Nanoparticle analysis of cancer cells by light transmission spectroscopy

2015 ◽  
Vol 484 ◽  
pp. 58-65 ◽  
Author(s):  
N. Sun ◽  
J. Johnson ◽  
M.S. Stack ◽  
J. Szajko ◽  
C. Sander ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Light Transmission ◽  
Transmission Spectroscopy

scholarly journals Variations in Cell Surfaces of Estrogen Treated Breast Cancer Cells Detected by A Combined Instrument for Far-Field and Near-Field Microscopy

2002 ◽  
Vol 24 (2-3) ◽  
pp. 89-100 ◽  
Author(s):  
P. Perner ◽  
A. Rapp ◽  
C. Dressler ◽  
L. Wollweber ◽  
J. Beuthan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Surface Topography ◽  
Breast Cancer Cells ◽  
Light Transmission ◽  
Single Cells ◽  
Near Field ◽  
Far Field ◽  
Breast Cancers ◽  
General Behaviour

The response of single breast cancer cells (cell line T‐47D) to 17β‐estradiol (E2) under different concentrations was studied by using an instrument that allows to combine far‐field light microscopy with high resolution scanning near‐field (AFM/SNOM) microscopy on the same cell. Different concentrations of E2induce clearly different effects as well on cellular shape (in classical bright‐field imaging) as on surface topography (atomic force imaging) and absorbance (near‐field light transmission imaging). The differences range from a polygonal shape at zero via a roughly spherical shape at physiological up to a spindle‐like shape at un‐physiologically high concentrations. The surface topography of untreated control cells was found to be regular and smooth with small overall height modulations. At physiological E2concentrations the surfaces became increasingly jagged as detected by an increase in membrane height. After application of the un‐physiological high E2concentration the cell surface structures appeared to be smoother again with an irregular fine structure. The general behaviour of dose dependent differences was also found in the near‐field light transmission images. In order to quantify the treatment effects, line scans through the normalised topography images were drawn and a rate of co‐localisation between high topography and high transmission areas was calculated. The cell biological aspects of these observations are, so far, not studied in detail but measurements on single cells offer new perspectives to be empirically used in diagnosis and therapy control of breast cancers.

scholarly journals Synthesis and evaluation of cationic bacteriochlorin amphiphiles with effective in vitro photodynamic activity against cancer cells at low nanomolar concentration

2013 ◽  
Vol 17 (01n02) ◽  
pp. 73-85 ◽  
Author(s):  
Sulbha K. Sharma ◽  
Michael Krayer ◽  
Felipe F. Sperandio ◽  
Liyi Huang ◽  
Ying-Ying Huang ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Near Infrared ◽  
De Novo ◽  
Light Transmission ◽  
Human Cancer ◽  
Nuclear Condensation ◽  
Human Cancer Cells ◽  
Nanomolar Concentration ◽  
Single Positive

Bacteriochlorins are attractive candidates as photosensitizers for photodynamic therapy (PDT) due to their intense absorption in the near-infrared (NIR) region of the spectrum where light transmission through tissue is maximal. Many naturally occurring bacteriochlorins are inherently unstable due to adventitious atmospheric oxidation. A de novo synthesis affords bacteriochlorins that contain a geminal dimethyl group in each reduced pyrrole ring to increase stability against oxidation. Here, three new synthetic bacteriochlorins, each bearing a single side-chain containing one or two positive charges, were investigated for their in vitro PDT activity against HeLa human cancer cells. All bacteriochlorins were active at low nanomolar concentration when activated with NIR light; those bearing a single positive charge exhibited faster uptake and higher activity. The bacteriochlorins were localized in mitochondria, lysosomes and endoplasmic reticulum as shown by organelle specific fluorescent probes. Cell death was via apoptosis as shown by cell morphology and nuclear condensation. Taken together, the results show the importance of appropriate peripheral groups about a photosensitizer for effective PDT applications.

Light Transmission Spectroscopy

2019 ◽  
pp. 1-12
Author(s):  
Carol E. Tanner ◽  
Steven T. Ruggiero
Keyword(s):  
Light Transmission ◽  
Transmission Spectroscopy

Development of a Real-Time,In-SituParticle Sizing Technique: Real-Time Light Transmission Spectroscopy (RTLTS)

2013 ◽  
Vol 47 (10) ◽  
pp. 1092-1100 ◽  
Author(s):  
Jaehyuk Yoon ◽  
Sungsoo Park ◽  
Kwang Min Chun ◽  
Soonho Song
Keyword(s):  
Real Time ◽  
Light Transmission ◽  
Transmission Spectroscopy

scholarly journals Rapid Molecular Detection of Invasive Species in Ballast and Harbor Water by Integrating Environmental DNA and Light Transmission Spectroscopy

2015 ◽  
Vol 49 (7) ◽  
pp. 4113-4121 ◽  
Author(s):  
Scott P. Egan ◽  
Erin Grey ◽  
Brett Olds ◽  
Jeffery L. Feder ◽  
Steven T. Ruggiero ◽  
...  
Keyword(s):  
Invasive Species ◽  
Molecular Detection ◽  
Light Transmission ◽  
Environmental Dna ◽  
Transmission Spectroscopy

Rapid quantitative protein detection by light transmission spectroscopy

2019 ◽  
Vol 58 (4) ◽  
pp. 1121
Author(s):  
Alison E. Deatsch ◽  
Arial J. Shogren ◽  
Scott P. Egan ◽  
Jennifer L. Tank ◽  
Nan Sun ◽  
...  
Keyword(s):  
Light Transmission ◽  
Protein Detection ◽  
Transmission Spectroscopy

scholarly journals Rapid Invasive Species Detection by Combining Environmental DNA with Light Transmission Spectroscopy

2013 ◽  
Vol 6 (6) ◽  
pp. 402-409 ◽  
Author(s):  
Scott P. Egan ◽  
Matthew A. Barnes ◽  
Ching-Ting Hwang ◽  
Andrew R. Mahon ◽  
Jeffery L. Feder ◽  
...  
Keyword(s):  
Invasive Species ◽  
Light Transmission ◽  
Environmental Dna ◽  
Species Detection ◽  
Transmission Spectroscopy

Light transmission spectroscopy in real time: a high-resolution nanoparticle analysis instrument

2017 ◽  
Vol 56 (7) ◽  
pp. 1908 ◽  
Author(s):  
Carol E. Tanner ◽  
Nan Sun ◽  
Alison Deatsch ◽  
Frank Li ◽  
Steven T. Ruggiero
Keyword(s):  
High Resolution ◽  
Real Time ◽  
Light Transmission ◽  
Transmission Spectroscopy ◽  
Analysis Instrument
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