Kinetic Evidence on the Mechanism of the Acid Denaturation of Horse CO Hemoglobin1

1951 ◽  
Vol 73 (12) ◽  
pp. 5568-5572 ◽  
Author(s):  
Ethel M. Zaiser ◽  
Jacinto Steinhardt
Keyword(s):  
Acid Denaturation

The Acid denaturation of the proteins of the groundnut (arachis hypogaea)

2007 ◽  
Vol 8 (9) ◽  
pp. 598-605 ◽  
Author(s):  
W. E. F. Naismith ◽  
Kirsteen O. Kelly
Keyword(s):  
Arachis Hypogaea ◽  
Acid Denaturation

Reversibility of acid denaturation of recombinant interferon-?

Biopolymers ◽  
1990 ◽  
Vol 29 (6-7) ◽  
pp. 1065-1068 ◽  
Author(s):  
Tsutomu Arakawa ◽  
Yeuh-Rong Hsu ◽  
Michael A. Narachi ◽  
Charles Herrera ◽  
Michael F. Rohde ◽  
...  
Keyword(s):  
Recombinant Interferon ◽  
Acid Denaturation

Schweres Wasser (D2O) als Protektivum proteinhaltiger Arzneimittel. Beispiel: humane Cholinesterase/Protein Drugs Stabilized by Heavy Water (D2O). For Example: Human Cholinesterase

1991 ◽  
Vol 46 (9-10) ◽  
pp. 789-793 ◽  
Author(s):  
Ch. Heyde ◽  
M. Wenzel
Keyword(s):  
Protective Effect ◽  
Heavy Water ◽  
Protein Drugs ◽  
Acid Denaturation ◽  
Individual Effects ◽  
The Individual ◽  
Human Cholinesterase

Abstract Looking for a possible protective effect of heavy water on proteins, cholinesterase and lac­tate dehydrogenase in combination with D2O and further protective substances were exposed to a temperature of 60 °C (for 10 min) and about 45 °C (for several days). In combination with glycerine there resulted an additive protective effect; with NaCl and/or albumin being added the individual effects raised to a higher level. In the cold D2O protects cholinesterase only against acid denaturation, but in combination with warmth also against basic denaturation.

Significance of the initial fast reaction in the acid denaturation of ferrihemoglobins

Biochemistry ◽  
1969 ◽  
Vol 8 (12) ◽  
pp. 5075-5081 ◽  
Author(s):  
John W. Allis ◽  
Jacinto Steinhardt
Keyword(s):  
Fast Reaction ◽  
Acid Denaturation

THE ACID DENATURATION OF FERRIHEMOGLOBIN1

1957 ◽  
Vol 79 (14) ◽  
pp. 3931-3932 ◽  
Author(s):  
Charles Tanford
Keyword(s):  
Acid Denaturation

The Stabilization of Horse Ferrihemoglobin to Acid Denaturation by Combination with Ligands*

Biochemistry ◽  
1963 ◽  
Vol 2 (2) ◽  
pp. 256-266 ◽  
Author(s):  
J. Steinhardt ◽  
R. Ona-Pascual ◽  
S. Beychok ◽  
Chien Ho
Keyword(s):  
Acid Denaturation

Methyl green-pyronin stain distinguishes proliferating from differentiated nonproliferating cell nuclei after acid denaturation of DNA.

1986 ◽  
Vol 34 (5) ◽  
pp. 683-687 ◽  
Author(s):  
S Iseki ◽  
T Mori
Keyword(s):  
Basal Layer ◽  
Methyl Green ◽  
Esophageal Mucosa ◽  
Cell Renewal ◽  
Cell Nuclei ◽  
Proliferating Cells ◽  
Selective Staining ◽  
Acid Denaturation ◽  
Dna And Rna ◽  
Granular Layers

We applied methyl green-pyronin (MG-P) stain, which is usually used for the selective staining of DNA and RNA, to frozen sections of rat jejunal and esophageal mucosa, following digestion with RNase and treatment with various concentrations of HCl. The pyroninophilia of the nuclei increased with increasing strength of the acid, but the susceptibility of the nuclei to acid differed among cell populations. In the jejunal epithelium, at an appropriate acid strength the nuclei in the crypts of Lieberkuhn were less acid-sensitive and remained blue-green, whereas those in the villi were more pyroninophilic and stained lavender. Under the same conditions, the nuclei in the basal layer of the esophageal epithelium were blue-green and those in the spinous and granular layers were increasingly lavender. These results suggest that in cell-renewal systems the differentiated, nonproliferating cells are more sensitive to acid denaturation of DNA than the undifferentiated, actively proliferating cells. MG-P stain, which is able to distinguish double-stranded from single-stranded DNA, may be used as a tool to stain proliferating and nonproliferating cell nuclei differentially in tissue sections.

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