scholarly journals Rapid Molecular Detection of Invasive Species in Ballast and Harbor Water by Integrating Environmental DNA and Light Transmission Spectroscopy

2015 ◽  
Vol 49 (7) ◽  
pp. 4113-4121 ◽  
Author(s):  
Scott P. Egan ◽  
Erin Grey ◽  
Brett Olds ◽  
Jeffery L. Feder ◽  
Steven T. Ruggiero ◽  
...  
Keyword(s):  
Invasive Species ◽  
Molecular Detection ◽  
Light Transmission ◽  
Environmental Dna ◽  
Transmission Spectroscopy

scholarly journals Rapid Invasive Species Detection by Combining Environmental DNA with Light Transmission Spectroscopy

2013 ◽  
Vol 6 (6) ◽  
pp. 402-409 ◽  
Author(s):  
Scott P. Egan ◽  
Matthew A. Barnes ◽  
Ching-Ting Hwang ◽  
Andrew R. Mahon ◽  
Jeffery L. Feder ◽  
...  
Keyword(s):  
Invasive Species ◽  
Light Transmission ◽  
Environmental Dna ◽  
Species Detection ◽  
Transmission Spectroscopy

scholarly journals DNA-based species detection capabilities using laser transmission spectroscopy

2013 ◽  
Vol 10 (78) ◽  
pp. 20120637 ◽  
Author(s):  
A. R. Mahon ◽  
M. A. Barnes ◽  
F. Li ◽  
S. P. Egan ◽  
C. E. Tanner ◽  
...  
Keyword(s):  
Invasive Species ◽  
Dna Detection ◽  
Field Application ◽  
Economic Damage ◽  
Oligonucleotide Probes ◽  
Target Species ◽  
Dreissena Bugensis ◽  
Species Detection ◽  
Transmission Spectroscopy ◽  
Species Specific

Early detection of invasive species is critical for effective biocontrol to mitigate potential ecological and economic damage. Laser transmission spectroscopy (LTS) is a powerful solution offering real-time, DNA-based species detection in the field. LTS can measure the size, shape and number of nanoparticles in a solution and was used here to detect size shifts resulting from hybridization of the polymerase chain reaction product to nanoparticles functionalized with species-specific oligonucleotide probes or with the species-specific oligonucleotide probes alone. We carried out a series of DNA detection experiments using the invasive freshwater quagga mussel ( Dreissena bugensis ) to evaluate the capability of the LTS platform for invasive species detection. Specifically, we tested LTS sensitivity to (i) DNA concentrations of a single target species, (ii) the presence of a target species within a mixed sample of other closely related species, (iii) species-specific functionalized nanoparticles versus species-specific oligonucleotide probes alone, and (iv) amplified DNA fragments versus unamplified genomic DNA. We demonstrate that LTS is a highly sensitive technique for rapid target species detection, with detection limits in the picomolar range, capable of successful identification in multispecies samples containing target and non-target species DNA. These results indicate that the LTS DNA detection platform will be useful for field application of target species. Additionally, we find that LTS detection is effective with species-specific oligonucleotide tags alone or when they are attached to polystyrene nanobeads and with both amplified and unamplified DNA, indicating that the technique may also have versatility for broader applications.

scholarly journals Use of environmental DNA (eDNA) in streams to detect feral swine (Sus scrofa)

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8287
Author(s):  
Amberly N. Hauger ◽  
Karmen M. Hollis-Etter ◽  
Dwayne R. Etter ◽  
Gary J. Roloff ◽  
Andrew R. Mahon
Keyword(s):  
Invasive Species ◽  
Water Temperature ◽  
Linear Model ◽  
Detection Probability ◽  
Generalized Linear Model ◽  
Environmental Dna ◽  
The United States ◽  
Stream Data ◽  
Feral Swine ◽  
Animal Populations

Invasive feral swine can damage ecosystems, disrupt plant and animal populations, and transmit diseases. Monitoring of feral swine populations requires expensive and labor-intensive techniques such as aerial surveys, field surveys for sign, trail cameras, and verifying landowner reports. Environmental DNA (eDNA) provides an alternative method for locating feral swine. To aid in detection of this harmful invasive species, a novel assay was developed incorporating molecular methods. From August 2017 to April 2018, water samples and stream data were collected along 400 m transects in two different stream types where swine DNA was artificially introduced to investigate potential factors affecting detection. A generalized linear model (family binomial) was used to characterize environmental conditions affecting swine DNA detection; detection was the dependent variable and stream measurements included stream type, distance downstream, water temperature, velocity, turbidity, discharge, and pH as independent variables. Parameters from the generalized linear model were deemed significant if 95% confidence intervals did not overlap 0. Detection probability for swine DNA negatively related to water temperature (β =  − 0.21, 95% CI [−0.35 to −0.09]), with the highest detection probability (0.80) at 0 °C and lowest detection probability (0.05) at 17.9 °C water temperature. Results indicate that sampling for swine eDNA in free-flowing stream systems should occur at lower water temperatures to maximize detection probability. This study provides a foundation for further development of field and sampling techniques for utilizing eDNA as a viable alternative to monitoring a terrestrial invasive species in northern regions of the United States.

