Effects of the reactive oxygen species hypochlorous acid and hydrogen peroxide on force production and calcium sensitivity of rat cardiac myofilaments

1994 ◽  
Vol 428 (5-6) ◽  
pp. 561-568 ◽  
Author(s):  
Niall G. MacFarlane ◽  
David J. Miller
Keyword(s):  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Hypochlorous Acid ◽  
Force Production ◽  
Reactive Oxygen ◽  
Calcium Sensitivity ◽  
Oxygen Species

Tryptophan Metabolites as Scavengers of Reactive Oxygen and Chlorine Species

Pteridines ◽  
2002 ◽  
Vol 13 (4) ◽  
pp. 140-143 ◽  
Author(s):  
Günter Weiss ◽  
Antonio Diez-Ruiz ◽  
Christian Murr ◽  
Igor Theur ◽  
Dietmar Fuchs
Keyword(s):  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Hypochlorous Acid ◽  
Reactive Oxygen ◽  
Human Monocyte ◽  
Interferon Γ ◽  
Oxygen Species ◽  
Tryptophan Degradation ◽  
Tryptophan Metabolites

Abstract Upon stimulation with interferon-γ, a typical Thl cell-derived cytokine, human monocyte-dertved macrophages produce neopterin derivatives and in parallel degrade the essential amino acid L-tryptophan to L-kynurenine and subsequently to 3-hydroxyanthramlic acid and anthramlic acid. In parallel, stimulated macrophages produce reactive oxygen species such as hydrogen peroxide and hypochlorous acid. Earlier, neopterin and 7.8-dihydroneoptenn were found to enhance or decrease effects of reactive oxygen species in vitro, depending on concentration and on environmental condition. In this study, we investigated the ability of tryptophan and its metabolites to interfere with radicals in vitro by means of a chemiluminiseence-based assay system. When using hydrogen peroxide or chloramine Τ as source for radical formation. L-tryptophan and its catabolites reduced chennluminescence according to a dose-response relationship, 3-hydroxvanthranilic acid being the most efficient compound. Apart from L-kynurenme the scavenging effects of tryptophan and its metabolites were not affected by changes m pH from 5.5 to 7.5. Our data indicate that tryptophan degradation produces metabolites with a high scavenging ability for reactive oxygen and chlorine species, thereby establishing a self-regulatory mechanism to limit the tissue damage by reactive radicals produced by macrophages.

scholarly journals Reactive oxygen species and oocyte aging: Role of superoxide, hydrogen peroxide, and hypochlorous acid

2008 ◽  
Vol 44 (7) ◽  
pp. 1295-1304 ◽  
Author(s):  
Anuradha P. Goud ◽  
Pravin T. Goud ◽  
Michael P. Diamond ◽  
Bernard Gonik ◽  
Husam M. Abu-Soud
Keyword(s):  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Hypochlorous Acid ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Oocyte Aging

Superoxide, hydroxyl radical, and hydrogen peroxide effects on single-diaphragm fiber contractile apparatus

2001 ◽  
Vol 90 (1) ◽  
pp. 45-54 ◽  
Author(s):  
L. A. Callahan ◽  
Z. W. She ◽  
T. M. Nosek
Keyword(s):  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Hydroxyl Radical ◽  
Reactive Oxygen ◽  
Calcium Sensitivity ◽  
Oxygen Species ◽  
Contractile Apparatus ◽  
Skeletal Muscle Function ◽  
Bathing Medium ◽  
Specific Species

Reactive oxygen species contribute to diaphragm dysfunction in certain pathophysiological conditions (i.e., sepsis and fatigue). However, the precise alterations induced by reactive oxygen species or the specific species that are responsible for the derangements in skeletal muscle function are incompletely understood. In this study, we evaluated the effect of the superoxide anion radical (O2 −·), hydroxyl radical (·OH), and hydrogen peroxide (H2O2) on maximum calcium-activated force (Fmax) and calcium sensitivity of the contractile apparatus in chemically skinned (Triton X-100) single rat diaphragm fibers. O2 −· was generated using the xanthine/xanthine oxidase system; ·OH was generated using 1 mM FeCl2, 1 mM ascorbate, and 1 mM H2O2; and H2O2 was added directly to the bathing medium. Exposure to O2 −· or ·OH significantly decreased Fmax by 14.5% ( P < 0.05) and 43.9% ( P < 0.005), respectively. ·OH had no effect on Ca2+ sensitivity. Neither 10 nor 1,000 μM H2O2 significantly altered Fmax or Ca2+ sensitivity. We conclude that the diaphragm is susceptible to alterations induced by a direct effect of ·OH and O2 −·, but not H2O2, on the contractile proteins, which could, in part, be responsible for prolonged depression in contractility associated with respiratory muscle dysfunction in certain pathophysiological conditions.

