Proteins as random coils. III. Optical rotatory dispersion in 6M guanidine hydrochloride

1967 ◽  
Vol 89 (19) ◽  
pp. 5023-5029 ◽  
Author(s):  
Charles. Tanford ◽  
Kazuo. Kawahara ◽  
Savo. Lapanje ◽  
Thomas M. Hooker ◽  
Mario H. Zarlengo ◽  
...  
Keyword(s):  
Guanidine Hydrochloride ◽  
Optical Rotatory Dispersion ◽  
Rotatory Dispersion ◽  
Optical Rotatory ◽  
Random Coils

scholarly journals Sedimentation Equilibrium Studies on Protein From Kookaburra Beak

1976 ◽  
Vol 29 (6) ◽  
pp. 481
Author(s):  
EF Woods
Keyword(s):  
Molecular Weight ◽  
Optical System ◽  
Optical Rotatory Dispersion ◽  
Rotatory Dispersion ◽  
Sedimentation Equilibrium ◽  
Optical Rotatory ◽  
Equilibrium Studies ◽  
Aqueous Buffer ◽  
Random Coils ◽  
Equilibrium Sedimentation

Fractionated samples of the soluble S-carboxymethyl proteins from kookaburra beak (Frenkel and Gillespie 1976) were examined by equilibrium sedimentation. The molecular weight was found to be 11 300 when the photoelectric scanning absorption optical system was employed and 13 700 when Rayleigh interference optics were used. Possible explanations for this difference are considered and it is concluded that it must arise from heterogeneity of the protein. Optical rotatory dispersion measurements indicate that the proteins probably exist as random coils in dilute aqueous buffer.

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