In situ-forming click-crosslinked gelatin based hydrogels for 3D culture of thymic epithelial cells

2016 ◽  
Vol 4 (7) ◽  
pp. 1123-1131 ◽  
Author(s):  
Vinh X. Truong ◽  
Michael L. Hun ◽  
Fanyi Li ◽  
Ann P. Chidgey ◽  
John S. Forsythe
Keyword(s):  
Epithelial Cells ◽  
Click Reaction ◽  
3D Culture ◽  
Nitrile Oxide ◽  
Thymic Epithelial Cells ◽  
In Situ Forming ◽  
Culture Environment

In situ-forming gelatin based hydrogels, which are crosslinked using an efficient nitrile oxide-norbornene click reaction, provide a suitable 3D culture environment for thymic epithelial cells.

scholarly journals Human thymic epithelial cells produce interleukin-3

Blood ◽  
1991 ◽  
Vol 77 (1) ◽  
pp. 69-74 ◽  
Author(s):  
AH Dalloul ◽  
M Arock ◽  
C Fourcade ◽  
A Hatzfeld ◽  
JM Bertho ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Epithelial Cells ◽  
Culture Conditions ◽  
Thymic Epithelial Cells ◽  
Interleukin 3 ◽  
Activated T Lymphocytes ◽  
Exclusive Property ◽  
Thymic Stroma ◽  
Culture Supernatants

Abstract Interleukin-3 (IL-3) is a hematopoietic growth factor suggested to be produced by activated T lymphocytes. Meanwhile, supernatants from human thymic stroma could promote the proliferation of myeloid stem cells. Thus, we investigated whether IL-3 accounts for this activity. Therefore, human thymic epithelial cells (TEC), fibroblasts, and adherent cells were isolated, and their culture supernatants assayed for myeloid colony promotion. Only supernatants from thymic epithelial cells supported colony-forming unit growth in semisolid media. This effect decreased following anti-IL-3 monoclonal antibody addition to these cultures. Furthermore, in situ hybridization showed the presence of IL-3 mRNA in epithelial cells. Effect of TEC culture conditions on IL-3 production by these cells was also studied. Together, these data show that IL-3 production is not the exclusive property of human activated T lymphocytes.

scholarly journals In situ-forming robust chitosan-poly(ethylene glycol) hydrogels prepared by copper-free azide–alkyne click reaction for tissue engineering

2014 ◽  
Vol 2 (2) ◽  
pp. 167-175 ◽  
Author(s):  
Vinh X. Truong ◽  
Matthew P. Ablett ◽  
Hamish T. J. Gilbert ◽  
James Bowen ◽  
Stephen M. Richardson ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Ethylene Glycol ◽  
Click Reaction ◽  
Poly Ethylene Glycol ◽  
In Situ Forming ◽  
Poly Ethylene

In situ evidence of cellular senescence in Thymic Epithelial Cells (TECs) during human thymic involution

2019 ◽  
Vol 177 ◽  
pp. 88-90 ◽  
Author(s):  
Alexandra Barbouti ◽  
Konstantinos Evangelou ◽  
Ioannis S. Pateras ◽  
Alexandra Papoudou-Bai ◽  
Amalia Patereli ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Cellular Senescence ◽  
Thymic Epithelial Cells ◽  
Thymic Involution

Rat thymic epithelial cells in vitro and in situ: characterization by immunocytochemistry and morphology

1996 ◽  
Vol 283 (2) ◽  
pp. 221-229 ◽  
Author(s):  
Bodo Kurz ◽  
Brita von Gaudecker ◽  
Brigitte Krisch ◽  
Rolf Mentlein
Keyword(s):  
Epithelial Cells ◽  
Thymic Epithelial Cells ◽  
In Situ Characterization

scholarly journals Human thymic epithelial cells produce interleukin-3

Blood ◽  
1991 ◽  
Vol 77 (1) ◽  
pp. 69-74
Author(s):  
AH Dalloul ◽  
M Arock ◽  
C Fourcade ◽  
A Hatzfeld ◽  
JM Bertho ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Epithelial Cells ◽  
Culture Conditions ◽  
Thymic Epithelial Cells ◽  
Interleukin 3 ◽  
Activated T Lymphocytes ◽  
Exclusive Property ◽  
Thymic Stroma ◽  
Culture Supernatants

