Rotational diffusion of Acridine Orange attached to SDS micelles

1989 ◽  
Vol 93 (22) ◽  
pp. 7694-7698 ◽  
Author(s):  
Shiow Hwa Chou ◽  
Mary J. Wirth
Keyword(s):  
Acridine Orange ◽  
Rotational Diffusion ◽  
Sds Micelles

scholarly journals THERMAL DEPOLARIZATION OF FLUORESCENCE FROM POLYTENE CHROMOSOMES STAINED WITH ACRIDINE ORANGE

1967 ◽  
Vol 33 (3) ◽  
pp. 597-604 ◽  
Author(s):  
James W. MacInnes ◽  
Robert B. Uretz
Keyword(s):  
Acridine Orange ◽  
Rotational Diffusion ◽  
Polytene Chromosomes ◽  
Degree Of Polarization ◽  
Fourth Chromosome ◽  
Thermal Change ◽  
Low Concentrations ◽  
Thermal Denaturation Temperature ◽  
Dna Thermal Denaturation ◽  
Increasing Temperature

The degree of polarization of fluorescence from stretched Chironomus thummi polytene chromosomes, stained with low concentrations of acridine orange (AO), decreases with increasing temperature. The "half temperature" of this decrease (T½R) is lower than the expected DNA thermal denaturation temperature (Tm) by about 20°C. T½R is lowered as histone is removed from chromosomes. Balbiani ring regions of the fourth chromosome have T½R's much lower than other regions, and nearly as low as chromosomes which had been extensively pretreated with trypsin to remove histone and other proteins. Measurements of the thermal change in the rotational diffusion rate of AO in solution with DNA indicate that the temperature at which the DNA-AO bonding changes from a "rigid" to a "loose" mode varies with the GC percentage of the DNA, and in the same fashion as Tm, although 20°C lower.

Vacuoles Induced in Mammalian Cells by Helicobacter Cytotoxin are Acidic Organelles

1990 ◽  
Vol 48 (3) ◽  
pp. 168-169
Author(s):  
M. H. Chestnut ◽  
C. E. Catrenich
Keyword(s):  
Acridine Orange ◽  
Mammalian Cells ◽  
Laser Scanning ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Ulcer Disease ◽  
Gastroduodenal Disease ◽  
H Pylori

Helicobacter pylori is a non-invasive, Gram-negative spiral bacterium first identified in 1983, and subsequently implicated in the pathogenesis of gastroduodenal disease including gastritis and peptic ulcer disease. Cytotoxic activity, manifested by intracytoplasmic vacuolation of mammalian cells in vitro, was identified in 55% of H. pylori strains examined. The vacuoles increase in number and size during extended incubation, resulting in vacuolar and cellular degeneration after 24 h to 48 h. Vacuolation of gastric epithelial cells is also observed in vivo during infection by H. pylori. A high molecular weight, heat labile protein is believed to be responsible for vacuolation and to significantly contribute to the development of gastroduodenal disease in humans. The mechanism by which the cytotoxin exerts its effect is unknown, as is the intracellular origin of the vacuolar membrane and contents. Acridine orange is a membrane-permeant weak base that initially accumulates in low-pH compartments. We have used acridine orange accumulation in conjunction with confocal laser scanning microscopy of toxin-treated cells to begin probing the nature and origin of these vacuoles.

scholarly journals Time-resolved detection of SDS-induced conformational changes in α-synuclein by a micro-stopped-flow system

RSC Advances ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 1086-1097
Author(s):  
Shunki Takaramoto ◽  
Yusuke Nakasone ◽  
Kei Sadakane ◽  
Shinsaku Maruta ◽  
Masahide Terazima
Keyword(s):  
Conformational Changes ◽  
Flow System ◽  
Stopped Flow ◽  
Time Resolved ◽  
Sds Micelles ◽  
Conformation Changes ◽  
System P

Dynamics of conformation changes of α-synuclein induced by the presence of SDS micelles are revealed using time-resolved diffusion, CD, and FRET measurements combined with a micro-stopped flow system.

scholarly journals A Turn-ON fluorometric biosensor based on ssDNA immobilized with a metal phenolic nanomaterial for the sequential detection of Pb(ii) and epirubicin cancer drug

RSC Advances ◽  
2021 ◽  
Vol 11 (20) ◽  
pp. 12361-12373
Author(s):  
A. Arunjegan ◽  
P. Rajaji ◽  
S. Sivanesan ◽  
P. Panneerselvam
Keyword(s):  
Acridine Orange ◽  
Cancer Drug ◽  
Sequential Detection ◽  
Label Free ◽  
Fluorescent Biosensor ◽  
Turn On

In this paper, we propose a fluorescent biosensor for the sequential detection of Pb2+ ions and the cancer drug epirubicin (Epn) using the interactions between label-free guanine-rich ssDNA (LFGr-ssDNA), acridine orange (AO), and a metal–phenolic nanomaterial.

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