Fabrication and imaging of two-dimensional patterns of proteins adsorbed on self-assembled monolayers by scanning electron microscopy

1993 ◽  
Vol 115 (23) ◽  
pp. 10774-10781 ◽  
Author(s):  
Gabriel P. Lopez ◽  
Hans A. Biebuyck ◽  
Ralph Harter ◽  
Amit Kumar ◽  
George M. Whitesides
Langmuir ◽  
1993 ◽  
Vol 9 (6) ◽  
pp. 1513-1516 ◽  
Author(s):  
Gabriel P. Lopez ◽  
Hans A. Biebuyck ◽  
George M. Whitesides

1999 ◽  
Vol 5 (6) ◽  
pp. 413-419 ◽  
Author(s):  
Bernardo R.A. Neves ◽  
Michael E. Salmon ◽  
Phillip E. Russell ◽  
E. Barry Troughton

Abstract: In this work, we show how field emission–scanning electron microscopy (FE-SEM) can be a useful tool for the study of self-assembled monolayer systems. We have carried out a comparative study using FE-SEM and atomic force microscopy (AFM) to assess the morphology and coverage of self-assembled monolayers (SAM) on different substrates. The results show that FE-SEM images present the same qualitative information obtained by AFM images when the SAM is deposited on a smooth substrate (e.g., mica). Further experiments with rough substrates (e.g., Al grains on glass) show that FE-SEM is capable of unambiguously identifying SAMs on any type of substrate, whereas AFM has significant difficulties in identifying SAMs on rough surfaces.


Langmuir ◽  
2011 ◽  
Vol 27 (10) ◽  
pp. 6235-6243 ◽  
Author(s):  
Benoit P. Pichon ◽  
Matthias Pauly ◽  
Pascal Marie ◽  
Cedric Leuvrey ◽  
Sylvie Begin-Colin

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