Multiple and Simultaneous Detection of Specific Bacteria in Enriched Bacterial Communities Using a DNA Microarray Chip with Randomly Generated Genomic DNA Probes

2005 ◽  
Vol 77 (8) ◽  
pp. 2311-2317 ◽  
Author(s):  
Byoung Chan Kim ◽  
Ji Hyun Park ◽  
Man Bock Gu
Keyword(s):  
Dna Microarray ◽  
Bacterial Communities ◽  
Genomic Dna ◽  
Simultaneous Detection ◽  
Dna Probes ◽  
Microarray Chip

scholarly journals Identification of Yersinia enterocolitica using a random genomic DNA microarray chip

2010 ◽  
Vol 51 (6) ◽  
pp. 665-670 ◽  
Author(s):  
J. Bang ◽  
L.R. Beuchat ◽  
M.B. Gu ◽  
H.-I. Chang ◽  
J.-H. Ryu
Keyword(s):  
Yersinia Enterocolitica ◽  
Dna Microarray ◽  
Genomic Dna ◽  
Microarray Chip

Detection of Alicyclobacillus Species in Fruit Juice Using a Random Genomic DNA Microarray Chip

2011 ◽  
Vol 74 (6) ◽  
pp. 933-938 ◽  
Author(s):  
JUN HYEONG JANG ◽  
SUN-JOONG KIM ◽  
BO HYUN YOON ◽  
JEE-HOON RYU ◽  
MAN BOCK GU ◽  
...  
Keyword(s):  
Dna Microarray ◽  
Orange Juice ◽  
Genomic Dna ◽  
Fruit Juice ◽  
Molar Ratio ◽  
Food Spoilage ◽  
Good Tool ◽  
Microarray Chip ◽  
Spoilage Bacteria ◽  
Bacillus Spp

This study describes a method using a DNA microarray chip to rapidly and simultaneously detect Alicyclobacillus species in orange juice based on the hybridization of genomic DNA with random probes. Three food spoilage bacteria were used in this study: Alicyclobacillus acidocaldarius, Alicyclobacillus acidoterrestris, and Alicyclobacillus cycloheptanicus. The three Alicyclobacillus species were adjusted to 2 × 103 CFU/ml and inoculated into pasteurized 100% pure orange juice. Cy5-dCTP labeling was used for reference signals, and Cy3-dCTP was labeled for target genomic DNA. The molar ratio of 1:1 of Cy3-dCTP and Cy5-dCTP was used. DNA microarray chips were fabricated using randomly fragmented DNA of Alicyclobacillus spp. and were hybridized with genomic DNA extracted from Bacillus spp. Genomic DNA extracted from Alicyclobacillus spp. showed a significantly higher hybridization rate compared with DNA of Bacillus spp., thereby distinguishing Alicyclobacillus spp. from Bacillus spp. The results showed that the microarray DNA chip containing randomly fragmented genomic DNA was specific and clearly identified specific food spoilage bacteria. This microarray system is a good tool for rapid and specific detection of thermophilic spoilage bacteria, mainly Alicyclobacillus spp., and is useful and applicable to the fruit juice industry.

Genomic variability within laboratory and wild subspecies of Heligmosomoides polygyrus

1994 ◽  
Vol 68 (2) ◽  
pp. 93-96 ◽  
Author(s):  
M.A. Abu-Madi ◽  
A.P. Reid ◽  
J.W. Lewis ◽  
W.M. Hominick
Keyword(s):  
Restriction Endonuclease ◽  
Ribosomal Dna ◽  
Dna Hybridization ◽  
Genomic Dna ◽  
Dna Probes ◽  
Heligmosomoides Polygyrus ◽  
Banding Patterns ◽  
Genomic Variability

AbstractGenomic DNA extracted from laboratory and wild subspecies of the trichostrongyle nematode Heligmosomoides polygyrus were compared using RFLP and DNA/DNA hybridization techniques. Eight restriction endonuclease digests of the genomic DNA of the two subspecies were hybridized with heterologous ribosomal DNA probes and the total radio-isotope labelled DNA of the laboratory subspecies. DNA hybridization of the two subspecies of H. polygyrus yielded different banding patterns when probed with the rDNA clones in Pvu II digests and when total genomic DNA was used as the probe in Hind III and Pvu II digests. The remaining hybridization profiles of both subspecies were identical.

Simultaneous detection ofAureliaandChrysaorascyphozoan jellyfish on a DNA microarray

2009 ◽  
Vol 90 (6) ◽  
pp. 1111-1117 ◽  
Author(s):  
Jang-Seu Ki ◽  
Dae-Sik Hwang ◽  
Jae-Seong Lee
Keyword(s):  
Dna Microarray ◽  
Simultaneous Detection ◽  
Dna Chip ◽  
Coi Gene ◽  
Low Density ◽  
Sequence Comparisons ◽  
Mitochondrial Coi ◽  
Pcr Products ◽  
Jellyfish Species ◽  
Species Specific

To demonstrate the effectiveness of microarrays for the detection of jellyfish, we developed a low density DNA chip based on the mitochondrial COI gene sequences of scyphozoans (jellyfish). We designed species-specific oligonucleotide probes by sequence comparisons between scyphozoans and other cnidarians such as hydrozoans and anthozoans. Each amine-labelled capture probe was arrayed onto a silylated slide. PCR products of the COI gene were hybridized to the DNA microarray that contained COI consensus sequences. We tested the ability of the DNA chip to discriminate between species from the generaAureliaandChrysaorabased on samples of both species from the polyp and ephyra stages. The array produced unique hybridization patterns for each of the two tested jellyfish species. Furthermore, we were able to simultaneously detect individual jellyfish species from mixtures of these two different species in the laboratory and from environmental samples. These results show that the low density DNA chip that we designed can be used as a technical platform for parallel molecular detection of various jellyfish species.

scholarly journals Development of a multiplex PCR for simultaneous detection of Pasteurella multocida, Mannheimia haemolytica and Trueperella pyogenes

2017 ◽  
Vol 65 (3) ◽  
pp. 327-339 ◽  
Author(s):  
Wenlong Zhang ◽  
Xiaodan Liu ◽  
Mengcheng Liu ◽  
Bo Ma ◽  
Li Xu ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Genomic Dna ◽  
Pasteurella Multocida ◽  
Bacterial Pathogens ◽  
Simultaneous Detection ◽  
Laboratory Diagnosis ◽  
Mannheimia Haemolytica ◽  
Bacterial Strains ◽  
Trueperella Pyogenes ◽  
Enrichment Technology

Pasteurella multocida, Mannheimia haemolytica and Trueperella pyogenes are three bacterial pathogens closely associated with the bovine respiratory disease complex (BRDC). In the current study, a multiplex PCR for the simultaneous detection of these three bacteria in cultures was established. After serial optimisation, the detection limit of the method for the genomic DNA of the three bacteria was 40 pg/μl. The method could detect the genomic DNA of these three bacteria but not the genomic DNA of seven other bacterial strains. Together with the bacterial enrichment technology, the multiplex PCR could be used for detecting the three bacteria in animal tissues. This method might be valuable for speeding up laboratory diagnosis and directing the treatment of BRDC to these three bacterial pathogens.

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