Microbial Phenazine Production Enhances Electron Transfer in Biofuel Cells

2005 ◽  
Vol 39 (9) ◽  
pp. 3401-3408 ◽  
Author(s):  
Korneel Rabaey ◽  
Nico Boon ◽  
Monica Höfte ◽  
Willy Verstraete
Keyword(s):  
Electron Transfer ◽  
Biofuel Cells ◽  
Phenazine Production

scholarly journals Strain- and Substrate-Dependent Redox Mediator and Electricity Production by Pseudomonas aeruginosa

2016 ◽  
Vol 82 (16) ◽  
pp. 5026-5038 ◽  
Author(s):  
Erick M. Bosire ◽  
Lars M. Blank ◽  
Miriam A. Rosenbaum
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Electron Transfer ◽  
Fuel Cells ◽  
Microbial Fuel Cells ◽  
Pure Culture ◽  
Bioelectrochemical Systems ◽  
Content Type ◽  
Mediated Electron Transfer ◽  
Phenazine Production ◽  
Model Strain

ABSTRACTPseudomonas aeruginosais an important, thriving member of microbial communities of microbial bioelectrochemical systems (BES) through the production of versatile phenazine redox mediators. Pure culture experiments with a model strain revealed synergistic interactions ofP. aeruginosawith fermenting microorganisms whereby the synergism was mediated through the shared fermentation product 2,3-butanediol. Our work here shows that the behavior and efficiency ofP. aeruginosain mediated current production is strongly dependent on the strain ofP. aeruginosa. We compared levels of phenazine production by the previously investigated model strainP. aeruginosaPA14, the alternative model strainP. aeruginosaPAO1, and the BES isolatePseudomonassp. strain KRP1 with glucose and the fermentation products 2,3-butanediol and ethanol as carbon substrates. We found significant differences in substrate-dependent phenazine production and resulting anodic current generation for the three strains, with the BES isolate KRP1 being overall the best current producer and showing the highest electrochemical activity with glucose as a substrate (19 μA cm−2with ∼150 μg ml−1phenazine carboxylic acid as a redox mediator). Surprisingly,P. aeruginosaPAO1 showed very low phenazine production and electrochemical activity under all tested conditions.IMPORTANCEMicrobial fuel cells and other microbial bioelectrochemical systems hold great promise for environmental technologies such as wastewater treatment and bioremediation. While there is much emphasis on the development of materials and devices to realize such systems, the investigation and a deeper understanding of the underlying microbiology and ecology are lagging behind. Physiological investigations focus on microorganisms exhibiting direct electron transfer in pure culture systems. Meanwhile, mediated electron transfer with natural redox compounds produced by, for example,Pseudomonas aeruginosamight enable an entire microbial community to access a solid electrode as an alternative electron acceptor. To better understand the ecological relationships between mediator producers and mediator utilizers, we here present a comparison of the phenazine-dependent electroactivities of threePseudomonasstrains. This work forms the foundation for more complex coculture investigations of mediated electron transfer in microbial fuel cells.

Electrochemical communication between microbial cells and electrodes via osmium redox systems

2012 ◽  
Vol 40 (6) ◽  
pp. 1330-1335 ◽  
Author(s):  
Kamrul Hasan ◽  
Sunil A. Patil ◽  
Dónal Leech ◽  
Cecilia Hägerhäll ◽  
Lo Gorton
Keyword(s):  
Electron Transfer ◽  
Biofuel Cells ◽  
Major Group ◽  
Electrochemical Biosensors ◽  
Bioelectrochemical Systems ◽  
Bacterial Cells ◽  
Redox Systems ◽  
Microbial Cells ◽  
Fundamental Part ◽  
Micro Organisms

Electrochemical communication between micro-organisms and electrodes is the integral and fundamental part of BESs (bioelectrochemical systems). The immobilization of bacterial cells on the electrode and ensuring efficient electron transfer to the electrode via a mediator are decisive features of mediated electrochemical biosensors. Notably, mediator-based systems are essential to extract electrons from the non-exoelectrogens, a major group of microbes in Nature. The advantage of using polymeric mediators over diffusible mediators led to the design of osmium redox polymers. Their successful use in enzyme-based biosensors and BFCs (biofuel cells) paved the way for exploring their use in microbial BESs. The present mini-review focuses on osmium-bound redox systems used to date in microbial BESs and their role in shuttling electrons from viable microbial cells to electrodes.

Materials for Mediator-Free Electron Transfer in the Enzymatic Electrodes of Biobatteries and Biofuel Cells

2013 ◽  
Vol 45 (11) ◽  
pp. 1-8 ◽  
Author(s):  
K. Zelechowska ◽  
K. Stolarczyk ◽  
D. Lyp ◽  
J. F. Biernat ◽  
J. Rogalski ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Free Electron ◽  
Biofuel Cells

scholarly journals Nanocatalysts Containing Direct Electron Transfer-Capable Oxidoreductases: Recent Advances and Applications

Catalysts ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 9 ◽  
Author(s):  
Dalius Ratautas ◽  
Marius Dagys
Keyword(s):  
Electron Transfer ◽  
State Of The Art ◽  
Direct Electron Transfer ◽  
Biofuel Cells ◽  
Solid Surfaces ◽  
Electron Mediator ◽  
Research Directions ◽  
Catalytic Systems ◽  
Nanoparticle Catalysts ◽  
Nanoparticle Surface

