scholarly journals Targeted Guanine Oxidation by a Dinuclear Copper(II) Complex at Single-Stranded/Double-Stranded DNA Junctions.

2006 ◽  
Vol 45 (22) ◽  
pp. 9145-9145
Author(s):  
Lei Li ◽  
Narasimha N. Murthy ◽  
Joshua Telser ◽  
Lev N. Zakharov ◽  
Glenn P. A. Yap ◽  
...  
Keyword(s):  
Guanine Oxidation ◽  
Dinuclear Copper ◽  
Double Stranded Dna ◽  
Dna Junctions

Targeted Guanine Oxidation by a Dinuclear Copper(II) Complex at Single Stranded/Double Stranded DNA Junctions

2006 ◽  
Vol 45 (18) ◽  
pp. 7144-7159 ◽  
Author(s):  
Lei Li ◽  
Narasimha N. Murthy ◽  
Joshua Telser ◽  
Lev. N. Zakharov ◽  
Glenn P. A. Yap ◽  
...  
Keyword(s):  
Guanine Oxidation ◽  
Dinuclear Copper ◽  
Double Stranded Dna ◽  
Dna Junctions

scholarly journals Analysis of Guanine Oxidation Products in Double-Stranded DNA and Proposed Guanine Oxidation Pathways in Single-Stranded, Double-Stranded or Quadruplex DNA

Biomolecules ◽  
2014 ◽  
Vol 4 (1) ◽  
pp. 140-159 ◽  
Author(s):  
Masayuki Morikawa ◽  
Katsuhito Kino ◽  
Takanori Oyoshi ◽  
Masayo Suzuki ◽  
Takanobu Kobayashi ◽  
...  
Keyword(s):  
Oxidation Products ◽  
Guanine Oxidation ◽  
Quadruplex Dna ◽  
Double Stranded Dna

scholarly journals Guanine Oxidation in Double-stranded DNA by MnTMPyP/KHSO5: At Least Three Independent Reaction Pathways

2001 ◽  
Vol 8 (1) ◽  
pp. 47-56 ◽  
Author(s):  
Andrea Lapi ◽  
Geneviève Pratviel ◽  
Bernard Meunier
Keyword(s):  
Degradation Products ◽  
Oxidation Products ◽  
Spiro Compound ◽  
Reaction Pathways ◽  
Guanine Oxidation ◽  
Double Stranded Dna ◽  
Independent Reaction ◽  
Reaction Routes ◽  
Damaged Dna

In order to better define the mechanism and the products of guanine oxidation within DNA, we investigated the details of the mechanism of guanine oxidation by a metalloporphyrin, Mn-TMPyP, associated to KHSO5 on oligonucleotides. We found that the three major products of guanine oxidation are formed by independent reaction routes. The oxidized guanidinohydantoin (1) and the proposed spiro compound 3 derivatives are not precursors of imidazolone lesion (Iz). These guanine lesions as well as their degradation products, may account for non-detected guanine oxidation products on oxidatively damaged DNA.

scholarly journals Electrochemical study of dsDNA on carbon nanotubes paste electrodes applying cyclic and differential pulse voltammetry

2013 ◽  
Vol 11 (3) ◽  
pp. 413-423 ◽  
Author(s):  
Constantina Serpi ◽  
Anastasios Voulgaropoulos ◽  
Stella Girousi
Keyword(s):  
Carbon Nanotubes ◽  
Stripping Voltammetry ◽  
Differential Pulse ◽  
Carbon Paste ◽  
Guanine Oxidation ◽  
Double Stranded Dna ◽  
Calf Thymus ◽  
Multi Walled Carbon Nanotubes ◽  
Pre Treatment ◽  
Walled Carbon Nanotubes

