Metal Loading Capacity of Aβ N-Terminus: a Combined Potentiometric and Spectroscopic Study of Zinc(II) Complexes with Aβ(1−16), Its Short or Mutated Peptide Fragments and Its Polyethylene Glycol−ylated Analogue

2009 ◽  
Vol 48 (21) ◽  
pp. 10405-10415 ◽  
Author(s):  
Chiara A. Damante ◽  
Katalin Ősz ◽  
Zoltán Nagy ◽  
Giuseppe Pappalardo ◽  
Giulia Grasso ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Spectroscopic Study ◽  
Loading Capacity ◽  
Peptide Fragments ◽  
Metal Loading ◽  
N Terminus

Fabrication of polyethylene glycol hydrogels with enhanced swelling; loading capacity and release kinetics

2021 ◽  
Author(s):  
Qandeel Zahra ◽  
Muhammad Usman Minhas ◽  
Samiullah Khan ◽  
Pao-Chu Wu ◽  
Muhammad Suhail ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Release Kinetics ◽  
Loading Capacity

scholarly journals Copper(II) Coordination Abilities of the Tau Protein's N‐Terminus Peptide Fragments: A Combined Potentiometric, Spectroscopic and Mass Spectrometric Study

ChemPlusChem ◽  
2019 ◽  
Vol 84 (11) ◽  
pp. 1697-1708 ◽  
Author(s):  
Márton Lukács ◽  
Györgyi Szunyog ◽  
Ágnes Grenács ◽  
Norbert Lihi ◽  
Csilla Kállay ◽  
...  
Keyword(s):  
Mass Spectrometric Study ◽  
Mass Spectrometric ◽  
Spectrometric Study ◽  
Peptide Fragments ◽  
N Terminus

In vitrometabolism of an insect neuropeptide by homogenates of the nematodeCaenorhabditis elegans

2003 ◽  
Vol 77 (1) ◽  
pp. 43-48 ◽  
Author(s):  
E.P. Masler
Keyword(s):  
General Pattern ◽  
Residue Analysis ◽  
Aminopeptidase Activity ◽  
Soil Nematode ◽  
Peptide Fragments ◽  
Adipokinetic Hormone ◽  
Initial Product ◽  
Insect Neuropeptide ◽  
Endoproteolytic Cleavage ◽  
N Terminus

AbstractThe cytosolic fraction of homogenates from the free-living soil nematodeCaenorhabditis elegansis capable of metabolizing the insect neuropeptide adipokinetic hormone, a decapeptide blocked at the N-terminus by a pGlu residue. Analysis of digests by RP-HPLC and LC-MS revealed that an initial endoproteolytic cleavage step produced a heptapeptide with an unblocked N-terminus that can serve as a substrate for aminopeptidases. The aminopeptidase activity is depressed in the presence of the inhibitor amastatin; the initial product of the endoproteolytic step accumulates during incubation, and expected aminopeptidase product peptides are reduced in amount, as assessed by chromatographic peak size. The absence of some expected peptide fragments in the reaction mixtures suggests that multiple proteases contribute to short peptide half-lives. Comparison of the adipokinetic hormone digestion inC. elegansto that reported previously for insects reveals the same general pattern of peptide fragment production.

Water soluble multiarm-polyethylene glycol–betulinic acid prodrugs: design, synthesis, and in vivo effectiveness

2014 ◽  
Vol 5 (19) ◽  
pp. 5775-5783 ◽  
Author(s):  
Lin Dai ◽  
Dan Li ◽  
Jing Cheng ◽  
Jing Liu ◽  
Li-Hong Deng ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Betulinic Acid ◽  
Water Solubility ◽  
Drug Loading ◽  
Water Soluble ◽  
Loading Capacity ◽  
Design Synthesis ◽  
High Drug ◽  
High Drug Loading

Multiarm-polyethylene glycol–betulinic acid prodrugs were prepared by using multiarm-polyethylene glycol linkers and betulinic acid, which exhibited high drug loading capacity, good water solubility, and excellent anticancer activity.

scholarly journals Poloxamine/D-α-Tocopheryl polyethylene glycol succinate (TPGS) mixed micelles and gels: Morphology, loading capacity and skin drug permeability

2021 ◽  
Vol 324 ◽  
pp. 114930
Author(s):  
Joan Puig-Rigall ◽  
María J. Blanco-Prieto ◽  
Carlos Aydillo ◽  
Aurel Radulescu ◽  
Dolores Molero-Vilchez ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Mixed Micelles ◽  
Glycol Succinate ◽  
Loading Capacity ◽  
Drug Permeability

scholarly journals Electron-Based Dissociation Is Needed for O-Glycopeptides Derived from OpeRATOR Proteolysis

2020 ◽  
Author(s):  
Nicholas Riley ◽  
Stacy Malaker ◽  
Carolyn Bertozzi
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Tandem Mass ◽  
Peptide Fragments ◽  
Terminal Residue ◽  
Bacterial Enzyme ◽  
N Terminus ◽  
Modified Peptides ◽  
Potential Benefits

<p>The recently described O-glycoprotease OpeRATOR presents exciting opportunities for O-glycoproteomics. This bacterial enzyme purified from <i>Akkermansia (Sp). muciniphila</i> cleaves N-terminally to serine and threonine residues that are modified with (preferably asialylated) O-glycans. This <a>provides orthogonal cleavage relative to canonical proteases (e.g., trypsin) for improved O-glycopeptide characterization with tandem mass spectrometry (MS/MS). O-glycopeptides with a modified N-terminal residue, such as those generated by OpeRATOR, present several potential benefits, perhaps the most notable being <i>de facto</i> O-glycosite localization without the need of glycan-retaining fragments in MS/MS spectra. Indeed, O-glycopeptides modified exclusively at the N-terminus would enable O-glycoproteomic methods to rely solely on collision-based fragmentation rather than electron-driven dissociation because glycan-retaining peptide fragments would not be required for localization. The caveat is that modified peptides would need to reliably contain only a single O-glycosite. </a>Here we use methods that combine collision- and electron-based fragmentation to characterize the number of <i>O-</i>glycosites that are present in <i>O-</i>glycopeptides derived from OpeRATOR digestion of four known <i>O-</i>glycoproteins. Our data show that over 50% of <i>O-</i>glycopeptides generated from combined digestion using OpeRATOR and trypsin contain multiple <i>O-</i>glycosites, indicating that collision-based fragmentation alone is not sufficient. Electron-based dissociation methods are necessary to capture the <i>O-</i>glycopeptide diversity present in OpeRATOR digestions. </p>

A potentiometric and spectroscopic study of the interaction of angiotensin II and some of its peptide fragments with copper(II)

1988 ◽  
pp. 887 ◽  
Author(s):  
Brigitte Decock-Le Reverend ◽  
Ferid Liman ◽  
Cynara Livera ◽  
Leslie D. Pettit ◽  
Simon Pyburn ◽  
...  
Keyword(s):  
Angiotensin Ii ◽  
Spectroscopic Study ◽  
Peptide Fragments
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