In-Situ Biodegradation of Toluene in a Contaminated Stream. 2. Laboratory Studies

1995 ◽  
Vol 29 (1) ◽  
pp. 117-125 ◽  
Author(s):  
Brian A. Cohen ◽  
Lee R. Krumholz ◽  
Heekyung. Kim ◽  
Harold F. Hemond
Keyword(s):  
Laboratory Studies ◽  
In Situ Biodegradation

Metagenomics Study To Compare The Taxonomy And Metabolism of a Lignocellulolytic Microbial Consortium Cultured in Different Carbon Conditions

2021 ◽  
Author(s):  
Qinggeer BORJIGIN ◽  
Bizhou ZHANG ◽  
Xiaofang Yu ◽  
Julin Gao ◽  
Xin ZHANG ◽  
...  
Keyword(s):  
Microbial Consortium ◽  
Agricultural Waste ◽  
Taxonomic Diversity ◽  
Taxonomic Composition ◽  
Microbial Consortia ◽  
Rich Diversity ◽  
Cazy Database ◽  
Taxonomic Affiliation ◽  
In Situ Biodegradation

Abstract A lignocellulolytic microbial consortium holds promise for the in situ biodegradation of crop straw and the comprehensive and effective utilization of agricultural waste. In this study, we applied metagenomics technology to comprehensively explore the metabolic functional potential and taxonomic diversity of the microbial consortia CS (cultured on corn stover) and FP (cultured on filter paper).Analyses of the metagenomics taxonomic affiliation data showed considerable differences in the taxonomic composition and functional profile of the microbial consortia CS and FP. The microbial consortia CS primarily contained members from the genera Pseudomonas, Stenotrophomonas, Achromobacter, Dysgonomonas, Flavobacterium and Sphingobacterium, as well as Cellvibrio, Azospirillum, Pseudomonas, Dysgonomonas and Cellulomonas in FP. The COG and KEGG annotation analyses revealed considerable levels of diversity. Further analysis determined that the CS consortium had an increase in the acid and ester metabolism pathways, while carbohydrate metabolism was enriched in the FP consortium. Furthermore, a comparison against the CAZy database showed that the microbial consortia CS and FP contain a rich diversity of lignocellulose degrading families, in which GH5, GH6, GH9, GH10, GH11, GH26, GH42, and GH43 were enriched in the FP consortium, and GH44, GH28, GH2, and GH29 increased in the CS consortium. The degradative mechanism of lignocellulose metabolism by the two microbial consortia is similar, but the annotation of quantity of genes indicated that they are diverse and vary greatly. The lignocellulolytic microbial consortia cultured under different carbon conditions (CS and FP) differed substantially in their composition of the microbial community at the genus level. The changes in functional diversity were accompanied with variation in the composition of microorganisms, many of which are related to the degradation of lignocellulolytic materials. The genera Pseudomonas, Dysgonomonas and Sphingobacterium in CS and the genera Cellvibrio and Pseudomonas in FP exhibited a much wider distribution of lignocellulose degradative ability.

scholarly journals In situ biodegradation, photooxidation and dissolution of petroleum compounds in Arctic seawater and sea ice

2019 ◽  
Vol 148 ◽  
pp. 459-468 ◽  
Author(s):  
Leendert Vergeynst ◽  
Jan H. Christensen ◽  
Kasper Urup Kjeldsen ◽  
Lorenz Meire ◽  
Wieter Boone ◽  
...  
Keyword(s):  
Sea Ice ◽  
In Situ Biodegradation

scholarly journals How Early Diagenesis Reveals in Situ Biodegradation of Herbicides in Sediment

10.5772/13298 ◽  
2011 ◽  
Author(s):  
Devault Damien ◽  
Delmotte Sbastien ◽  
Macarie Herv ◽  
Dolfing Jan ◽  
Anschutz Pierre
Keyword(s):  
Early Diagenesis ◽  
In Situ Biodegradation

Studies on radionuclide transport behaviour–The next generation of in-situ experiments at the Grimsel Test Site

2009 ◽  
Vol 1193 ◽  
Author(s):  
Andrew James Martin ◽  
Ingo Blechschmidt
Keyword(s):  
Shear Zone ◽  
Diffusion Processes ◽  
Test Site ◽  
Core Material ◽  
Laboratory Studies ◽  
Grimsel Test Site ◽  
And Migration ◽  
Colloid Formation

AbstractTwo recent ongoing major projects at the Grimsel Test Site (GTS) (www.grimsel.com) that were initiated to simulate the long-term behaviour of radionuclides in the repository near-field and the surrounding host rock are presented: the Colloid Formation and Migration (CFM) project, which focuses on colloid generation and migration from a bentonite source doped with radionuclides and the Long-Term Diffusion (LTD) project, which aims at in-situ verification and understanding of the processes that control the long-term diffusion of repository-relevant radionuclides. So far, the CFM project has principally involved: development and implementation of a state-of-the-art sealing concept to control hydraulic gradients in a shear zone to imitate repository-relevant conditions; extensive laboratory studies to examine bentonite erosion and colloid formation in a shear zone; and, development of models to estimate colloid formation and migration. The next stage will be to assess the behavior of bentonite colloids generated from a radionuclide spiked bentonite source-term emplaced into the controlled flow field of the shear zone. This will be coupled with further extensive laboratory studies in order to refine and evaluate the colloid models currently used in performance assessments. The LTD project consists of: a monopole diffusion experiment where weakly sorbing and non-sorbing radionuclides (3H, 22Na, 131I, 134Cs) have been circulating and diffusing into undisturbed rock matrix since June 2007; experiments to characterise pore space geometry, including determination of in-situ porosity with 14C doped MMA resin for comparison with laboratory derived data; a study of natural tracers to elucidate evidence of long-term diffusion processes; and, an investigation of the in-situ matrix diffusion paths in core material from earlier GTS experiments. Future experiments will focus on diffusion processes starting from a water-conducting feature under realistic boundary conditions.

Numerical analysis of in-situ biodegradation model in porous media

2018 ◽  
Vol 344 ◽  
pp. 190-228
Author(s):  
Abdellatif Agouzal ◽  
Karam Allali ◽  
Siham Binna
Keyword(s):  
Porous Media ◽  
Numerical Analysis ◽  
In Situ Biodegradation
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