Electrostatic Forces and Dielectric Polarizability of Multiply Protonated Gas-Phase Cytochrome c Ions Probed by Ion/Molecule Chemistry

1995 ◽  
Vol 117 (25) ◽  
pp. 6747-6757 ◽  
Author(s):  
Paul D. Schnier ◽  
Deborah S. Gross ◽  
Evan R. Williams
Keyword(s):  
Cytochrome C ◽  
Gas Phase ◽  
Electrostatic Forces ◽  
Dielectric Polarizability

scholarly journals Evidence for unfolding and refolding of gas-phase cytochrome c ions in a Paul trap

2005 ◽  
Vol 16 (9) ◽  
pp. 1493-1497 ◽  
Author(s):  
Ethan R. Badman ◽  
Sunnie Myung ◽  
David E. Clemmer
Keyword(s):  
Cytochrome C ◽  
Gas Phase ◽  
Paul Trap

scholarly journals Gas Phase Stability of Protein Ions in a Cyclic Ion Mobility Spectrometry Travelling Wave Device

2018 ◽  
Author(s):  
Charles Eldrid ◽  
Jakub Ujma ◽  
Symeon Kalfas ◽  
nick tomczyk ◽  
Kevin Giles ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Gas Phase ◽  
Ion Mobility ◽  
Structural Changes ◽  
Structural Information ◽  
Travelling Wave ◽  
Resolving Power ◽  
Small Proteins ◽  
Multimeric Complex ◽  
Protein Ions

<div>Ion mobility mass spectrometry (IM-MS) allows separation of native protein ions into “conformational families”. Increasing the IM resolving power should allow finer structural information to be obtained, and can be achieved by increasing the length of the IM separator. This, however, increases the time that protein ions spend in the gas phase and previous experiments have shown that the initial conformations of small proteins can be lost within tens of milliseconds. Here, we report on investigations of protein ion stability using a multi-pass travelling wave (TW) cyclic IM (cIM) device. Using this device, minimal structural changes were observed for Cytochrome C after hundreds of milliseconds, while no changes were observed for a larger multimeric complex (Concanavalin A). The geometry of the instrument (Q-cIM-ToF) also enables complex tandem IM experiments to be performed which were used to obtain more detailed collision induced unfolding pathways for Cytochrome C. The novel instrument geometry provide unique capabilities with the potential to expand the field of protein analysis via IM-MS.</div>

scholarly journals Gas-phase folding and unfolding of cytochrome c cations.

1995 ◽  
Vol 92 (7) ◽  
pp. 2451-2454 ◽  
Author(s):  
T. D. Wood ◽  
R. A. Chorush ◽  
F. M. Wampler ◽  
D. P. Little ◽  
P. B. O'Connor ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Gas Phase

Naked Protein Conformations: Cytochrome c in the Gas Phase

1995 ◽  
Vol 117 (40) ◽  
pp. 10141-10142 ◽  
Author(s):  
David E. Clemmer ◽  
Robert R. Hudgins ◽  
Martin F. Jarrold
Keyword(s):  
Cytochrome C ◽  
Gas Phase ◽  
Protein Conformations

Measurement of Coulomb Energy and Dielectric Polarizability of Gas-Phase Diprotonated Diaminoalkanes

1995 ◽  
Vol 99 (12) ◽  
pp. 4034-4038 ◽  
Author(s):  
Deborah S. Gross ◽  
Sandra E. Rodriguez-Cruz ◽  
Sandra Bock ◽  
Evan R. Williams
Keyword(s):  
Gas Phase ◽  
Coulomb Energy ◽  
Dielectric Polarizability

The dynamics of water evaporation from partially solvated cytochrome c in the gas phase

2007 ◽  
Vol 9 (33) ◽  
pp. 4690 ◽  
Author(s):  
Michal Z. Steinberg ◽  
Kathrin Breuker ◽  
Ron Elber ◽  
R. Benny Gerber
Keyword(s):  
Cytochrome C ◽  
Gas Phase ◽  
Water Evaporation
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