Characterization of the H4IIE rat hepatoma cell bioassay as a tool for assessing toxic potency of planar halogenated hydrocarbons in environmental samples

1991 ◽  
Vol 25 (1) ◽  
pp. 87-92 ◽  
Author(s):  
Donald E. Tillitt ◽  
John P. Giesy ◽  
Gerald T. Ankley
Keyword(s):  
Environmental Samples ◽  
Hepatoma Cell ◽  
Halogenated Hydrocarbons ◽  
Rat Hepatoma

Partial characterization of rat hepatoma cell-surface glycopeptides possessing concanavalin a receptor activity

1976 ◽  
Vol 72 (1) ◽  
pp. 81-88 ◽  
Author(s):  
John M. Robinson ◽  
David F. Smith ◽  
Edward M. Davis ◽  
Ellen B. Gilliam ◽  
Sylvia C. Capetillo ◽  
...  
Keyword(s):  
Cell Surface ◽  
Concanavalin A ◽  
Hepatoma Cell ◽  
Receptor Activity ◽  
Partial Characterization ◽  
Rat Hepatoma

scholarly journals Characterization of aflatoxin B1-induced rat hepatoma cell line

1992 ◽  
Vol 1992 (35) ◽  
pp. 19-28
Author(s):  
Fumio TASHIRO ◽  
Eriko TANAKA ◽  
Naomitsu HIRANO ◽  
Kiyomitsu NEMOTO ◽  
Tohru YAMAKUNI ◽  
...  
Keyword(s):  
Cell Line ◽  
Aflatoxin B1 ◽  
Hepatoma Cell ◽  
Hepatoma Cell Line ◽  
Rat Hepatoma Cell Line ◽  
Rat Hepatoma

scholarly journals Bioanalytical screening of low levels of dioxins and dioxin-like PCBs in pig meat (pork) for checking compliance with EU maximum and action levels using highly sensitive “third generation” recombinant H4L7.5c2 rat hepatoma cells

2021 ◽  
Vol 33 (1) ◽  
Author(s):  
Johannes Haedrich ◽  
Claudia Stumpf ◽  
Michael S. Denison
Keyword(s):  
European Union ◽  
Hepatoma Cell ◽  
Animal Origin ◽  
The European Union ◽  
Highly Sensitive ◽  
Commission Regulation ◽  
Pig Meat ◽  
Low Levels ◽  
Action Levels ◽  
Rat Hepatoma

Abstract Background Low maximum and action levels set by the European Union for polychlorinated dibenzo-p-dioxins and dibenzofurans (PCDD/Fs) and dioxin-like polychlorinated biphenyls (DL-PCBs) in pig meat (pork) have led to a demand for reliable and cost-effective bioanalytical screening methods implemented upstream of gas chromatography/high-resolution mass spectrometry confirmatory technology, that can detect low levels of contamination in EU-regulated foods with quick turn-around times. Results Based on the Chemically Activated LUciferase gene eXpression (CALUX) bioassay, extraction and clean-up steps were optimized for recovery and reproducibility within working ranges significantly lower than in current bioassays. A highly sensitive “3rd generation” recombinant rat hepatoma cell line (H4L7.5c2) containing 20 dioxin responsive elements was exposed to pork sample extracts, and their PCDD/Fs and DL-PCBs levels were evaluated by measuring luciferase activity. The method was validated according to the provisions of Commission Regulation (EU) 2017/644 of 5 April 2017 with spiking experiments performed selectively for PCDD/Fs and DL-PCBs and individual calibration for PCDD/Fs, DL-PCBs and the calculated sum of PCDD/Fs and DL-PCBs. The resulting performance parameters met all legal specifications as confirmed by re-calibration using authentic samples. Cut-off concentrations for assessing compliance with low maximum levels and action levels set for PCDD/Fs and DL-PCBs within a range of 0.50–1.25 pg WHO-TEQ/g fat were derived, ensuring low rates of false-compliant results (ß-error < 1%) and keeping the rate of false-noncompliant results well under control (α-error < 12%). Conclusions We present a fast and efficient bioanalytical routine method validated according to the European Union’s legal requirements on the basis of authentic samples, allowing the analyst to reliably identify pork samples and any other EU-regulated foods of animal origin suspected to be noncompliant with a high level of performance and turn-around times of 52 h. This was facilitated in particular by a quick and efficient extraction step followed by selective clean-up, use of a highly sensitive “3rd generation” H4L7.5c2 recombinant rat hepatoma cell CALUX bioassay, and optimized assay performance with improved calibrator precision and reduced lack-of-fit errors. New restrictions are proposed for the calibrator bias and the unspecific background contribution to reportable results. The procedure can utilize comparably small sample amounts and allows an annual throughput of 840–1000 samples per lab technician. The described bioanalytical method contributes to the European Commission's objective of generating accurate and reproducible analytical results according to Commission Regulation (EU) 2017/644 across the European Union.

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