The Interaction of Hydrogen Peroxide and Hypochlorous Acid in Acidic Solutions Containing Chloride Ion

1947 ◽  
Vol 69 (6) ◽  
pp. 1509-1514 ◽  
Author(s):  
Robert E. Connick
Keyword(s):  
Hydrogen Peroxide ◽  
Hypochlorous Acid ◽  
Chloride Ion ◽  
Acidic Solutions

Diagnostic Value of Myeloperoxidase Index in Bacterial Infections

2021 ◽  
Vol 27 (2) ◽  
pp. 164
Author(s):  
Mirna Rahmafindari ◽  
Paulus Budiono Notopuro ◽  
Betty Agustina Tambunan
Keyword(s):  
Hydrogen Peroxide ◽  
Predictive Value ◽  
Healthy Subjects ◽  
Bacterial Infections ◽  
Hypochlorous Acid ◽  
Chloride Ion ◽  
Diagnostic Value ◽  
Vital Role ◽  
Cross Sectional ◽  
Hematology Analyzer

Infectious diseases remain a serious problem in Indonesia. Myeloperoxidase (MPO) is a substance released by neutrophils, which activates the synthesis of hypochlorous acid (HOCL) from hydrogen peroxide (H2O2) and chloride ion (Cl-). Hypochlorous acid plays a vital role in the body's defense against infection. Myeloperoxidase Index (MPXI) is a parameter in the hematology analyzer Advia 2120i based on the principle of flow cytometry. This study aimed to determine the diagnostic value of MPXI in patients with bacterial infections. The study was a cross-sectional observational analysis. The samples consisted of a group of patients with bacterial infection and a group of healthy subjects. The specimens used in this study were whole blood + anticoagulant (EDTA) in a purple tube with a volume of 3 mL to determine the MPXI value in both groups using ADVIA 2120i hematology analyzer. The study subjects consisted of a group of patients with bacterial infections (69 patients) and a group of healthy subjects (33 people). Analysis of the MPXI ROC curve with a cut-off ≥ -5.8 and < -5.8, showed AUC of 0.323 (CI=95%, p=0.004), sensitivity of 34.8%, specificity of 39.4%, Positive Predictive Value (PPV) of 54.5%, and Negative Predictive Value (NPV) of 22.4%. Due to its low diagnostic value, the MPXI value was not recommended to be used as a diagnostic instrument for bacterial infections. Also, further research was highly needed.

Hyperoxia and self- or neutrophil-generated O2 metabolites inactivate xanthine oxidase

1988 ◽  
Vol 65 (5) ◽  
pp. 2349-2353 ◽  
Author(s):  
L. S. Terada ◽  
C. J. Beehler ◽  
A. Banerjee ◽  
J. M. Brown ◽  
M. A. Grosso ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Xanthine Oxidase ◽  
Superoxide Anion ◽  
Hypochlorous Acid ◽  
Control Mechanism ◽  
Xanthine Dehydrogenase ◽  
Biological Processes ◽  
Lung Endothelial Cells ◽  
Cellular Control

Xanthine oxidase (XO) and xanthine dehydrogenase (XD) activities decreased in lungs isolated from rats and cultured lung endothelial cells that had been exposed to hyperoxia. Purified XO activity also decreased after addition of a variety of chemically generated O2 metabolite species (superoxide anion, hydrogen peroxide, hydroxyl radical, or hypochlorous acid), hypoxanthine, or stimulated neutrophils in vitro. XO inactivation by chemically, self-, or neutrophil-generated O2 metabolites was decreased by simultaneous addition of various O2 metabolite scavengers but not their inactive analogues. Since XO appears to contribute to a variety of biological processes and diseases, hyperoxia- or O2 metabolite-mediated decreases in XO activity may be an important cellular control mechanism.

scholarly journals Myeloperoxidase Modulates Hydrogen Peroxide Mediated Cellular Damage in Murine Macrophages

Antioxidants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1255
Author(s):  
Chaorui Guo ◽  
Inga Sileikaite ◽  
Michael J. Davies ◽  
Clare L. Hawkins
Keyword(s):  
Hydrogen Peroxide ◽  
Hypochlorous Acid ◽  
Cell Function ◽  
Inflammatory Diseases ◽  
Innate Immune ◽  
Cellular Damage ◽  
Enzymatic System ◽  
Therapeutic Approaches ◽  
Macrophage Cell

Myeloperoxidase (MPO) is involved in the development of many chronic inflammatory diseases, in addition to its key role in innate immune defenses. This is attributed to the excessive production of hypochlorous acid (HOCl) by MPO at inflammatory sites, which causes tissue damage. This has sparked wide interest in the development of therapeutic approaches to prevent HOCl-induced cellular damage including supplementation with thiocyanate (SCN−) as an alternative substrate for MPO. In this study, we used an enzymatic system composed of glucose oxidase (GO), glucose, and MPO in the absence and presence of SCN−, to investigate the effects of generating a continuous flux of oxidants on macrophage cell function. Our studies show the generation of hydrogen peroxide (H2O2) by glucose and GO results in a dose- and time-dependent decrease in metabolic activity and cell viability, and the activation of stress-related signaling pathways. Interestingly, these damaging effects were attenuated by the addition of MPO to form HOCl. Supplementation with SCN−, which favors the formation of hypothiocyanous acid, could reverse this effect. Addition of MPO also resulted in upregulation of the antioxidant gene, NAD(P)H:quinone acceptor oxidoreductase 1. This study provides new insights into the role of MPO in the modulation of macrophage function, which may be relevant to inflammatory pathologies.

A kinetic study of the oxidation of N(III) by ClO2− in aqueous acidic media

1980 ◽  
Vol 58 (2) ◽  
pp. 159-163 ◽  
Author(s):  
Samir S. Emeish ◽  
Keith E. Howlett
Keyword(s):  
Kinetic Study ◽  
Rate Equation ◽  
Nitrous Acid ◽  
Hypochlorous Acid ◽  
Chloride Ion ◽  
Acidic Media ◽  
Kinetics Of

Kinetics of the reaction between nitrous acid and chlorite have been studied spectrophotometrically. The rate equation involves terms [HNO2]1.5 and [ClO2−]0.5, and both an uncatalysed and a chloride ion-catalysed path have been identified. A mechanism involving peroxonitrite and hypochlorous acid as intermediates is shown to be consistent with the observations.

Contrasting Effects of Hypochlorous Acid and Hydrogen Peroxide on Endothelial Permeability

1997 ◽  
Vol 156 (4) ◽  
pp. 1247-1255 ◽  
Author(s):  
LUIS OCHOA ◽  
GREGORY WAYPA ◽  
JOHN R. MAHONEY ◽  
LUIS RODRIGUEZ ◽  
FRED L. MINNEAR
Keyword(s):  
Hydrogen Peroxide ◽  
Hypochlorous Acid ◽  
Endothelial Permeability
Sign in / Sign up

Export Citation Format

Share Document