scholarly journals Concanavalin A–Polysaccharides binding affinity analysis using a quartz crystal microbalance

2014 ◽  
Vol 59 ◽  
pp. 404-411 ◽  
Author(s):  
Fohona S. Coulibaly ◽  
Bi-Botti C. Youan
Polymers ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 1023
Author(s):  
Lili Guo ◽  
Shuang Chao ◽  
Pei Huang ◽  
Xiukai Lv ◽  
Quanquan Song ◽  
...  

A universal photochemical method to prepare carbohydrate sensors based on perfluorophenylazide (PFPA) modified polydopamine (PDA) for the study of carbohydrate–lectin interactions by a quartz crystal microbalance (QCM) biosensor was developed. The PFPA was immobilized on PDA-coated gold sensors via Schiff base reactions. Upon light irradiation, the underivatized carbohydrates were inserted into the sensor surface, including mannose, galactose, fucose and N-acetylglucosamine (GlcNAc). Carbohydrate sensors were evaluated for the binding to a series of plant lectins. A kinetic study of the interactions between mannose and concanavalin A (Con A), fucose and Ulex europaeus agglutinin I (UEA-I) were performed. This method can eliminate the tedious modification of carbohydrates, improve the experimental efficiency, and reduce the experimental cost, which is of great significance for the development of QCM biosensors and the study of biomolecular interactions.


2009 ◽  
Vol 395 (2) ◽  
pp. 211-216 ◽  
Author(s):  
Toshiaki Mori ◽  
Momoko Toyoda ◽  
Tatsuro Ohtsuka ◽  
Yoshio Okahata

2019 ◽  
Vol 25 (6) ◽  
pp. 879-884
Author(s):  
Takahiro Sakai ◽  
Hayato Seki ◽  
Shogo Yoshida ◽  
Hayato Hori ◽  
Hisashi Suzuki ◽  
...  

1998 ◽  
Vol 145 (2) ◽  
pp. 492-497 ◽  
Author(s):  
James J. Kelly ◽  
K. M. Anisur Rahman ◽  
Christopher J. Durning ◽  
Alan C. West

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