Interleukin-3 (IL-3) is a hematopoietic growth factor suggested to be produced by activated T lymphocytes. Meanwhile, supernatants from human thymic stroma could promote the proliferation of myeloid stem cells. Thus, we investigated whether IL-3 accounts for this activity. Therefore, human thymic epithelial cells (TEC), fibroblasts, and adherent cells were isolated, and their culture supernatants assayed for myeloid colony promotion. Only supernatants from thymic epithelial cells supported colony-forming unit growth in semisolid media. This effect decreased following anti-IL-3 monoclonal antibody addition to these cultures. Furthermore, in situ hybridization showed the presence of IL-3 mRNA in epithelial cells. Effect of TEC culture conditions on IL-3 production by these cells was also studied. Together, these data show that IL-3 production is not the exclusive property of human activated T lymphocytes.

In Situ Forming, Dual-Crosslink Network, Self-Healing Hydrogel Enabled by a Bioorthogonal Nopoldiol–Benzoxaborolate Click Reaction with a Wide pH Range

2019 ◽  
Vol 31 (11) ◽  
pp. 4092-4102 ◽  
Author(s):  
Di Wu ◽  
Wenda Wang ◽  
Diana Diaz-Dussan ◽  
Yi-Yang Peng ◽  
Yangjun Chen ◽  
...  
Keyword(s):  
Click Reaction ◽  
Self Healing ◽  
In Situ Forming ◽  
Wide Ph Range ◽  
Ph Range

Altered thymic epithelial cells may be decisive for Moloney virus-induced lymphoma development

1983 ◽  
Vol 41 ◽  
pp. 784-785
Author(s):  
U.I. Heine ◽  
G.R.F. Krueger ◽  
E. Munoz ◽  
A. Karpinski
Keyword(s):  
Epithelial Cells ◽  
Leukemia Virus ◽  
Light And Electron Microscopy ◽  
Tumor Development ◽  
Current Evidence ◽  
Thymic Epithelial Cells ◽  
Thymic Tissue ◽  
Newborn Mice ◽  
Moloney Leukemia Virus ◽  
Differentiation Block

Infection of newborn mice with Moloney leukemia virus (M-MuLV) causes a T-cell differentiation block in the thymic cortex accompanied by proliferation and accumulation of prethymic lymphoblasts in the thymus and subsequent spreading of these cells to generate systemic lymphoma. Current evidence shows that thymic reticular epithelial cells (REC) provide a microenvironment necessary for the maturation of prethymic lymphoblasts to mature T-lymphocytes by secretion of various thymic factors. A change in that environment due to infection of REC by virus could be decisive for the failure of lymphoblasts to mature and thus contribute to lymphoma development.We have studied the morphology and distribution of the major thymic cell populations at different stages of tumorigenesis in Balb/c mice infected when newborn with 0.2ml M-MuLV suspension, 6.8 log FFU/ml. Thymic tissue taken at 1-2 weekly intervals up to tumor development was processed for light and electron microscopy, using glutaraldehyde-OsO4fixation and Epon-Araldite embedding.

Human thymic epithelial cells maintain long-term survival of clonogenic myeloid and erythroid progenitor cells in vitro

2000 ◽  
Vol 111 (1) ◽  
pp. 363-370 ◽  
Author(s):  
Katsuto Takenaka ◽  
Mine Harada ◽  
Tomoaki Fujisaki ◽  
Koji Nagafuji ◽  
Shinichi Mizuno ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Progenitor Cells ◽  
Thymic Epithelial Cells ◽  
Erythroid Progenitor ◽  
Term Survival ◽  
Long Term Survival ◽  
Erythroid Progenitor Cells
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