Time-Resolved Characterization of Diesel Particulate Emissions. 2. Instruments for Elemental and Organic Carbon Measurements

2001 ◽  
Vol 35 (10) ◽  
pp. 1935-1942 ◽  
Author(s):  
H. Moosmüller ◽  
W. P. Arnott ◽  
C. F. Rogers ◽  
J. L. Bowen ◽  
J. A. Gillies ◽  
...  
Keyword(s):  
Organic Carbon ◽  
Particulate Emissions ◽  
Time Resolved ◽  
Diesel Particulate

Time Resolved Characterization of Diesel Particulate Emissions. 1. Instruments for Particle Mass Measurements

2001 ◽  
Vol 35 (4) ◽  
pp. 781-787 ◽  
Author(s):  
H. Moosmüller ◽  
W. P. Arnott ◽  
C. F. Rogers ◽  
J. L. Bowen ◽  
J. A. Gillies ◽  
...  
Keyword(s):  
Particle Mass ◽  
Particulate Emissions ◽  
Mass Measurements ◽  
Time Resolved ◽  
Diesel Particulate

Characterization of engine oil additive packages on diesel particulate emissions

2020 ◽  
Vol 34 (2) ◽  
pp. 931-939 ◽  
Author(s):  
Kangjin Kim ◽  
Woosung Si ◽  
Dongyoung Jin ◽  
Jeong-Hwan Kim ◽  
Jaeho Cho ◽  
...  
Keyword(s):  
Engine Oil ◽  
Particulate Emissions ◽  
Diesel Particulate

The Morphology and Crystallography of Diesel Particulate Emissions

1981 ◽  
Vol 39 ◽  
pp. 148-149
Author(s):  
R. Stevenson
Keyword(s):  
Diffraction Pattern ◽  
Carbon Films ◽  
Particulate Emissions ◽  
Diesel Particulate ◽  
Amorphous Carbon Films ◽  
Turbostratic Carbon ◽  
Diesel Particles ◽  
Tem Mode ◽  
Exhaust Stream ◽  
Diffraction Patterns

A study has been made of the morphology and crystallography of particulate emissions from indirect injection diesel engines. This particulate matter consists substantially of carbon (although hydrocarbons can be extracted with solvents). Samples were collected in a diluted exhaust stream on amorphous carbon films and examined in a JEM-200C electron microscope operated in the TEM mode with an accelerating voltage of 200 KV.The morphology of the diesel particles, as shown in Fig. 1, markedly resembles carbon blacks and consists of an agglomeration of quasispherical subunits arranged in chains or clusters. Only limited changes in morphology were observed as the number of subunits in the particle increased (although larger particles tended to be more cluster-like than the extended chain shown in Fig. 1). However, a dramatic effect of the number of subunits was observed on the character of the diffraction pattern. Smaller particles yielded a diffraction pattern consisting of very diffuse rings typical of turbostratic carbon; the diffraction patterns from the larger particles, however, although qualitatively similar, exhibited much sharper and less diffuse ring patterns.

scholarly journals Characterization of a Novel FRET Probe in a Crowded Environment using Time-Resolved Anisotropy

2017 ◽  
Vol 112 (3) ◽  
pp. 346a
Author(s):  
Hannah Leopold ◽  
Megan Currie ◽  
Jacob Schwarz ◽  
Arnold J. Boersma ◽  
Erin D. Sheets ◽  
...  
Keyword(s):  
Time Resolved ◽  
Crowded Environment

scholarly journals KD determination from time-resolved experiments on live cells with LigandTracer and reconciliation with end-point flow cytometry measurements

2021 ◽  
Author(s):  
Diana Spiegelberg ◽  
Jonas Stenberg ◽  
Pascale Richalet ◽  
Marc Vanhove
Keyword(s):  
Flow Cytometry ◽  
Live Cells ◽  
Time Resolved ◽  
Surface Receptors ◽  
Full Characterization ◽  
End Point ◽  
Titration Curves ◽  
Kinetics Of

AbstractDesign of next-generation therapeutics comes with new challenges and emulates technology and methods to meet them. Characterizing the binding of either natural ligands or therapeutic proteins to cell-surface receptors, for which relevant recombinant versions may not exist, represents one of these challenges. Here we report the characterization of the interaction of five different antibody therapeutics (Trastuzumab, Rituximab, Panitumumab, Pertuzumab, and Cetuximab) with their cognate target receptors using LigandTracer. The method offers the advantage of being performed on live cells, alleviating the need for a recombinant source of the receptor. Furthermore, time-resolved measurements, in addition to allowing the determination of the affinity of the studied drug to its target, give access to the binding kinetics thereby providing a full characterization of the system. In this study, we also compared time-resolved LigandTracer data with end-point KD determination from flow cytometry experiments and hypothesize that discrepancies between these two approaches, when they exist, generally come from flow cytometry titration curves being acquired prior to full equilibration of the system. Our data, however, show that knowledge of the kinetics of the interaction allows to reconcile the data obtained by flow cytometry and LigandTracer and demonstrate the complementarity of these two methods.

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