Formation of Different Hæmoglobins in Tissue Culture of Human Bone Marrow Treated with Human Deoxyribonucleic Acid

Nature ◽  
1961 ◽  
Vol 192 (4807) ◽  
pp. 1055-1057 ◽  
Author(s):  
LORRAINE M. KRAUS
Keyword(s):  
Bone Marrow ◽  
Tissue Culture ◽  
Deoxyribonucleic Acid ◽  
Human Bone ◽  
Human Bone Marrow

Tissue culture surface characteristics influence the expansion of human bone marrow cells

Biomaterials ◽  
1998 ◽  
Vol 19 (21) ◽  
pp. 1963-1972 ◽  
Author(s):  
Manfred R. Koller ◽  
Mahshid A. Palsson ◽  
Ilana Manchel ◽  
Robert J. Maher ◽  
Bernhard Ø. Palsson
Keyword(s):  
Bone Marrow ◽  
Tissue Culture ◽  
Bone Marrow Cells ◽  
Surface Characteristics ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Culture Surface ◽  
Marrow Cells

scholarly journals Motility Studies of Human Postmortem Bone Marrow

Blood ◽  
1960 ◽  
Vol 16 (1) ◽  
pp. 1020-1028 ◽  
Author(s):  
VERNON P. PERRY ◽  
ROBERT E. STEVENSON ◽  
WILLIAM MCFARLAND ◽  
GEORGE A. ZINK
Keyword(s):  
Bone Marrow ◽  
Tissue Culture ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Human Cadaver ◽  
Adequate Supply ◽  
Motile Cells ◽  
Marrow Cells

Abstract The source of an adequate supply of human bone marrow for transplantation purposes presents a distinct problem. The use of cadaver marrow has been suggested. Studies of 70 human cadaver bone marrow preparations obtained from 1.5 to over 102 hours postmortem were examined by tissue culture and photomicrographic technics to correlate the survival of leukocyte motility with the time postmortem. Motile cells were present in marrow up to and including 50 hours after death, thus suggesting the possibility of using such marrow as a practical source of viable marrow cells.

Tissue culture studies of human bone marrow

1969 ◽  
Vol 54 (1) ◽  
pp. 53-57 ◽  
Author(s):  
Patricia Farnes ◽  
Barbara E. Barker
Keyword(s):  
Bone Marrow ◽  
Tissue Culture ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Culture Studies

scholarly journals Long-Term Tissue Culture of Human Bone Marrow

Blood ◽  
1955 ◽  
Vol 10 (9) ◽  
pp. 896-911 ◽  
Author(s):  
LAWRENCE BERMAN ◽  
CYRIL S. STULBERG ◽  
FRANK H. RUDDLE ◽  
NANCY CUNNINGHAM
Keyword(s):  
Bone Marrow ◽  
Tissue Culture ◽  
Epithelial Cells ◽  
Continuous Cultivation ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Morphologic Characteristics ◽  
Pass Through ◽  
Hela Strain

Abstract Using methods described, long-term tissue cultures of human bone marrow pass through three typical growth phases : (1) a myeloid phase during which myeloid cells can be recognized; (2) a round-cell phase during which histioid and monocytoid cells predominate; and (3) a fibroblastic phase which can be maintained thereafter. The three phases are of variable onset and duration. In a culture from a patient with carcinoma of the lung, isolated colonies of polygonal cells appeared on the 51st day of continuous cultivation. These were removed and subcultured. By means of trypsinization, the sheets of polygonal cells were transferred and subcultured in bulk and in a manner making it possible to obtain replicate cultures. The morphologic characteristics and growth behavior of the strain of cells (Detroit-6 strain) are similar to those observed for HeLa cells. The pattern of growth of the Detroit-6 strain is that of epithelial cells, presumably of metastatic origin from a carcinoma of the lung. The susceptibilities of the Detroit-6 strain to infection with various viruses (poliomyelitis, Coxsackie, herpes simplex, and influenza) are similar to those observed in the HeLa strain of human malignant epithelial cells originating from a carcinoma of the cervix. The possible values of long-term tissue culture of human bone marrow have been discussed.

scholarly journals Tissue Culture of Bone Marrow

Blood ◽  
1966 ◽  
Vol 27 (4) ◽  
pp. 460-469 ◽  
Author(s):  
EDWARD H. REISNER
Keyword(s):  
Bone Marrow ◽  
Tissue Culture ◽  
Salt Solution ◽  
Horse Serum ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Normal Human ◽  
Blood Elements ◽  
Normal Human Bone Marrow

Abstract In cultures of normal human bone marrow in a medium of 20 per cent horse serum and balanced salt solution, estrone stimulated granulopoiesis; testosterone inhibited fibroblast formation markedly and stimulated erythropoiesis; prednisolone inhibited fibroblast formation completely and allowed continued differentiation of all blood elements in smaller numbers.

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