able to transfer in vitro encapsidated plasmid DNA to mammalian cells

Virus Genes ◽  
2007 ◽  
Vol 34 (3) ◽  
pp. 303-314 ◽  
Author(s):  
Tatyana Voronkova ◽  
Andris Kazaks ◽  
Velta Ose ◽  
Muhsin Özel ◽  
Siegfried Scherneck ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Mammalian Cells ◽  
Virus Like Particles ◽  
Hamster Polyomavirus

scholarly journals Cellular gene transfer mediated by influenza virosomes with encapsulated plasmid DNA

2007 ◽  
Vol 405 (1) ◽  
pp. 41-49 ◽  
Author(s):  
Jørgen de Jonge ◽  
Johanna M. Leenhouts ◽  
Marijke Holtrop ◽  
Pieter Schoen ◽  
Peter Scherrer ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Gradient Centrifugation ◽  
Cationic Lipid ◽  
Sucrose Density Gradient ◽  
Target Cells ◽  
Sucrose Density Gradient Centrifugation ◽  
Viral Membrane ◽  
Viral Envelopes

Reconstituted influenza virosomes (virus membrane envelopes) have been used previously to deliver pDNA (plasmid DNA) bound to their external surface to a variety of target cells. Although high transfection efficiencies can be obtained with these complexes in vitro, the virosome-associated DNA is readily accessible to nucleases and could therefore be prone to rapid degradation under in vivo conditions. In the present study, we show a new method for the production of DNA–virosomes resulting in complete protection of the DNA from nucleases. This method relies on the use of the short-chain phospholipid DCPC (dicaproylphosphatidylcholine) for solubilization of the viral membrane. The solubilized viral membrane components are mixed with pDNA and cationic lipid. Reconstitution of the viral envelopes and simultaneous encapsulation of pDNA is achieved by removal of the DCPC from the mixture through dialysis. Analysis by linear sucrose density-gradient centrifugation revealed that protein, phospholipid and pDNA physically associated to particles, which appeared as vesicles with spike proteins inserted in their membranes when analysed by electron microscopy. The DNA–virosomes retained the membrane fusion properties of the native influenza virus. The virosome-associated pDNA was completely protected from degradation by nucleases, providing evidence for the DNA being highly condensed and encapsulated in the lumen of the virosomes. DNA–virosomes, containing reporter gene constructs, transfected a variety of cell lines, with efficiencies approaching 90%. Transfection was completely dependent on the fusogenic properties of the viral spike protein haemagglutinin. Thus, DNA–virosomes prepared by the new procedure are highly efficient vehicles for DNA delivery, offering the advantage of complete DNA protection, which is especially important for future in vivo applications.

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