Orbital mixing rule

1976 ◽  
Vol 98 (14) ◽  
pp. 4054-4061 ◽  
Author(s):  
Satoshi Inagaki ◽  
Hiroshi Fujimoto ◽  
Kenichi Fukui
Keyword(s):  
Mixing Rule ◽  
Orbital Mixing

Orbital Mixing Rule

1997 ◽  
pp. 309-316
Author(s):  
SATOSHI INAGAKI ◽  
HIROSHI FUJIMOTO ◽  
KENICHI FUKUI
Keyword(s):  
Mixing Rule ◽  
Orbital Mixing

ChemInform Abstract: ORBITAL MIXING RULE

1976 ◽  
Vol 7 (39) ◽  
pp. no-no
Author(s):  
SATOSHI INAGAKI ◽  
HIROSHI FUJIMOTO ◽  
KENICHI FUKUI
Keyword(s):  
Mixing Rule ◽  
Orbital Mixing

scholarly journals An improved vdW-type component mixing-rule applied in CO 2 -crude system

2016 ◽  
Vol 43 (3) ◽  
pp. 484-489 ◽  
Author(s):  
Siyu YANG ◽  
Liming LIAN ◽  
Shi LI ◽  
Yongzhi YANG ◽  
Xinglong CHEN
Keyword(s):  
Mixing Rule

scholarly journals Microplate Orbital Mixing Improves High-Throughput Cell-Based Reporter Assay Readouts

2006 ◽  
Vol 12 (1) ◽  
pp. 140-144 ◽  
Author(s):  
Michael K. Hancock ◽  
Myleen N. Medina ◽  
Brendan M. Smith ◽  
Anthony P. Orth
Keyword(s):  
High Throughput ◽  
Dynamic Range ◽  
Cell Lysis ◽  
Single Step ◽  
Luciferase Assay ◽  
Luciferase Reporter ◽  
Activity Measurements ◽  
Simple Detection ◽  
Reporter Assays ◽  
Orbital Mixing

Reporter assays are commonly used for high-throughput cell-based screening of compounds, cDNAs, and siRNAs due to robust signal, ease of miniaturization, and simple detection and analysis. Among the most widely used reporter genes is the bioluminescent enzyme luciferase, which, when exposed to its substrate luciferin upon cell lysis, yields linear signal over a dynamic range of several orders of magnitude. Commercially available luciferase assay formulations have been developed permitting homogeneous, single-step cell lysis and reporter activity measurements. Assay conditions employed with these formulations are typically designed to minimize well-to-well luminescence variability due to variability in dispensing, evaporation, and incomplete sample mixing. The authors demonstrate that incorporating a microplate orbital mixing step into 96- and 384-well microplate cell-based luciferase reporter assays can greatly improve reporter readouts. They have found that orbital mixing using commercially available mixers facilitates maximal luciferase signal generation from high cell density–containing samples while minimizing variability due to partial cell lysis, thereby improving assay precision. The authors fully expect that widespread availability of mixers with sufficiently small orbits and higher speed settings will permit gains in signal and precision in the 1536-well format as well.

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