Room-temperature isolation and characterization of Group VI pentacarbonyls in porous Vycor glass

1983 ◽  
Vol 22 (3) ◽  
pp. 573-574 ◽  
Author(s):  
Robert Simon ◽  
Harry D. Gafney ◽  
David L. Morse
Keyword(s):  
Room Temperature ◽  
Group Vi ◽  
Vycor Glass ◽  
Isolation And Characterization ◽  
Porous Vycor Glass

scholarly journals The small-scale isolation and characterization of adrenocorticotrophin

1969 ◽  
Vol 114 (3) ◽  
pp. 519-528 ◽  
Author(s):  
R. O. Hussa ◽  
T. Winnick ◽  
J. Landon
Keyword(s):  
Room Temperature ◽  
Polyacrylamide Gel Electrophoresis ◽  
Gel Filtration ◽  
Paper Electrophoresis ◽  
Small Scale ◽  
Acid Extraction ◽  
Isolation And Characterization ◽  
Acetic Acid Extraction ◽  
Cellulose Column

Several methods for isolating adrenocorticotrophin from small quantities of porcine and bovine pituitary tissue are compared. Initial extraction of the hormone by an acid–acetone technique was simpler and more efficient than one employing acetic acid extraction and ether precipitation. Subsequent purification procedures utilizing adsorption of the peptide on to oxycellulose realized the highest yields. CM-cellulose-column chromatography followed by Sephadex-gel filtration were suitable final steps for obtaining highly purified adrenocorticotrophin. The purity of the hormone was demonstrated by determining its amino acid composition, C-terminal analysis, polyacrylamide-gel electrophoresis, chymotrypsin digestion and paper electrophoresis and by radioimmunoassay and bioassay. Adrenocorticotrophin was found to be rapidly destroyed in intact and especially in homogenized glands kept at room temperature. At 4° the rate of destruction was less rapid and at −20° losses were minimal.

scholarly journals Isolation and characterization of β- and γ-caseins from horse milk

1982 ◽  
Vol 203 (1) ◽  
pp. 131-139 ◽  
Author(s):  
S Visser ◽  
R Jenness ◽  
R J Mullin
Keyword(s):  
Room Temperature ◽  
Sodium Dodecyl ◽  
Sulphate Group ◽  
Group Iii ◽  
Isolation And Characterization ◽  
Group I ◽  
Beta Casein ◽  
Group Ii ◽  
Phosphate Groups

Three groups of casein components were isolated from horse milk. Group I is almost insoluble at acid and neutral pH, and is rather heterogeneous on alkaline gels with or without sodium dodecyl sulphate. Group II shows strong similarity to beta-casein from other species, as concluded from its amino acid composition and its N- and C-terminal sequences. This group consists of five electrophoretically distinguishable forms, all containing ester phosphate groups but no carbohydrate. Group III is composed of C-terminal fragments of the beta-like (group II) fraction and probably arises from the action of a plasmin-like enzyme present in horse milk. It does not contain phosphate or carbohydrate. Homology of this group with bovine gamma-caseins is demonstrated. Both beta- and gamma-like caseins are more soluble at 4 degrees C than at room temperature.

Magnetic Properties of Nanoscale Iron Particles Photodeposited in Glass

1995 ◽  
Vol 405 ◽  
Author(s):  
D. Sunil ◽  
H. D. Gafney ◽  
C. Tsang ◽  
M. H. Rafailovich ◽  
J. Sokolov ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Magnetic Properties ◽  
Magnetization Curve ◽  
Room Temperature ◽  
Iron Particles ◽  
Vycor Glass ◽  
Porous Vycor Glass ◽  
Transmission Electron ◽  
Nanoscale Iron Particles

AbstractTransmission Electron Microscopy(TEM) and magnetization measurements were used to study the correlation between size of the iron particles precipitated in glass and their magnetic properties. The iron particles les s than 40 Å in diameter photodeposited in porous Vycor glass(PVG) show superparamagnetic behavior at room temperature. As the size of the particles increases and reaches about 100 Å in diameter, the magnetization curve shows minor hysteresis with a very large coercivity.

scholarly journals Isolation and characterization of a bacteriophage F20 virulent to Enterobacter aerogenes

2012 ◽  
Vol 93 (10) ◽  
pp. 2310-2314 ◽  
Author(s):  
Charitra Kumar Mishra ◽  
Tae Jin Choi ◽  
Sun Chul Kang
Keyword(s):  
Room Temperature ◽  
Enterobacter Aerogenes ◽  
Oral Solution ◽  
Isolation And Characterization ◽  
Animal Trials ◽  
Physico Chemical ◽  
The Family ◽  
Linear Dsdna ◽  
Ph Range

An aquatic phage, designated F20, was characterized and its physico-chemical characteristics studied. F20 was specifically virulent to only two strains of Enterobacter aerogenes (ATCC 13048 and the multi-drug-resistant strain K113) among other species tested (n = 15). It was classified in the family Siphoviridae of T1-like viruses and contained a linear dsDNA genome estimated to be 51.5 kbp enclosed by an isometric capsid of 50±2 nm in diameter and a tail of 150±3 nm in length. F20 was able to survive in a broad pH range between 4 and 11, showed potential for future animal trials using oral solution and resisted chloroform and ethanol. It exhibited remarkable stability between room temperature and 70 °C for up to 150 min, and even up to 6 months at room temperature. Knowledge of this phage belonging to the widespread T1-like viruses might be helpful for adopting therapeutic strategies against E. aerogenes.

