Investigation of the micropolarities in reverse micelles of Triton X-100 in mixed solvents of benzene and n-hexane

1992 ◽  
Vol 96 (17) ◽  
pp. 7121-7126 ◽  
Author(s):  
D. M. Zhu ◽  
X. Wu ◽  
Z. A. Schelly
Keyword(s):  
Reverse Micelles ◽  
Mixed Solvents ◽  
Triton X

scholarly journals Formose reaction accelerated in aerosol-OT reverse micelles

2016 ◽  
Vol 12 ◽  
pp. 2663-2667 ◽  
Author(s):  
Makoto Masaoka ◽  
Tomohiro Michitaka ◽  
Akihito Hashidzume
Keyword(s):  
Reverse Micelles ◽  
Water Layer ◽  
Major Product ◽  
Hexadecyltrimethylammonium Bromide ◽  
Interfacial Water ◽  
Formose Reaction ◽  
Aerosol Ot ◽  
Aot Reverse Micelles ◽  
Triton X ◽  
C Nmr

The formose reaction in reverse micelles of aerosol-OT (AOT), triton X-100 (TX), and hexadecyltrimethylammonium bromide (CTAB) was investigated. Time–conversion data have indicated that the interfacial water layer of AOT reverse micelles is a medium that accelerates formation of glycolaldehyde in the formose reaction. The 13C NMR spectra for the products of the formose reaction using formaldehyde-13C as starting material are indicative of the formation of ethylene glycol as a major product.

Polarity profiles in reverse micelles of Triton X-100, as studied by spin probe and absorption probe techniques

1997 ◽  
Vol 72 (1-3) ◽  
pp. 105-119 ◽  
Author(s):  
Agneta Caragheorgheopol ◽  
Rodica Bandula ◽  
H. Caldararu ◽  
H. Joela
Keyword(s):  
Reverse Micelles ◽  
Spin Probe ◽  
Triton X

scholarly journals Optimization and in Silico Analysis of a Cold-Adapted Lipase from an Antarctic Pseudomonas sp. Strain AMS8 Reaction in Triton X-100 Reverse Micelles

Catalysts ◽  
2018 ◽  
Vol 8 (7) ◽  
pp. 289 ◽  
Author(s):  
Fatin Abd. Jalil ◽  
Raja Raja Abd. Rahman ◽  
Abu Salleh ◽  
Mohd Mohamad Ali
Keyword(s):  
In Silico ◽  
Reverse Micelles ◽  
Catalytic Domain ◽  
In Silico Analysis ◽  
Downstream Processing ◽  
Cold Adapted ◽  
Sodium Phosphate Buffer ◽  
Purification Technique ◽  
Purification Methods ◽  
Triton X

A moderate yield of a purified enzyme can be achieved by using the simple technique of reverse micellar extraction (RME). RME is a liquid–liquid extraction method that uses a surfactant and an organic solvent to extract biomolecules. Instead of traditional chromatographic purification methods, which are tedious and expensive, RME using the nonionic surfactant Triton X-100 and toluene is used as an alternative purification technique to purify a recombinant cold-adapted lipase, AMS8. Various process parameters were optimized to maximize the activity recovery of the AMS8 lipase. The optimal conditions were found to be 50 mM sodium phosphate buffer, pH 7, 0.125 M NaCl, and 0.07 M Triton X-100 in toluene at 10 °C. Approximately 56% of the lipase activity was successfully recovered. Structural analysis of the lipase in a reverse micelle (RM) was performed using an in silico approach. The predicted model of AMS8 lipase was simulated in the Triton X-100/toluene reverse micelles from 5 to 40 °C. The lid 2 was slightly opened at 10 °C. However, the secondary structure of AMS8 was most affected in the non-catalytic domain compared to the catalytic domain, with an increased coil conformation. These results suggest that an AMS8 lipase can be extracted using Triton X-100/water/toluene micelles at low temperature. This RME approach will be an important tool for the downstream processing of recombinant cold-adapted lipases.

Preparation of inorganic salts (CaCO3, BaCO3, CaSO4) nanowires in the Triton X-100/cyclohexane/water reverse micelles

2002 ◽  
Vol 244 (3-4) ◽  
pp. 379-383 ◽  
Author(s):  
Daibin Kuang ◽  
Anwu Xu ◽  
Yueping Fang ◽  
Huangdong Ou ◽  
Hanqin Liu
Keyword(s):  
Reverse Micelles ◽  
Inorganic Salts ◽  
Triton X

Energy transfer from C-phycocyanin to chlorophyll a in Triton X-100 reverse micelles

1998 ◽  
Vol 11 (1-2) ◽  
pp. 9-14 ◽  
Author(s):  
Zhao Jiquan ◽  
Zhao Jingquan ◽  
Xie Jie ◽  
Zhang Jianping ◽  
Jiang Lijin
Keyword(s):  
Energy Transfer ◽  
Chlorophyll A ◽  
Reverse Micelles ◽  
Triton X

scholarly journals Conductivity of AOT/Triton X-100 Mixed Reverse Micelles

2010 ◽  
Vol 26 (08) ◽  
pp. 2205-2210 ◽  
Author(s):  
HU Jia-Qi ◽  
◽  
KUANG Ya-Fei ◽  
ZHOU Hai-Hui ◽  
SUN Lu ◽  
...  
Keyword(s):  
Reverse Micelles ◽  
Triton X
Sign in / Sign up

Export Citation Format

Share Document