Detection of a global aquatic invasive amphibian, Xenopus laevis, using environmental DNA

2016 ◽  
Vol 37 (1) ◽  
pp. 131-136 ◽  
Author(s):  
Jean Secondi ◽  
Tony Dejean ◽  
Alice Valentini ◽  
Benjamin Audebaud ◽  
Claude Miaud
Keyword(s):  
Invasive Species ◽  
Xenopus Laevis ◽  
Dna Amplification ◽  
Control Program ◽  
Environmental Dna ◽  
Climatic Conditions ◽  
Low Density ◽  
African Species ◽  
A Site ◽  
And Control

Detection is crucial in the study and control of invasive species but it may be limited by methodological issues. In amphibians, classical survey techniques exhibit variable detection probability depending on species and are often constrained by climatic conditions often requiring several site visits. Furthermore, detection may be reduced at low density because probability capture (passive traps), or activity (acoustic surveys) drop. Such limits may impair the study of invasive species because low density is typical of the onset of colonisation on a site. In the last few years, environmental DNA (eDNA) methods have proved their ability to detect the presence of aquatic species. We developed here an eDNA method to detectXenopus laevisin ponds. This austral African species is now present worldwide because of its use in biology and as a pet. Populations have settled and expanded on several continents so that it is now considered as one of the major invasive amphibians in the World. We detected the presence ofX. laevisat density as low as 1 ind/100 m2and found a positive relationship between density in ponds and rate of DNA amplification. Results show that eDNA can be successfully applied to survey invasive populations ofX. laeviseven at low density in order to confirm suspected cases of introduction, delimit the expansion of a colonized range, or monitor the efficiency of a control program.

Nanoparticle analysis of cancer cells by light transmission spectroscopy

2015 ◽  
Vol 484 ◽  
pp. 58-65 ◽  
Author(s):  
N. Sun ◽  
J. Johnson ◽  
M.S. Stack ◽  
J. Szajko ◽  
C. Sander ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Light Transmission ◽  
Transmission Spectroscopy

The use of environmental DNA in invasive species surveillance of the Great Lakes commercial bait trade

2014 ◽  
Vol 29 (2) ◽  
pp. 430-439 ◽  
Author(s):  
Lucas R. Nathan ◽  
Christopher L. Jerde ◽  
Michelle L. Budny ◽  
Andrew R. Mahon
Keyword(s):  
Invasive Species ◽  
Great Lakes ◽  
Environmental Dna

Are Environmental DNA Methods Ready for Aquatic Invasive Species Management?

2020 ◽  
Vol 35 (8) ◽  
pp. 668-678 ◽  
Author(s):  
Adam J. Sepulveda ◽  
Nanette M. Nelson ◽  
Christopher L. Jerde ◽  
Gordon Luikart
Keyword(s):  
Invasive Species ◽  
Environmental Dna ◽  
Aquatic Invasive Species ◽  
Invasive Species Management ◽  
Species Management

scholarly journals Detecting an elusive invasive species: a diagnostic PCR to detect Burmese python in Florida waters and an assessment of persistence of environmental DNA

2013 ◽  
Vol 14 (2) ◽  
pp. 374-380 ◽  
Author(s):  
Antoinette J. Piaggio ◽  
Richard M. Engeman ◽  
Matthew W. Hopken ◽  
John S. Humphrey ◽  
Kandy L. Keacher ◽  
...  
Keyword(s):  
Invasive Species ◽  
Environmental Dna ◽  
Diagnostic Pcr

scholarly journals Strategic considerations for invasive species managers in the utilization of environmental DNA (eDNA): steps for incorporating this powerful surveillance tool

2021 ◽  
Vol 12 (3) ◽  
pp. 747-775
Author(s):  
Jeffrey Morisette ◽  
Stanley Burgiel ◽  
Kelsey Brantley ◽  
Wesley Daniel ◽  
John Darling ◽  
...  
Keyword(s):  
Invasive Species ◽  
Environmental Dna
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