A Kinetic Study of Concanavalin A Binding to Glycolipid Monolayers by Using a Quartz-Crystal Microbalance

1994 ◽  
Vol 116 (25) ◽  
pp. 11209-11212 ◽  
Author(s):  
Yasuhito Ebara ◽  
Yoshio Okahata
Polymers ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 1023
Author(s):  
Lili Guo ◽  
Shuang Chao ◽  
Pei Huang ◽  
Xiukai Lv ◽  
Quanquan Song ◽  
...  

A universal photochemical method to prepare carbohydrate sensors based on perfluorophenylazide (PFPA) modified polydopamine (PDA) for the study of carbohydrate–lectin interactions by a quartz crystal microbalance (QCM) biosensor was developed. The PFPA was immobilized on PDA-coated gold sensors via Schiff base reactions. Upon light irradiation, the underivatized carbohydrates were inserted into the sensor surface, including mannose, galactose, fucose and N-acetylglucosamine (GlcNAc). Carbohydrate sensors were evaluated for the binding to a series of plant lectins. A kinetic study of the interactions between mannose and concanavalin A (Con A), fucose and Ulex europaeus agglutinin I (UEA-I) were performed. This method can eliminate the tedious modification of carbohydrates, improve the experimental efficiency, and reduce the experimental cost, which is of great significance for the development of QCM biosensors and the study of biomolecular interactions.


2011 ◽  
Vol 13 (48) ◽  
pp. 21233 ◽  
Author(s):  
Gustavo B. Alcantara ◽  
Leonardo G. Paterno ◽  
André S. Afonso ◽  
Ronaldo C. Faria ◽  
Marcelo A. Pereira-da-Silva ◽  
...  

2009 ◽  
Vol 395 (2) ◽  
pp. 211-216 ◽  
Author(s):  
Toshiaki Mori ◽  
Momoko Toyoda ◽  
Tatsuro Ohtsuka ◽  
Yoshio Okahata

Author(s):  
Tippavan Hongkacharn ◽  
Verawat Champreda ◽  
Toemsak Srikhirin ◽  
Thidarat Wangkam ◽  
Tanakorn Osotchan

1999 ◽  
Vol 47 (7) ◽  
pp. 919-922 ◽  
Author(s):  
Kiminori KAWAKAMI ◽  
Masahiro YASUDA ◽  
Kazuhiro ISHII ◽  
Yoshio KOKUSENYA ◽  
Tadashi SATO

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