The dynamics of water evaporation from partially solvated cytochrome c in the gas phase

2007 ◽  
Vol 9 (33) ◽  
pp. 4690 ◽  
Author(s):  
Michal Z. Steinberg ◽  
Kathrin Breuker ◽  
Ron Elber ◽  
R. Benny Gerber
Keyword(s):  
Cytochrome C ◽  
Gas Phase ◽  
Water Evaporation

scholarly journals A Comprehensive Review of the Cosmeceutical Benefits of Vanda Species (Orchidaceae)

2015 ◽  
Vol 10 (8) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Hazrina Hadi ◽  
Syarifah Nazira Said Razali ◽  
Ammar Ihsan Awadh
Keyword(s):  
Cytochrome C ◽  
Protein Expression ◽  
Cytochrome C Oxidase ◽  
Binding Capacity ◽  
Active Agent ◽  
Fold Increase ◽  
Skin Hydration ◽  
Water Evaporation ◽  
Structural Function ◽  
Water Binding

Orchidaceae is the largest family of flowering plants with over 35000 species and 850 genera. About 3300 species of orchids are found in Malaysia and the diversity is highest in the Main, Keledang, Bintang and Tahan Ranges. Apart from being prized for their beauty, orchids have long been used by humans for medicinal purposes. Today the uses of orchids have been expanded to the food and cosmetics industries. Many cosmeceutical companies use orchid extracts as an active ingredient in their products. Previous studies provide riveting insights into the potential uses of orchid extracts as an active agent in cosmetics. This paper describes the cosmeceutical potential of orchids as an anti-aging, and skin moisturizing agent. Orchid extracts from Vanda coerulea and V. teres delay aging caused by reactive oxygen species (ROS) following UV irradiation through their antioxidant and anti-inflammatory activity. These extracts also show anti-aging properties by stimulating cytochrome c oxidase (complex IV), which is part of the electron transport chain in mitochondria. Stimulation of cytochrome c oxidase improves the respiratory function of mitochondria in keratinocytes. The presence of mucilage in orchids enables them to maintain skin hydration. Mucilage functions as a moisturizer and emollient due to its high water binding capacity. Additionally, orchid extracts provide skin hydration by stimulating aquaporin 3 (AQP3) and LEKTI protein expression. The presence of AQP3 leads to a five-fold increase in water permeability, which subsequently increases stratum corneum hydration. Increased LEKTI protein expression mediated by orchid extracts reduces the degradation of desmoglein-1 and enhances the structural function of desmosomes, which play important roles in preventing water evaporation.

scholarly journals Evidence for unfolding and refolding of gas-phase cytochrome c ions in a Paul trap

2005 ◽  
Vol 16 (9) ◽  
pp. 1493-1497 ◽  
Author(s):  
Ethan R. Badman ◽  
Sunnie Myung ◽  
David E. Clemmer
Keyword(s):  
Cytochrome C ◽  
Gas Phase ◽  
Paul Trap

scholarly journals Gas Phase Stability of Protein Ions in a Cyclic Ion Mobility Spectrometry Travelling Wave Device

2018 ◽  
Author(s):  
Charles Eldrid ◽  
Jakub Ujma ◽  
Symeon Kalfas ◽  
nick tomczyk ◽  
Kevin Giles ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Gas Phase ◽  
Ion Mobility ◽  
Structural Changes ◽  
Structural Information ◽  
Travelling Wave ◽  
Resolving Power ◽  
Small Proteins ◽  
Multimeric Complex ◽  
Protein Ions

<div>Ion mobility mass spectrometry (IM-MS) allows separation of native protein ions into “conformational families”. Increasing the IM resolving power should allow finer structural information to be obtained, and can be achieved by increasing the length of the IM separator. This, however, increases the time that protein ions spend in the gas phase and previous experiments have shown that the initial conformations of small proteins can be lost within tens of milliseconds. Here, we report on investigations of protein ion stability using a multi-pass travelling wave (TW) cyclic IM (cIM) device. Using this device, minimal structural changes were observed for Cytochrome C after hundreds of milliseconds, while no changes were observed for a larger multimeric complex (Concanavalin A). The geometry of the instrument (Q-cIM-ToF) also enables complex tandem IM experiments to be performed which were used to obtain more detailed collision induced unfolding pathways for Cytochrome C. The novel instrument geometry provide unique capabilities with the potential to expand the field of protein analysis via IM-MS.</div>

scholarly journals Gas-phase folding and unfolding of cytochrome c cations.

1995 ◽  
Vol 92 (7) ◽  
pp. 2451-2454 ◽  
Author(s):  
T. D. Wood ◽  
R. A. Chorush ◽  
F. M. Wampler ◽  
D. P. Little ◽  
P. B. O'Connor ◽  
...  
Keyword(s):  
Cytochrome C ◽  
Gas Phase

Naked Protein Conformations: Cytochrome c in the Gas Phase

1995 ◽  
Vol 117 (40) ◽  
pp. 10141-10142 ◽  
Author(s):  
David E. Clemmer ◽  
Robert R. Hudgins ◽  
Martin F. Jarrold
Keyword(s):  
Cytochrome C ◽  
Gas Phase ◽  
Protein Conformations
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