scholarly journals THE CLARIFICATION OF SOLUTIONS CONTAINING REDUCING SUGARS BY BASIC LEAD ACETATE. THE EFFECT OF DIFFERENT DELEADING AGENTS

1922 ◽  
Vol 44 (4) ◽  
pp. 865-867 ◽  
Author(s):  
Duane T. Englis ◽  
Chuk Yee Tsang
Keyword(s):  
Lead Acetate ◽  
Reducing Sugars

Paper ionophoresis of carbohydrates. I. Procedures and results for four electrolytes

1959 ◽  
Vol 12 (1) ◽  
pp. 65 ◽  
Author(s):  
JL Frahn ◽  
JA Mills
Keyword(s):  
Sodium Hydroxide ◽  
Lead Acetate ◽  
Sodium Arsenite ◽  
Reducing Sugars ◽  
Sugar Alcohols ◽  
Polyhydroxy Compounds ◽  
Hexose Sugars ◽  
The Common ◽  
Derivatives Of

Details are given of procedures for effecting paper ionophoresis of polyhydroxy compounds in the electrolytes borax, sodium arsenite, basic lead acetate, and sodium hydroxide. and for detecting the compounds after ionophoresis. Rates of migration are reported for 96 compounds, including all pentose and hexose sugars, the common disaccharides, all sugar alcohols up to the heptitols, the cyclitols, a number of glycols, and several glycosides and other derivatives of carbohydrates. Some new or improved reagents have been developed for locating carbohydrates on paper strips under various conditions. Sodium arsenite and basic lead acetate are the most effective electrolytes for separating reducing sugars, basic lead acetate is the best for separating sugar alcohols, and borax is the best for simple glycols. Some success has been achieved in correlating the configurations of stereoisomers with their mobilities in paper ionophoresis.

scholarly journals The estimation of carbohydrates. V. The supposed precipitation of reducing sugars by basic lead acetate

1916 ◽  
Vol 8 (1) ◽  
pp. 7-15 ◽  
Author(s):  
William A. Davis
Keyword(s):  
Lead Acetate ◽  
Reducing Sugars ◽  
Rotatory Power ◽  
Invert Sugar ◽  
Lead Compound ◽  
Sugar Analysis ◽  
Negative Reading ◽  
The Subject ◽  
Source Of Error

Gill in 1871 first pointed out that when an excess of basic lead acetate is added to a solution of invert sugar the negative rotation of the latter is greatly reduced owing to the formation of a soluble lead compound of laevulose. If sufficient lead solution is added the negative rotation may become a positive one; thus in one experiment quoted by Gill a negative reading of – 28° was transformed into a positive value of + 57°. The change of rotation was not, however, permanent and on removing the lead or on acidifying the solution the original rotatory power was restored. The change of rotation was attributed to an effect of the lead on the laevulose only; a solution of dextrose was practically unaffected by the presence of basic lead acetate. Since Gill's paper the effect of basic lead acetate as a source of error in sugar analysis has been the subject of numerous papers especially by Pellet, Svoboda, Edson, Prinsen Geerligs, Watts and Tempany and Eynon.

DEGRADATION OF D-GLUCOSE-1-C14 TO TRIOSE-REDUCTONE-C14

1955 ◽  
Vol 33 (12) ◽  
pp. 1824-1828 ◽  
Author(s):  
H. F. Bauer ◽  
Carol Teed
Keyword(s):  
Sodium Hydroxide ◽  
Lead Acetate ◽  
Reducing Sugars ◽  
Elevated Temperatures ◽  
Formation Mechanisms ◽  
Glucose Molecule ◽  
Glycerol Formation

Triose-reductone-C14 was obtained by treating D-glucose-1-C14 with sodium hydroxide in the presence of lead acetate at elevated temperatures. Carbon atoms four, five, and six, as well as carbon atoms one, two, and three, of the D-glucose molecule are shown to contribute to the triose-reductone yield. The formation of triose-reductone was found not to be accompanied by glycerol formation. Mechanisms for fragmentation of reducing sugars are discussed in the light of these findings.

