A Scraped Surface Bioreactor for Enzymatic Saccharification of Pretreated Corn Stover Slurries

2009 ◽  
Vol 23 (1) ◽  
pp. 492-497 ◽  
Author(s):  
Rajesh K. Dasari ◽  
Kyle Dunaway ◽  
R. Eric Berson
Keyword(s):  
Corn Stover ◽  
Enzymatic Saccharification ◽  
Pretreated Corn Stover

Countercurrent enzymatic saccharification of pretreated corn stover. Part 2: Lime + shock pretreated corn stover and commercial approach

2017 ◽  
Vol 98 ◽  
pp. 124-134 ◽  
Author(s):  
Chao Liang ◽  
Sagar Lonkar ◽  
Pratik Darvekar ◽  
Austin Bond ◽  
Agustin N. Zentay ◽  
...  
Keyword(s):  
Corn Stover ◽  
Enzymatic Saccharification ◽  
Pretreated Corn Stover

Fractal kinetic analysis of the enzymatic saccharification of CO2 laser pretreated corn stover

2013 ◽  
Vol 98 (1) ◽  
pp. 618-623 ◽  
Author(s):  
Shuang-Qi Tian ◽  
Sen Ma ◽  
Xin-Wei Wang ◽  
Zheng-Nan Zhang
Keyword(s):  
Kinetic Analysis ◽  
Corn Stover ◽  
Co2 Laser ◽  
Enzymatic Saccharification ◽  
Pretreated Corn Stover

Countercurrent enzymatic saccharification of pretreated corn stover part 2: Lime + shock pretreated corn stover and commercial approach

2017 ◽  
Vol 97 ◽  
pp. 43-52 ◽  
Author(s):  
Chao Liang ◽  
Sagar Lonkar ◽  
Pratik Darvekar ◽  
Austin Bond ◽  
Agustin N. Zentay ◽  
...  
Keyword(s):  
Corn Stover ◽  
Enzymatic Saccharification ◽  
Pretreated Corn Stover

Enzymatic saccharification of pretreated corn stover in a fed-batch membrane bioreactor

2010 ◽  
Vol 4 (2) ◽  
pp. 134-140 ◽  
Author(s):  
Mingjia Zhang ◽  
Rongxin Su ◽  
Qiang Li ◽  
Wei Qi ◽  
Zhimin He
Keyword(s):  
Corn Stover ◽  
Membrane Bioreactor ◽  
Enzymatic Saccharification ◽  
Fed Batch ◽  
Pretreated Corn Stover

scholarly journals Effects of Additional Xylanase on Saccharification and Ethanol Fermentation of Ammonia-Pretreated Corn Stover and Rice Straw

Energies ◽  
2020 ◽  
Vol 13 (17) ◽  
pp. 4574 ◽  
Author(s):  
Seung Hyeon Park ◽  
Thi Thu Huong Pham ◽  
Tae Hyun Kim
Keyword(s):  
Ethanol Production ◽  
Corn Stover ◽  
Rice Straw ◽  
Enzymatic Saccharification ◽  
Inoculum Size ◽  
Lignocellulosic Material ◽  
Pretreated Corn Stover ◽  
Ethanol Yields ◽  
Saccharification Yield ◽  
Ammonia Method

Synergistic effect of cellulase and hemicellulase (xylanase) was evaluated because lignocellulosic material is a heterogeneous complex of cellulose and hemicellulose. Various effects of HTec2 addition on enzymatic saccharification and fermentation were evaluated using two different substrates such as corn stover and rice straw. Corn stover and rice straw were pretreated by the LMAA (low-moisture anhydrous ammonia) method at the preselected same conditions (90 °C, 120 h, moisture content = 50%, NH3 loading = 0.1 g NH3/g). It was observed that the enzymatic saccharification yield of pretreated corn stover (76.4% for glucan digestibility) was higher than that of pretreated rice straw (70.9% for glucan) using CTec2 cellulase without HTec2 addition. Glucan digestibility of pretreated corn stover was significantly increased from 76.4% to 91.1% when the HTec2/CTec2 (v/v) increased from 0 to 10. However, it was interesting that the ethanol production was decreased from 89.9% to 76.3% for SSF and 118.0% to 87.9% for SSCF at higher HTec2/CTec2. As the glucan loading increased from 2.0% to 7.0%, the ethanol yields of both SSF and SSCF were decreased from 96.3% to 88.9% and from 116.6% to 92.4%, respectively. In addition, the smallest inoculum size (optical density of 0.25) resulted in the highest ethanol production (20.5 g/L).