AbstractAbstract Carbon nanotubes paste electrodes (CNTPEs) in combination with adsorptive transfer stripping voltammetry are shown to be very suitable for the determination of calf thymus double-stranded DNA (dsDNA). The performance of three types of multi-walled carbon nanotubes paste electrodes (MWCNTPEs) is investigated. The effects of surface pre-treatment and accumulation conditions on the adsorption and electrooxidation of the dsDNA at MWCNTPEs are also described. The results indicate that the electroactivity inherent to carbon nanotubes/paste electrodes allows a large enhancement of the guanine oxidation signal compared to that obtained at the conventional carbon paste electrodes (CPEs). Moreover, the extent of the enhancement dependents on the type of MWCNTs incorporated into the paste. Based on the signal of guanine, under optimal conditions, very low levels of dsDNA can be detected following short accumulation times for all three types of MWCNTPEs (MWCNTPE1, MWCNTPE2, MWCNTPE3), with detection limits of 2.64 mg L−1, 2.02 mg L−1 and 1.46 mg L−1, respectively. Additionally, the dsDNA isolated from rat liver tissues is determined by use of the previously mentioned MWCNTPEs. Graphical abstract

DNA structure-dependent recruitment of telomeric proteins to single-stranded/double-stranded DNA junctions

2005 ◽  
Vol 328 (1) ◽  
pp. 49-56 ◽  
Author(s):  
Giscard H. Yanez ◽  
Sheik J. Khan ◽  
Alexandra M. Locovei ◽  
Ilene M. Pedroso ◽  
Terace M. Fletcher
Keyword(s):  
Dna Structure ◽  
Double Stranded Dna ◽  
Dna Junctions

scholarly journals Structure and function of a novel endonuclease acting on branched DNA substrates

2011 ◽  
Vol 39 (1) ◽  
pp. 145-149 ◽  
Author(s):  
Christophe Creze ◽  
Roxane Lestini ◽  
Joelle Kühn ◽  
Alessio Ligabue ◽  
Hubert F. Becker ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Nuclease Activity ◽  
Nuclear Antigen ◽  
Double Stranded Dna ◽  
Dna Junctions ◽  
Branched Dna ◽  
And Function ◽  
Proliferating Cell ◽  
Pyrococcus Abyssi

Branched DNA structures that occur during DNA repair and recombination must be efficiently processed by structure-specific endonucleases in order to avoid cell death. In the present paper, we summarize our screen for new interaction partners for the archaeal replication clamp that led to the functional characterization of a novel endonuclease family, dubbed NucS. Structural analyses of Pyrococcus abyssi NucS revealed an unexpected binding site for ssDNA (single-stranded DNA) that directs, together with the replication clamp, the nuclease activity of this protein towards ssDNA–dsDNA (double-stranded DNA) junctions. Our studies suggest that understanding the detailed architecture and dynamic behaviour of the NucS (nuclease specific for ssDNA)–PCNA (proliferating-cell nuclear antigen) complex with DNA will be crucial for identification of its physiologically relevant activities.

Discrimination of monomer from multimer DNA disk-shaped toruses by freezeetch TEM

1984 ◽  
Vol 42 ◽  
pp. 210-211
Author(s):  
George C. Ruben ◽  
Kenneth A. Marx
Keyword(s):  
Biochemical Data ◽  
Dna Structures ◽  
Ice Surface ◽  
Double Stranded Dna ◽  
Data Support ◽  
Dna Organization ◽  
Trivalent Cations

In vitro collapse of DNA by trivalent cations like spermidine produces torus (donut) shaped DNA structures thought to have a DNA organization similar to certain double stranded DNA bacteriophage and viruses. This has prompted our studies of these structures using freeze-etch low Pt-C metal (9Å) replica TEM. With a variety of DNAs the TEM and biochemical data support a circumferential DNA winding model for hydrated DNA torus organization. Since toruses are almost invariably oriented nearly horizontal to the ice surface one of the most accessible parameters of a torus population is annulus (ring) thickness. We have tabulated this parameter for populations of both nicked, circular (Fig. 1: n=63) and linear (n=40: data not shown) ϕX-174 DNA toruses. In both cases, as can be noted in Fig. 1, there appears to be a compact grouping of toruses possessing smaller dimensions separated from a dispersed population possessing considerably larger dimensions.

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