Catalytic reduction of dinitrogen to ammonia at well-defined single metal sites

2005 ◽  
Vol 363 (1829) ◽  
pp. 959-969 ◽  
Author(s):  
Richard R Schrock
Keyword(s):  
Catalytic Reaction ◽  
Room Temperature ◽  
Catalytic Reduction ◽  
High Yield ◽  
X Ray ◽  
Isolation And Characterization ◽  
Proton Source ◽  
Molybdenum Catalysts

Dinitrogen (N 2 ) is reduced to ammonia at room temperature and 1 atm with molybdenum catalysts that contain tetradentate [HIPTN 3 N] 3− triamidoamine ligands {[HIPTN 3 N] 3− =[{3,5-(2,4,6- i -Pr 3 C 6 H 2 ) 2 C 6 H 3 NCH 2 CH 2 } 3 N] 3− , an example being [HIPTN 3 N]Mo(N 2 )} in heptane. Slow addition of the proton source ({2,6-lutidinium}{BAr′ 4 }; Ar′=3,5-(CF 3 ) 2 C 6 H 3 ) and reductant (decamethyl chromocene) assure a high yield of ammonia (63–65% in four turnovers) versus dihydrogen formation. Numerous X-ray studies, along with isolation and characterization of seven intermediates in the proposed catalytic reaction (under noncatalytic conditions), suggest that N 2 is being reduced at a sterically protected, single Mo centre that cycles between states Mo(III), Mo(IV), Mo(V) and Mo(VI).

AN INFRARED STUDY OF THE ADSORPTION OF BUTENES ON SURFACES OF POROUS VYCOR GLASS

1961 ◽  
Vol 39 (1) ◽  
pp. 42-60 ◽  
Author(s):  
L. H. Little ◽  
H. E. Klauser ◽  
C. H. Amberg
Keyword(s):  
Room Temperature ◽  
Acid Leaching ◽  
Hydroxyl Groups ◽  
Infrared Study ◽  
Vycor Glass ◽  
Surface Hydroxyl ◽  
Pure Silica ◽  
Porous Vycor Glass ◽  
Initial Adsorption ◽  
Low Coverage

The adsorption and reactions of the four butenes on porous Vycor glass at room temperature have been studied by infrared spectroscopy. Initial adsorption was rapid and was found to perturb the surface hydroxyl groups of the glass. In the case of the n-butenes rapid isomerization took place at room temperature. In addition to this the adsorbate spectra showed the occurrence of a slower reaction which led to an increase in saturated C—H groupings. C8 and higher compounds were shown to have formed; on outgassing at room temperature small amounts of cracking products were collected. The polymerization of isobutene was particularly rapid. Experiments at low coverage (θ ∼ 0.001–0.002) gave identical initial spectra for the n-butenes. These were discussed in terms of possible models for butene adsorption. A reduction in the alumina and zirconia content of the glass by acid leaching resulted in decreased rates of isomerization and polymerization, suggesting that one or both of these oxides are at least partially responsible for the activity of the glass. A pure silica aerogel was almost completely inactive. Quantitative measurements of the spectra of several monoolefins in solution were made for comparison with the surface species, in particular with respect to their molar intensities of absorption.

Characterization of intergranular cuprous oxide in polycrystalline YBa2Cu3O7-x

1988 ◽  
Vol 46 ◽  
pp. 880-881
Author(s):  
Bradley L. Thiel ◽  
Chan Han R. P. ◽  
Kurosky L. C. Hutter ◽  
I. A. Aksay ◽  
Mehmet Sarikaya
Keyword(s):  
Grain Boundary ◽  
Cuprous Oxide ◽  
Room Temperature ◽  
Boundary Phase ◽  
Spectroscopic Techniques ◽  
Transition Width ◽  
Practical Applications ◽  
Thin Foils ◽  
Superconducting Oxides

The identification of extraneous phases is important in understanding of high Tc superconducting oxides. The spectroscopic techniques commonly used in determining the origin of superconductivity (such as RAMAN, XPS, AES, and EXAFS) are surface-sensitive. Hence a grain boundary phase several nanometers thick could produce irrelevant spectroscopic results and cause erroneous conclusions. The intergranular phases present a major technological consideration for practical applications. In this communication we report the identification of a Cu2O grain boundary phase which forms during the sintering of YBa2Cu3O7-x (1:2:3 compound).Samples are prepared using a mixture of Y2O3. CuO, and BaO2 powders dispersed in ethanol for complete mixing. The pellets pressed at 20,000 psi are heated to 950°C at a rate of 5°C per min, held for 1 hr, and cooled at 1°C per min to room temperature. The samples show a Tc of 91K with a transition width of 2K. In order to prevent damage, a low temperature stage is used in milling to prepare thin foils which are then observed, using a liquid nitrogen holder, in a Philips 430T at 300 kV.

The isolation and characterization of 18 equine microsatellite loci, TKY272–TKY289

2000 ◽  
Vol 31 (2) ◽  
pp. 149-149 ◽  
Author(s):  
T Tozaki ◽  
H Kakoi ◽  
S Mashima ◽  
K Hirota ◽  
T Hasegawa ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Isolation And Characterization
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