Direct electron transfer (DET)-capable oxidoreductases are enzymes that have the ability to transfer/receive electrons directly to/from solid surfaces or nanomaterials, bypassing the need for an additional electron mediator. More than 100 enzymes are known to be capable of working in DET conditions; however, to this day, DET-capable enzymes have been mainly used in designing biofuel cells and biosensors. The rapid advance in (semi) conductive nanomaterial development provided new possibilities to create enzyme-nanoparticle catalysts utilizing properties of DET-capable enzymes and demonstrating catalytic processes never observed before. Briefly, such nanocatalysts combine several cathodic and anodic catalysis performing oxidoreductases into a single nanoparticle surface. Hereby, to the best of our knowledge, we present the first review concerning such nanocatalytic systems involving DET-capable oxidoreductases. We outlook the contemporary applications of DET-capable enzymes, present a principle of operation of nanocatalysts based on DET-capable oxidoreductases, provide a review of state-of-the-art (nano) catalytic systems that have been demonstrated using DET-capable oxidoreductases, and highlight common strategies and challenges that are usually associated with those type catalytic systems. Finally, we end this paper with the concluding discussion, where we present future perspectives and possible research directions.

Development of Biofuel Cell Using a Complex of Highly Oriented Immobilized His-Tagged Enzyme and Carbon Nanotube Surface Through a Pyrene Derivative

2019 ◽  
Vol 19 (6) ◽  
pp. 3551-3557 ◽  
Author(s):  
Hiroaki Sakamoto ◽  
Ayako Koto ◽  
Ei-Ichiro Takamura ◽  
Hitoshi Asakawa ◽  
Takeshi Fukuma ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Carbon Nanotube ◽  
Glucose Dehydrogenase ◽  
Molecular Layer ◽  
Biofuel Cells ◽  
Biofuel Cell ◽  
Similar Process ◽  
Enzyme Complex ◽  
Catalytic Current ◽  
Oriented Immobilization

For increasing the output of biofuel cells, increasing the cooperation between enzyme reaction and electron transfer on the electrode surface is essential. Highly oriented immobilization of enzymes onto a carbon nanotube (CNT) with a large specific surface area and excellent conductivity would increase the potential for their application as biosensors and biofuel cells, by utilizing the electron transfer between the electrode-molecular layer. In this study, we prepared a CNT-enzyme complex with highly oriented immobilization of enzyme onto the CNT surface. The complex showed excellent electrical characteristics, and could be used to develop biodevices that enable efficient electron transfer. Multi-walled carbon nanotubes (MWCNT) were dispersed by pyrene butyric acid N-hydroxysuccinimide ester, and then N-(5-amino-1-carboxypentyl) iminodiacetic acid (AB-NTA) and NiCl2 were added to modify the NTA-Ni2+ complex on the CNT surface. Pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase (GDH) was immobilized on the CNT surface through a genetically introduced His-tag. Formation of the MWCNT-enzyme complex was confirmed by monitoring the catalytic current electrochemically to indicate the enzymatic activity. PQQ-GDH was also immobilized onto a highly ordered pyrolytic graphite surface using a similar process, and the enzyme monolayer was visualized by atomic force microscopy to confirm its structural properties. A biofuel cell was constructed using the prepared CNT-enzyme complex and output evaluation was carried out. As a result, an output of 32 μW/cm2 could be obtained without mediators.

Direct electron transfer-type dual gas diffusion H2/O2biofuel cells

2016 ◽  
Vol 4 (22) ◽  
pp. 8742-8749 ◽  
Author(s):  
Keisei So ◽  
Yuki Kitazumi ◽  
Osamu Shirai ◽  
Koji Nishikawa ◽  
Yoshiki Higuchi ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Direct Electron Transfer ◽  
Environmentally Friendly ◽  
Gas Diffusion ◽  
Biofuel Cells ◽  
Multicopper Oxidases ◽  
Power Devices

H2/O2biofuel cells utilizing hydrogenases and multicopper oxidases as bioelectrocatalysts are clean, sustainable, and environmentally friendly power devices.

Direct electron transfer-based bioanodes for ethanol biofuel cells using PQQ-dependent alcohol and aldehyde dehydrogenases

2013 ◽  
Vol 87 ◽  
pp. 323-329 ◽  
Author(s):  
Sidney Aquino Neto ◽  
Emily L. Suda ◽  
Shuai Xu ◽  
Matthew T. Meredith ◽  
Adalgisa R. De Andrade ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Direct Electron Transfer ◽  
Biofuel Cells ◽  
Aldehyde Dehydrogenases

Gold-Coated M13 Bacteriophage as a Template for Glucose Oxidase Biofuel Cells with Direct Electron Transfer

ACS Nano ◽  
2015 ◽  
Vol 10 (1) ◽  
pp. 324-332 ◽  
Author(s):  
Rita A. Blaik ◽  
Esther Lan ◽  
Yu Huang ◽  
Bruce Dunn
Keyword(s):  
Electron Transfer ◽  
Glucose Oxidase ◽  
Direct Electron Transfer ◽  
Biofuel Cells ◽  
M13 Bacteriophage
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