Hydrogen peroxide-responsive AIE probe for imaging-guided organelle targeting and photodynamic cancer cell ablation

2021 ◽  
Author(s):  
Qian Wu ◽  
Youmei Li ◽  
Ying Li ◽  
Dong Wang ◽  
Ben Zhong Tang
Keyword(s):  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Cancer Cell ◽  
Reactive Oxygen ◽  
Living Cells ◽  
Oxygen Species ◽  
Cell Ablation ◽  
Selective Monitoring ◽  
Organelle Targeting

Hydrogen peroxide (H2O2), as one kind of key reactive oxygen species (ROS), is mainly produced endogenously primarily in the mitochondria. The selective monitoring of H2O2 in living cells is of...

scholarly journals Upconversion-based nanosystems for fluorescence sensing of pH and H2O2

2021 ◽  
Author(s):  
Chunning Sun ◽  
Michael Gradzielski
Keyword(s):  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Excitation Energy ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Fluorescence Sensing ◽  
Living Organisms

Hydrogen peroxide (H2O2), a key reactive oxygen species, plays an important role in living organisms, industrial and environmental fields. Here, a non-contact upconversion nanosystem based on the excitation energy attenuation...

scholarly journals Novel Antioxidant Properties of Doxycycline

2018 ◽  
Vol 19 (12) ◽  
pp. 4078 ◽  
Author(s):  
Dahn Clemens ◽  
Michael Duryee ◽  
Cleofes Sarmiento ◽  
Andrew Chiou ◽  
Jacob McGowan ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Chronic Inflammation ◽  
Antioxidant Properties ◽  
Antibacterial Properties ◽  
Reactive Oxygen ◽  
Protein Adducts ◽  
Oxygen Species ◽  
Free System

Doxycycline (DOX), a derivative of tetracycline, is a broad-spectrum antibiotic that exhibits a number of therapeutic activities in addition to its antibacterial properties. For example, DOX has been used in the management of a number of diseases characterized by chronic inflammation. One potential mechanism by which DOX inhibits the progression of these diseases is by reducing oxidative stress, thereby inhibiting subsequent lipid peroxidation and inflammatory responses. Herein, we tested the hypothesis that DOX directly scavenges reactive oxygen species (ROS) and inhibits the formation of redox-mediated malondialdehyde-acetaldehyde (MAA) protein adducts. Using a cell-free system, we demonstrated that DOX scavenged reactive oxygen species (ROS) produced during the formation of MAA-adducts and inhibits the formation of MAA-protein adducts. To determine whether DOX scavenges specific ROS, we examined the ability of DOX to directly scavenge superoxide and hydrogen peroxide. Using electron paramagnetic resonance (EPR) spectroscopy, we found that DOX directly scavenged superoxide, but not hydrogen peroxide. Additionally, we found that DOX inhibits MAA-induced activation of Nrf2, a redox-sensitive transcription factor. Together, these findings demonstrate the under-recognized direct antioxidant property of DOX that may help to explain its therapeutic potential in the treatment of conditions characterized by chronic inflammation and increased oxidative stress.

A Vinyl-Boronate Ester-Based Persulfide Donor Controllable by Hydrogen Peroxide, a Reactive Oxygen Species (ROS)

2018 ◽  
Vol 20 (24) ◽  
pp. 7916-7920 ◽  
Author(s):  
Prerona Bora ◽  
Preeti Chauhan ◽  
Suman Manna ◽  
Harinath Chakrapani
Keyword(s):  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Boronate Ester

A charge transfer assisted fluorescent probe for selective detection of hydrogen peroxide among different reactive oxygen species

2012 ◽  
Vol 48 (39) ◽  
pp. 4719 ◽  
Author(s):  
Manoj Kumar ◽  
Naresh Kumar ◽  
Vandana Bhalla ◽  
Parduman Raj Sharma ◽  
Yasrib Qurishi
Keyword(s):  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Charge Transfer ◽  
Fluorescent Probe ◽  
Reactive Oxygen ◽  
Selective Detection ◽  
Oxygen Species ◽  
A Charge

scholarly journals Caffeic Acid - Potential Antioxidant in Cultured Human Retinal Pigment Epithelial Cells

1970 ◽  
Vol 66 (2) ◽  
Author(s):  
Dumitriţa RUGINǍ ◽  
Adela PINTEA ◽  
Raluca PÂRLOG ◽  
Andreea VARGA
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Protective Effect ◽  
Caffeic Acid ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Antioxidant Defence ◽  
Rpe Cells ◽  
In Cells

Oxidative stress causes biological changes responsible for carcinogenesis and aging in human cells. The retinal pigmented epithelium is continuously exposed to oxidative stress. Therefore reactive oxygen species (ROS) and products of lipid peroxidation accumulate in RPE. Neutralization of ROS occurs in retina by the action of antioxidant defence systems. In the present study, the protective effect of caffeic acid (3,4-dihydroxy cinnamic acid), a dietary phenolic compound, has been examined in normal and in oxidative stress conditions (500 µM peroxide oxygen) in cultures human epithelial pigment retinal cells (Nowak, M. et al.). The cell viability, the antioxidant enzymes activity (CAT, GPx, SOD) and the level of intracellular reactive oxygen species (ROS) were determined. Exposure to l00 µM caffeic acid for 24 h induced cellular changes indicating the protective effect of caffeic acid in RPE cells. Caffeic acid did not show any cytotoxic effect at concentrations lower than 200 μM in culture medium. Treatment of RPE cells with caffeic acid causes an increase of catalase, glutathione peroxidase and superoxide dismutase activity, especially in cells treated with hydrogen peroxide. Caffeic acid causes a decrease of ROS level in cells treated with hydrogen peroxide. This study proved that caffeic acid or food that contain high levels of this phenolic acid may have beneficial effects in prevention of retinal diseases associated with oxidative stress by improving antioxidant defence systems.

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