Development of a DNA Microarray Chip for the Identification of Sludge Bacteria Using an Unsequenced Random Genomic DNA Hybridization Method

2004 ◽  
Vol 38 (24) ◽  
pp. 6767-6774 ◽  
Author(s):  
Byoung Chan Kim ◽  
Ji Hyun Park ◽  
Man Bock Gu
Keyword(s):  
Dna Microarray ◽  
Dna Hybridization ◽  
Genomic Dna ◽  
Hybridization Method ◽  
Microarray Chip

scholarly journals Identification of Yersinia enterocolitica using a random genomic DNA microarray chip

2010 ◽  
Vol 51 (6) ◽  
pp. 665-670 ◽  
Author(s):  
J. Bang ◽  
L.R. Beuchat ◽  
M.B. Gu ◽  
H.-I. Chang ◽  
J.-H. Ryu
Keyword(s):  
Yersinia Enterocolitica ◽  
Dna Microarray ◽  
Genomic Dna ◽  
Microarray Chip

Detection of Alicyclobacillus Species in Fruit Juice Using a Random Genomic DNA Microarray Chip

2011 ◽  
Vol 74 (6) ◽  
pp. 933-938 ◽  
Author(s):  
JUN HYEONG JANG ◽  
SUN-JOONG KIM ◽  
BO HYUN YOON ◽  
JEE-HOON RYU ◽  
MAN BOCK GU ◽  
...  
Keyword(s):  
Dna Microarray ◽  
Orange Juice ◽  
Genomic Dna ◽  
Fruit Juice ◽  
Molar Ratio ◽  
Food Spoilage ◽  
Good Tool ◽  
Microarray Chip ◽  
Spoilage Bacteria ◽  
Bacillus Spp

This study describes a method using a DNA microarray chip to rapidly and simultaneously detect Alicyclobacillus species in orange juice based on the hybridization of genomic DNA with random probes. Three food spoilage bacteria were used in this study: Alicyclobacillus acidocaldarius, Alicyclobacillus acidoterrestris, and Alicyclobacillus cycloheptanicus. The three Alicyclobacillus species were adjusted to 2 × 103 CFU/ml and inoculated into pasteurized 100% pure orange juice. Cy5-dCTP labeling was used for reference signals, and Cy3-dCTP was labeled for target genomic DNA. The molar ratio of 1:1 of Cy3-dCTP and Cy5-dCTP was used. DNA microarray chips were fabricated using randomly fragmented DNA of Alicyclobacillus spp. and were hybridized with genomic DNA extracted from Bacillus spp. Genomic DNA extracted from Alicyclobacillus spp. showed a significantly higher hybridization rate compared with DNA of Bacillus spp., thereby distinguishing Alicyclobacillus spp. from Bacillus spp. The results showed that the microarray DNA chip containing randomly fragmented genomic DNA was specific and clearly identified specific food spoilage bacteria. This microarray system is a good tool for rapid and specific detection of thermophilic spoilage bacteria, mainly Alicyclobacillus spp., and is useful and applicable to the fruit juice industry.

DNA: DNA hybridization method for the diagnosis of hepatitis b infection

1984 ◽  
Vol 8 (3) ◽  
pp. 199-206 ◽  
Author(s):  
S.V. Feinman ◽  
B. Berris ◽  
A. Guha ◽  
R. Sooknanan ◽  
D.W. Bradley ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Dna Hybridization ◽  
Hepatitis B Infection ◽  
Hybridization Method

Genomic variability within laboratory and wild subspecies of Heligmosomoides polygyrus

1994 ◽  
Vol 68 (2) ◽  
pp. 93-96 ◽  
Author(s):  
M.A. Abu-Madi ◽  
A.P. Reid ◽  
J.W. Lewis ◽  
W.M. Hominick
Keyword(s):  
Restriction Endonuclease ◽  
Ribosomal Dna ◽  
Dna Hybridization ◽  
Genomic Dna ◽  
Dna Probes ◽  
Heligmosomoides Polygyrus ◽  
Banding Patterns ◽  
Genomic Variability

AbstractGenomic DNA extracted from laboratory and wild subspecies of the trichostrongyle nematode Heligmosomoides polygyrus were compared using RFLP and DNA/DNA hybridization techniques. Eight restriction endonuclease digests of the genomic DNA of the two subspecies were hybridized with heterologous ribosomal DNA probes and the total radio-isotope labelled DNA of the laboratory subspecies. DNA hybridization of the two subspecies of H. polygyrus yielded different banding patterns when probed with the rDNA clones in Pvu II digests and when total genomic DNA was used as the probe in Hind III and Pvu II digests. The remaining hybridization profiles of both subspecies were identical.

scholarly journals The use of fluorescein for labeling genomic probes in the checkerboard DNA–DNA hybridization method

2008 ◽  
Vol 163 (4) ◽  
pp. 403-407 ◽  
Author(s):  
Cássio do Nascimento ◽  
Rodrigo Edson Santos Barbosa ◽  
João Paulo Mardegan Issa ◽  
Evandro Watanabe ◽  
Izabel Yoko Ito ◽  
...  
Keyword(s):  
Dna Hybridization ◽  
Hybridization Method ◽  
Genomic Probes
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