Thermolysis of bis[2-[(trimethylsilyl)oxy]propyl]furoxan (TOP-furoxan). The first practical method for intermolecular cycloaddition of an in situ generated nitrile oxide with 1,2-di- and trisubstituted olefins

1985 ◽  
Vol 107 (21) ◽  
pp. 6023-6028 ◽  
Author(s):  
Dennis P. Curran ◽  
Christopher J. Fenk
Keyword(s):  
Practical Method ◽  
Nitrile Oxide ◽  
Trisubstituted Olefins

Hypervalent-Iodine Mediated One-Pot Synthesis of Isoxazolines and Isoxazoles bearing Difluoromethyl Phosphonate Moiety

2021 ◽  
Author(s):  
Romana Pajkert ◽  
Henryk Koroniak ◽  
Pawel Kafarski ◽  
Gerd Volker Roeschenthaler
Keyword(s):  
Nitrile Oxide ◽  
Dipolar Cycloaddition ◽  
Hypervalent Iodine ◽  
One Pot ◽  
One Pot Synthesis

A one-pot, regioselective 1,3-dipolar cycloaddition of in situ generated (diethoxyphosphoryl)difluoromethyl nitrile oxide toward selected alkenes and alkynes is reported. This protocol enables facile access to 3,5-disubstituted isoxazolines and isoxazoles bearing...

Nitrile Oxide Cycloaddition Chemistry Using Benzotriazole as a Steric Auxiliary

2005 ◽  
Vol 58 (12) ◽  
pp. 877 ◽  
Author(s):  
G. Paul Savage ◽  
Gregory T. Wernert
Keyword(s):  
Nitrile Oxide

The relatively hindered 2,2-bis(benzotriazol-1-yl)acetonitrile oxide was prepared in situ from the precursor hydroximinoyl chloride, and was allowed to react with dipolarophiles to give rise to several isoxazoles. The benzotriazole substituents acted as steric auxiliaries to prevent unwanted dimerization of the nitrile oxide to the furoxan by-product.

In situ-forming click-crosslinked gelatin based hydrogels for 3D culture of thymic epithelial cells

2016 ◽  
Vol 4 (7) ◽  
pp. 1123-1131 ◽  
Author(s):  
Vinh X. Truong ◽  
Michael L. Hun ◽  
Fanyi Li ◽  
Ann P. Chidgey ◽  
John S. Forsythe
Keyword(s):  
Epithelial Cells ◽  
Click Reaction ◽  
3D Culture ◽  
Nitrile Oxide ◽  
Thymic Epithelial Cells ◽  
In Situ Forming ◽  
Culture Environment

In situ-forming gelatin based hydrogels, which are crosslinked using an efficient nitrile oxide-norbornene click reaction, provide a suitable 3D culture environment for thymic epithelial cells.

scholarly journals Nanopore-mediated protein delivery enabling three-color single-molecule tracking in living cells

2021 ◽  
Vol 118 (5) ◽  
pp. e2012229118
Author(s):  
Zhongwen Chen ◽  
Yuhong Cao ◽  
Chun-Wei Lin ◽  
Steven Alvarez ◽  
Dongmyung Oh ◽  
...  
Keyword(s):  
Single Molecule ◽  
Protein Delivery ◽  
Chemical Properties ◽  
Practical Method ◽  
Living Cells ◽  
Egfr Signaling ◽  
Single Molecule Tracking ◽  
Ras Activation ◽  
And Chemical Properties

Multicolor single-molecule tracking (SMT) provides a powerful tool to mechanistically probe molecular interactions in living cells. However, because of the limitations in the optical and chemical properties of currently available fluorophores and the multiprotein labeling strategies, intracellular multicolor SMT remains challenging for general research studies. Here, we introduce a practical method employing a nanopore-electroporation (NanoEP) technique to deliver multiple organic dye-labeled proteins into living cells for imaging. It can be easily expanded to three channels in commercial microscopes or be combined with other in situ labeling methods. Utilizing NanoEP, we demonstrate three-color SMT for both cytosolic and membrane proteins. Specifically, we simultaneously monitored single-molecule events downstream of EGFR signaling pathways in living cells. The results provide detailed resolution of the spatial localization and dynamics of Grb2 and SOS recruitment to activated EGFR along with the resultant Ras activation.

scholarly journals Practical Method to Screen Contaminated Holograms of Flocs Using Light Intensity

2021 ◽  
Vol 8 ◽  
Author(s):  
Sun Min Choi ◽  
Jun Young Seo ◽  
Guan-hong Lee ◽  
Xiaoteng Shen ◽  
Ho Kyung Ha
Keyword(s):  
Light Intensity ◽  
Practical Method ◽  
Accurate Estimation ◽  
Cohesive Sediments ◽  
Degree Of Contamination ◽  
Particle Properties ◽  
And Behavior ◽  
Biological Organisms ◽  
Number Of Particles

Submergible digital holographic camera can measure the in situ size and shape of suspended particles, such as complex flocs and biological organisms, without disturbance. As the number of particles in the water column increases, overlapping concentric rings (interference patterns) can contaminate the holographic images. Using light intensity (LI), this study proposes a practical method to assess the degree of contamination and screen out contaminated images. The outcomes from image processing support that LI normalized on a gray scale of 0 (black) to 255 (white) can be a reliable criterion for defining the contamination boundary. Results found that as LI increased, the shape of the particle size distribution shifted from a positively skewed to a normal distribution. When LI was lower than approximately 80, owing to the distortion of particle properties, the settling velocities derived from the contaminated holograms with mosaic patterns were underestimated compared to those from the uncontaminated holograms. The proposed method can contribute to a more accurate estimation of the transport and behavior of cohesive sediments in shallow estuarine environments.

Catalyst-free formation of 1,4-diketones by addition of silyl enolates to oxyallyl zwitterions in situ generated from α-haloketones

RSC Advances ◽  
2015 ◽  
Vol 5 (83) ◽  
pp. 67901-67908 ◽  
Author(s):  
Juan Luo ◽  
Qihua Jiang ◽  
Hao Chen ◽  
Qiang Tang
Keyword(s):  
Room Temperature ◽  
Practical Method ◽  
Direct Coupling ◽  
Catalyst Free

Here we report an efficient and practical method for the preparation of 1,4-diketones by direct coupling of α-haloketones with silyl enolates at room temperature. No catalysts were required in our protocol.

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