Plasmalemma localisation of inorganic phosphate in B cells pancreas

1978 ◽  
Vol 36 (2) ◽  
pp. 528-529
Author(s):  
F. B. P. Wooding ◽  
K. Pedley ◽  
N. Freinkel ◽  
R. M. C. Dawson
Keyword(s):  
Electron Microscope ◽  
B Cells ◽  
B Cell ◽  
Pancreatic Islets ◽  
High Glucose ◽  
Lead Acetate ◽  
Inorganic Phosphate ◽  
Slight Modification ◽  
Uranyl Acetate ◽  
Lead Phosphate

Freinkel et al (1974) demonstrated that isolated perifused rat pancreatic islets reproduceably release up to 50% of their total inorganic phosphate when the concentration of glucose in the perifusion medium is raised.Using a slight modification of the Libanati and Tandler (1969) method for localising inorganic phosphate by fixation-precipitation with glutaraldehyde-lead acetate we can demonstrate there is a significant deposition of lead phosphate (identified by energy dispersive electron microscope microanalysis) at or on the plasmalemma of the B cell of the islets (Fig 1, 3). Islets after incubation in high glucose show very little precipitate at this or any other site (Fig 2). At higher magnification the precipitate seems to be intracellular (Fig 4) but since any use of osmium or uranyl acetate to increase membrane contrast removes the precipitate of lead phosphate it has not been possible to verify this as yet.

A complex network of “snake-like tubules” revealed by the NADPase cytochemical reaction in the acinar cell of pancreas

1984 ◽  
Vol 42 ◽  
pp. 666-667
Author(s):  
A.R. Beaudoin ◽  
G. Grondin ◽  
A. Lord ◽  
M. Pelletier
Keyword(s):  
Acinar Cell ◽  
Lead Acetate ◽  
Ultrastructural Localization ◽  
Sodium Acetate Buffer ◽  
Zymogen Granules ◽  
Sprague Dawley ◽  
Dense Bodies ◽  
Sprague Dawley Rats ◽  
Cytochemical Reaction ◽  
Cellular Organelles

We have recently described the ultrastructural localization of NADPase activity in the exocrine pancreas of rat. The enzyme was found in the intermediate saccules of the Golgi apparatus, in dense bodies and lysosomes but was absent from zymogen granules. A very intense reaction was noticed in a peculiar structure which was termed “Snake-Like Tubule” (SLT). The purposes of the present study were firstly to delineate SLT distribution in the acinar cell and secondly to define any possible relationship or association with other cellular organelles.NADPase cytochemical reaction was performed on the pancreas of adult Sprague Dawley rats. Small lobules were excised and fixed for 50 min, at 4°C, in 2% glutaraldehyde buffered with 0.1M cacodylate at pH 7.2. Lobules were rinsed several times with the same buffer containing 570 sucrose and cut with a Mcllwayn tissue chopper. Sections were washed several times with buffer and incubated for 2 hr at 37°C in the following medium: 4mM NADPH; 40mM sodium acetate buffer, pH 5.0; 4mM lead acetate and 5% sucrose.

QUALITATIVE AND QUANTITATIVE PHYTOCHEMICAL SCREENING AND IN VITRO ANTIOXIDANT EVALUATION OF PERGULARIA DAEMIA

2018 ◽  
Vol 24 (2) ◽  
Author(s):  
GITA MISHRA ◽  
HEMESHWER KUMAR CHANDRA ◽  
NISHA SAHU ◽  
SATENDRA KUMAR NIRALA ◽  
MONIKA BHADAURIA
Keyword(s):  
Reducing Sugars ◽  
Antioxidant Properties ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Qualitative And Quantitative ◽  
The Family ◽  
Ethanolic Extracts ◽  
Antioxidant Evaluation ◽  
In Vitro Antioxidant Activity

Pergularia daemia belongs to the family Asclepiadaceae, known to have anticancer, anti-inflammatory activity. Aim of the present study was to evaluate qualitative and quantitative phytochemical and antioxidant properties of ethanolic extracts of leaf, stem and root parts of P. daemia . Preliminary phytochemical analysis and in vitro antioxidant properties were evaluated by standard methods. The qualitative phytochemical analysis of P. daemia showed presence of flavonoids, tannins, alkaloid, phytosterol, carbohydrate, phenol, saponin, glycosides, terpenoids, steroids proteins and reducing sugars. Quantitative analysis showed polyphenol, flavonoid, flavonone, flavone and flavonol in P. daemia leaves, stem and root in considerable quantity. The in vitro antioxidant activity of P. daemia clearly demonstrated that leaf, stem and root parts have prominent antioxidant properties and was effective in scavenging free radicals.

