Biochemical Studies on Vitamin A. IX. Biopotency of Neovitamin A in the Rat

1951 ◽  
Vol 73 (3) ◽  
pp. 1252-1254 ◽  
Author(s):  
Philip L. Harris ◽  
Stanley R. Ames ◽  
John H. Brinkman
1955 ◽  
Vol 77 (15) ◽  
pp. 4136-4138 ◽  
Author(s):  
Stanley R. Ames ◽  
William J. Swanson ◽  
Philip L. Harris

1952 ◽  
Vol 29 (4) ◽  
pp. 151-153 ◽  
Author(s):  
Stanley R. Ames ◽  
Marion I. Ludwig ◽  
William J. Swanson ◽  
Philip L. Harris

1955 ◽  
Vol 77 (15) ◽  
pp. 4134-4136 ◽  
Author(s):  
Stanley R. Ames ◽  
William J. Swanson ◽  
Philip L. Harris

Author(s):  
Odell T. Minick ◽  
Hidejiro Yokoo ◽  
Fawzia Batti

Vacuolated cells in the liver of young rats were studied by light and electron microscopy following the administration of vitamin A (200 units per gram of body weight). Their characteristics were compared with similar cells found in untreated animals.In rats given vitamin A, cells with vacuolated cytoplasm were a prominent feature. These cells were found mostly in a perisinusoidal location, although some appeared to be in between liver cells (Fig. 1). Electron microscopy confirmed their location in Disse's space adjacent to the sinusoid and in recesses between liver cells. Some appeared to be bordering the lumen of the sinusoid, but careful observation usually revealed a tenuous endothelial process separating the vacuolated cell from the vascular space. In appropriate sections, fenestrations in the thin endothelial processes were noted (Fig. 2, arrow).


Author(s):  
M. C. Buhrer ◽  
R. A. Mathews

Ruthenium red has been used as a stain to demonstrate a variety of extracellular materials, especially acid mucopolysaccharides. It also reacts with certain intracellular and extracellular lipids. Since biochemical studies in our laboratory demonstrated the presence of a variety of monosaccharides in human hair ruthenium red staining procedures were adopted in order to evaluate the presence and morphological location of acid oligosaccharides in the keratinized aspect of hair.


Author(s):  
Robert R. Cardell

Hypophysectomy of the rat renders this animal deficient in the hormones of the anterior pituitary gland, thus causing many primary and secondary hormonal effects on basic liver functions. Biochemical studies of these alterations in the rat liver cell are quite extensive; however, relatively few morphological observations on such cells have been recorded. Because the available biochemical information was derived mostly from disrupted and fractionated liver cells, it seemed desirable to examine the problem with the techniques of electron microscopy in order to see what changes are apparent in the intact liver cell after hypophysectomy. Accordingly, liver cells from rats which had been hypophysectomized 5-120 days before sacrifice were studied. Sham-operated rats served as controls and both hypophysectomized and control rats were fasted 15 hours before sacrifice.


Author(s):  
J.C.S. Kim ◽  
M.G. Jourden ◽  
E.S. Carlisle

Chronic exposure to nitrogen dioxide in rodents has shown that injury reaches a maximum after 24 hours, and a reparative adaptive phase follows (1). Damage occurring in the terminal bronchioles and proximal portions of the alveolar ducts in rats has been extensively studied by both light and electron microscopy (1).The present study was undertaken to compare the response of lung tissue to intermittent exposure to 10 ppm of nitrogen dioxide gas for 4 hours per week, while the hamsters were on a vitamin A deficient diet. Ultrastructural observations made from lung tissues obtained from non-gas exposed, hypovitaminosis A animals and gas exposed animals fed a regular commercially prepared diet have been compared to elucidate the specific effect of vitamin A on nitrogen dioxide gas exposure. The interaction occurring between vitamin A and nitrogen dioxide gas has not previously been investigated.


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