Incorporation of Isotopic Formate into the Thymine of Bone Marrow Deoxyribonucleic Acid in Vitro1

1954 ◽  
Vol 76 (8) ◽  
pp. 2196-2197 ◽  
Author(s):  
John R. Totter
Keyword(s):  
Bone Marrow ◽  
Deoxyribonucleic Acid

Peptide synthesis in bone marrow: insulin and thyroxin effects

1962 ◽  
Vol 203 (4) ◽  
pp. 693-696 ◽  
Author(s):  
Thomas F. Necheles
Keyword(s):  
Bone Marrow ◽  
Nucleic Acid ◽  
Peptide Synthesis ◽  
Ribonucleic Acid ◽  
Deoxyribonucleic Acid ◽  
Optimum Concentration ◽  
The Other ◽  
Anabolic Effect ◽  
Oxygen Buffer

Myeloid marrow was rapidly removed from femurs of fasting young rabbits, sectioned, and incubated in Krebs-bicarbonate-CO2-oxygen buffer with appropriate C14-labeled precursors. All manipulations were designed to preserve the architecture of the tissue. After 1 hr the protein or nucleic acid-adenine was isolated and purified. Insulin, 0.01 U/ml added in vitro, stimulated histidine-2(ring)-C14 incorporation into protein by 26 ± 1.4%; alkali-treated insulin was inactive. Thyroxin elicited a 49.4 ± 2.1% stimulation at an optimum concentration of 10–7 m. Triiodothyronine, but not diiodothyronine, also had a significant effect. Insulin increased incorporation of carbon from adenosine-8-C14 into adenine of ribonucleic acid and deoxyribonucleic acid. Thyroxin, on the other hand, was without consistent effect on this process. Thyroxin stimulated significantly the incorporation of C14 of glycine-2-C14 into adenine. The possibility that part of the anabolic effect of thyroxin on bone marrow may arise from a stimulus to incorporation of precursors into purines is suggested.

Influence of Post-Irradiation Administration of Methionine on the Levels of Deoxyribonucleic Acid in Bone Marrow, Spleen and Liver of Rats

Nature ◽  
1957 ◽  
Vol 180 (4578) ◽  
pp. 193-194 ◽  
Author(s):  
M. K. NERURKAR ◽  
A. J. BAXI ◽  
N. S. RANADIVE ◽  
M. V. NARURKAR ◽  
M. B. SAHASRABUDHE
Keyword(s):  
Bone Marrow ◽  
Deoxyribonucleic Acid ◽  
Post Irradiation

Cellularity and Deoxyribonucleic Acid Synthesis in Bone Marrow after Total- and Partial-Body Irradiation

1961 ◽  
Vol 14 (5) ◽  
pp. 618 ◽  
Author(s):  
A. Tsuya ◽  
V. P. Bond ◽  
T. M. Fliedner ◽  
L. E. Feinendegen
Keyword(s):  
Bone Marrow ◽  
Deoxyribonucleic Acid ◽  
Acid Synthesis ◽  
Deoxyribonucleic Acid Synthesis ◽  
Partial Body
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