Accumulation and Embryotoxicity of Polystyrene Nanoparticles at Early Stage of Development of Sea Urchin Embryos Paracentrotus lividus

2014 ◽  
Vol 48 (20) ◽  
pp. 12302-12311 ◽  
Author(s):  
C. Della Torre ◽  
E. Bergami ◽  
A. Salvati ◽  
C. Faleri ◽  
P. Cirino ◽  
...  
1975 ◽  
Vol 42 (2-3) ◽  
pp. 201-204 ◽  
Author(s):  
Ida Pucci-Minafra ◽  
Salvatore Minafra ◽  
Fabrizio Gianguzza ◽  
Caterina Casano

1978 ◽  
Vol 174 (1) ◽  
pp. 95-102 ◽  
Author(s):  
E Di Mauro ◽  
F Pedone ◽  
M Pomponi

Non-histone chromosomal proteins prepared from chromosomal material of the sea-urchin Paracentrotus lividus affect RNA synthesis in vitro. 1. The extent of transcription can be radically changed from inhibition to stimulation, depending on the DNA/non-histone chromosomal proteins ratio. 2. A correlation exists between stage of development and influence on transcription. 3. Non-histone chromosomal proteins exert their action by intervening directly on some initiation step of RNA synthesis, as shown by the numbers of initiation events that take place in their presence or absence. 4. Stimulatory activity is observed only in restrictive conditions of ionic strength and temperature. These observations are in agreement with models that predict for non-histone chromosomal proteins a regulatory role on the transcription process exerted through a modulation of promoter availability.


2019 ◽  
Vol 6 (10) ◽  
pp. 2937-2947 ◽  
Author(s):  
Giacomo Grassi ◽  
Claudia Landi ◽  
Camilla Della Torre ◽  
Elisa Bergami ◽  
Luca Bini ◽  
...  

The biological identity of oppositely charged polystyrene nanoparticles entails the potential for cell interactions and toxicity.


1977 ◽  
Vol 33 (1) ◽  
pp. 38-39
Author(s):  
N. Wolfson ◽  
M. Acara ◽  
B. Rennick

2016 ◽  
Vol 18 (3) ◽  
pp. 384-395 ◽  
Author(s):  
Konstantinos Karakostis ◽  
Caterina Costa ◽  
Francesca Zito ◽  
Franz Brümmer ◽  
Valeria Matranga

1972 ◽  
Vol 55 (1) ◽  
pp. 74-81 ◽  
Author(s):  
Brian L. Kotzin ◽  
Robert F. Baker

Experimental evidence suggests that exposure of sea urchin gastrulae to 5-bromodeoxyuridine (BUdR), an analog of thymidine, causes a reduction in the rate of synthesis of some RNA species usually transcribed at this stage of development. In pulse-chase experiments, 3H (in gastrula stage 8–15S nuclear DNA labeled with BUdR-3H) could not be chased, with unlabeled BUdR, into 20–60S size-range DNA; in similar experiments in which gastrulae were pulsed with thymidine-3H and then chased with unlabeled thymidine, 3H in 8–15S nuclear DNA could be extensively chased into 20–60S DNA. DNA-RNA hybridization assays indicate that gastrula stage nuclear DNA in the range of 20–60S has greater sequence homology for gastrula stage RNA-3H than does nuclear DNA of similar size-range taken from gastrulae exposed to BUdR for 1 hr. An explanation is offered for the effect of BUdR on transcription and DNA replication in sea urchin embryos.


1974 ◽  
Vol 52 (12) ◽  
pp. 1531-1534
Author(s):  
N. Wolfson

Live embryos of the sea urchin, Paracentrotus lividus, release materials which decolor the yellow ion formed by the reagent DTNB (5,5′-dithiobis(2-nitrobenzoic acid)) in amounts titratable with dithiothreitol, and this decolorant activity increased to about the 32-cell stage, declining gradually thereafter. The embryos release decolorants into a saline medium only if it contains Tris buffer and little or no calcium and is adjusted to a pH below 8.


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