Use of Selective Deuteration and 1H NMR in Demonstrating Major Groove Binding of .DELTA.-[Ru(phen)2dppz]2+ to d(GTCGAC)2

1994 ◽  
Vol 116 (22) ◽  
pp. 10286-10287 ◽  
Author(s):  
Cynthia M. Dupureur ◽  
Jacqueline K. Barton
Keyword(s):  
1H Nmr ◽  
Major Groove ◽  
Groove Binding ◽  
Selective Deuteration

scholarly journals 1H NMR Study of the Enantioselective Binding ofΛ- andΔ-[Ru(bpy)2(m-bpy-GHK)]Cl2to the Deoxynucleotide Duplex d(5'-C1G2C3G4A5A6T7T8C9G10C11G12-3')2

2005 ◽  
Vol 3 (1-2) ◽  
pp. 109-117 ◽  
Author(s):  
A. Myari ◽  
N. Hadjiliadis ◽  
A. Garoufis
Keyword(s):  
Nmr Spectroscopy ◽  
1H Nmr ◽  
Minor Groove ◽  
Major Groove ◽  
Nmr Analysis ◽  
Groove Binding ◽  
H Nmr ◽  
Nmr Study ◽  
Oligonucleotide Duplex ◽  
Diastereomeric Complexes

The interaction of the diastereomeric complexes Λ- andΔ-[Ru(bpy)2(m-GHK)]Cl2, (GHK = glycine-histidine-lysine) to the deoxynucleotide duplex d(5'-CGCGAATTCGCG-3')2was studied by means of1H NMR spectroscopy. The diastereomers interact with the oligonucleotide duplex differently. TheΔ-[Ru(bpy)2(m-GHK)]Cl2is characterized by major groove binding close to the central part of the oligonucleotide, with both the peptide and the bipyridine ligand of the complex involved in the binding. TheΛ-[Ru(bpy)2(m-bpy-GHK)]Cl2binds loosely, approaching the helix from the minor groove. The NMR analysis shows that the peptide (GHK) binding has a determinative role in the interactions of both diastereomers with the oligonucleotide.

scholarly journals 1H NMR study of [d(GCGATCGC)]2 and its interaction with minor groove binding 4',6-diamidino-2-phenylindole.

1993 ◽  
Vol 268 (6) ◽  
pp. 3944-3951
Author(s):  
E. Trotta ◽  
E. D'Ambrosio ◽  
N. Del Grosso ◽  
G. Ravagnan ◽  
M. Cirilli ◽  
...  
Keyword(s):  
1H Nmr ◽  
Minor Groove ◽  
Minor Groove Binding ◽  
Groove Binding ◽  
Nmr Study

scholarly journals Denaturation of dsDNA Induced by Specific Major Groove Binding of Cadmium Ion to Thymine

ACS Omega ◽  
2017 ◽  
Vol 2 (11) ◽  
pp. 8490-8494 ◽  
Author(s):  
Haiyang Gao ◽  
Zhi Zhu ◽  
Xiankai Jiang ◽  
Jun Guo ◽  
Bo Song ◽  
...  
Keyword(s):  
Major Groove ◽  
Groove Binding ◽  
Cadmium Ion

Mild and Green Protocol for Selective Deuteration of Quercetin-3-ORutinoside (Rutin) Under Aqueous Basic Conditions

2019 ◽  
Vol 6 (2) ◽  
pp. 147-151
Author(s):  
Anxhela Akolli ◽  
Silvia Moretti ◽  
Francesca Mangiavacchi ◽  
Bonifacio Monti ◽  
Claudio Santi
Keyword(s):  
1H Nmr ◽  
Deuterium Oxide ◽  
Cost Effective ◽  
Green Protocol ◽  
Mild Conditions ◽  
Cost Effective Method ◽  
Selective Deuteration

A convenient and cost-effective method for selective deuteration of rutin using biologically compatible bases and D2O both as a deuterium source and a solvent is herein reported. The protocol is benign and inexpensive affording good results in very mild conditions allowing to reduce the required amount of deuterium oxide. The position of the C-H/C-D exchange and the level of deuteration can be conveniently followed by 1H-NMR.

scholarly journals Naphthalene diimide bis-guanidinio-carbonyl-pyrrole as a pH-switchable threading DNA intercalator

2020 ◽  
Vol 16 ◽  
pp. 2201-2211
Author(s):  
Poulami Jana ◽  
Filip Šupljika ◽  
Carsten Schmuck ◽  
Ivo Piantanida
Keyword(s):  
Thermal Denaturation ◽  
Major Groove ◽  
Groove Binding ◽  
Dna Intercalator ◽  
Dna Minor Groove ◽  
Ph Values ◽  
Naphthalene Diimide ◽  
Threading Intercalation ◽  
Neutral Conditions ◽  
Pendant Arms

A novel naphthalene diimde analogue (NDI) equipped at the imide positions with two guanidinio-carbonyl-pyrrole (GCP) pendant arms interacted significantly stronger with ds-DNA at pH 5 than at pH 7, due to reversible protonation of the GCP arms. This was consequence of a pH-switchable threading intercalation into ds-DNAs only at pH 5, while at neutral conditions (pH 7) NDI-GCP2 switched to the DNA minor groove binding. Intriguingly, NDI-GCP2 was at both pH values studied bound to the ds-RNA major groove, still showing a higher affinity and thermal denaturation effect at pH 5 due to GCP protonation. At excess over the DNA/RNA conjugate NDI-GCP2 showed also aggregation along the ds-polynucleotide and AFM and DLS demonstrated that NDI-GCP2 has pronounced ds-DNA condensation ability.

NMR Evidence for Major Groove Binding of BothΛ- andΔ-[Ru(bpy)2(pqx)](PF6)2 to the Oligonucleotide d(CGCGAATTCGCG)2

2004 ◽  
Vol 2004 (16) ◽  
pp. 3326-3334 ◽  
Author(s):  
Achilleas Garoufis ◽  
Gerasimos Malandrinos ◽  
Nick Hadjiliadis
Keyword(s):  
Major Groove ◽  
Groove Binding

Major groove binding of the tRNA/mRNA complex to the 16 S ribosomal RNA decoding site 1 1Edited by D. E. Draper

1999 ◽  
Vol 285 (5) ◽  
pp. 2069-2078 ◽  
Author(s):  
Margaret S VanLoock ◽  
Thomas R Easterwood ◽  
Stephen C Harvey
Keyword(s):  
Ribosomal Rna ◽  
Major Groove ◽  
Groove Binding

scholarly journals Inhibition of major-groove-binding proteins by pyrrole-imidazole polyamides with an Arg-Pro-Arg positive patch

1998 ◽  
Vol 5 (3) ◽  
pp. 119-133 ◽  
Author(s):  
Ryan E. Bremer ◽  
Eldon E. Baird ◽  
Peter B. Dervan
Keyword(s):  
Binding Proteins ◽  
Major Groove ◽  
Groove Binding

Allosteric Inhibition of Zinc-Finger Binding in the Major Groove of DNA by Minor-Groove Binding Ligands†

Biochemistry ◽  
2004 ◽  
Vol 43 (13) ◽  
pp. 3880-3890 ◽  
Author(s):  
Doan H. Nguyen-Hackley ◽  
Elizabeth Ramm ◽  
Christina M. Taylor ◽  
J. Keith Joung ◽  
Peter B. Dervan ◽  
...  
Keyword(s):  
Zinc Finger ◽  
Minor Groove ◽  
Minor Groove Binding ◽  
Major Groove ◽  
Groove Binding ◽  
Allosteric Inhibition
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