Effects Attributed to Maleic Hydrazide when used for Chemical Sucker Control on Bright Tobacco

1980 ◽  
Vol 10 (2) ◽  
Author(s):  
Heinz Seltmann
Keyword(s):  
Statistical Difference ◽  
Reducing Sugars ◽  
Maleic Hydrazide ◽  
Water Soluble ◽  
Total Volatile ◽  
Total Alkaloids ◽  
Group I ◽  
Excellent Control ◽  
Per Se ◽  
Group Ii

AbstractThe effect of maleic hydrazide (MH) per se on bright tobacco was determined by comparing plants treated with MH to those without MH under conditions of good chemical sucker control. Sequential applications of each of five contact-type agents with MH one week later (Group I) were compared to dual applications of each of the same contact agents (Group II). In Group II suckers missed during applications were individually wetted to ensure excellent control. Sucker control was measured as 95 % for Group I and assumed to be 99 % for Group II. There were no agronomic differences between Groups I and II. In the visual warehouse appraisal, there was only a statistical difference for thin-bodied tobaccos between the two groups and a trend for slightly more heavy-bodied tobaccos in Group I. The chemical and physical analyses showed that filling value at 13 % moisture and equilibrium moisture content (EMC) measured at 60 % relative humidity were significantly lower in Group I than Group II. The result for EMC was questioned. Actual values for total alkaloids, total volatile bases minus nicotine, total ash, and alkalinity number of water-soluble ash were lower and reducing sugars were higher where MH was used. Except for EMC, the findings in this study reflected those established in studies where MH-treated and normally hand-suckered tobaccos were compared, but the differences here were generally not as great.

Effect of lead acetate on the nitric oxide system in rat tissue

2008 ◽  
Vol 21 (1) ◽  
pp. 175-178
Author(s):  
Isaac Trakhtenberg ◽  
Olena Apykhtina ◽  
Anatoly Kotsuruba ◽  
Julia Korkach ◽  
Irina Andrusishina
Keyword(s):  
Nitric Oxide ◽  
Lead Acetate ◽  
Oxide System ◽  
Rat Tissue ◽  
Nitric Oxide System

PHYSICOCHEMICAL AND MICROBIOLOGICAL EVALUATION OF HONEYS CONSUMED BY THE ELDERLY

2020 ◽  
Vol 5 ◽  
Author(s):  
Lusmarina Rodrigues Silva ◽  
Aline Marques Monte ◽  
Rafael Gomes Abreu Bacelar ◽  
Guilherme Antonio Silva Ribeiro ◽  
Aline Maria Dourado Rodrigues ◽  
...  
Keyword(s):  
Health Center ◽  
Reducing Sugars ◽  
The Elderly ◽  
Indicator Bacteria ◽  
Foreign Matter ◽  
Total Sugars ◽  
Significant Contamination ◽  
Almost All ◽  
Microbiological Analyses ◽  
Current Standards

Objective: to analyze physicochemical, microbiological and dirt parameters in marketed honeys, consumed by the elderly cared for at Integrated Health Center in Teresina, Piauí, Brazil. Method: the following analyses were performed: color, water activity, humidity, ash, pH, acidity, reducing sugars, total sugars, apparent sucrose and insoluble solids. Contamination indicator bacteria, mesophilic microorganisms, filamentous fungi and yeasts, as well as dirt and foreign matter, performed in the period from April to June 2016. Results: analyses of ash, pH, acidity and insoluble solids were outside current standards. Microbiological analyses did not present significant contamination. Also, analyses of dirt showed insect fragments, foreign matter in almost all the samples. Conclusion: parameters of ash, pH, acidity and insoluble solids, as well as dirt and foreign matter, indicated that the samples were not in accordance with current legislation. 

Sign in / Sign up

Export Citation Format

Share Document