Enzymatic saccharification of acid pretreated corn stover: Empirical and fractal kinetic modelling

2016 ◽  
Vol 220 ◽  
pp. 110-116 ◽  
Author(s):  
Mateusz Wojtusik ◽  
Mauricio Zurita ◽  
Juan C. Villar ◽  
Miguel Ladero ◽  
Felix Garcia-Ochoa
Keyword(s):  
Corn Stover ◽  
Kinetic Modelling ◽  
Enzymatic Saccharification ◽  
Pretreated Corn Stover

scholarly journals Pretreatment Affects Profits From Xylanase During Enzymatic Saccharification of Corn Stover Through Changing the Interaction Between Lignin and Xylanase Protein

2021 ◽  
Vol 12 ◽  
Author(s):  
Xiaoting Feng ◽  
Yini Yao ◽  
Nuo Xu ◽  
Hexue Jia ◽  
Xuezhi Li ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Corn Stover ◽  
Biomass Conversion ◽  
Enzymatic Saccharification ◽  
Pretreated Corn Stover ◽  
Conversion Rates ◽  
Ammonium Sulfite ◽  
Initial Adsorption ◽  
The Cost ◽  
Hydrolysis Of

Effective pretreatment is vital to improve the biomass conversion efficiency, which often requires the addition of xylanase as an accessory enzyme to enhance enzymatic saccharification of corn stover. In this study, we investigated the effect of two sophisticated pretreatment methods including ammonium sulfite (AS) and steam explosion (SE) on the xylanase profits involved in enzymatic hydrolysis of corn stover. We further explored the interactions between lignin and xylanase Xyn10A protein. Our results showed that the conversion rates of glucan and xylan in corn stover by AS pretreatment were higher by Xyn10A supplementation than that by SE pretreatment. Compared with the lignin from SE pretreated corn stover, the lignin from AS pretreated corn stover had a lower Xyn10A initial adsorption velocity (13.56 vs. 10.89 mg g−1 min−1) and adsorption capacity (49.46 vs. 27.42 mg g−1 of lignin) and weakened binding strength (310.6 vs. 215.9 L g−1). Our study demonstrated the low absolute zeta potential and strong hydrophilicity of the lignin may partly account for relative weak interaction between xylanase protein and lignin from AS pretreated corn stover. In conclusion, our results suggested that AS pretreatment weakened the inhibition of lignin to enzyme, promoted the enzymatic hydrolysis of corn stover, and decreased the cost of enzyme in bioconversion.

scholarly journals Comparison of Corn Stover Pretreatments with Lewis Acid Catalyzed Choline Chloride, Glycerol and Choline Chloride-Glycerol Deep Eutectic Solvent

Polymers ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 1170
Author(s):  
Yuan Zhu ◽  
Benkun Qi ◽  
Xinquan Liang ◽  
Jianquan Luo ◽  
Yinhua Wan
Keyword(s):  
Corn Stover ◽  
Lewis Acid ◽  
Choline Chloride ◽  
Deep Eutectic Solvent ◽  
Enzymatic Saccharification ◽  
Glycerol Solution ◽  
Cellulose Conversion ◽  
Biomass Processing ◽  
Acid Catalyzed ◽  
Highly Correlated

Herein, corn stover (CS) was pretreated by less corrosive lewis acid FeCl3 acidified solutions of neat and aqueous deep eutectic solvent (DES), aqueous ChCl and glycerol at 120 °C for 4 h with single FeCl3 pretreatment as control. It was unexpected that acidified solutions of both ChCl and glycerol were found to be more efficient at removing lignin and xylan, leading to higher enzymatic digestibility of pretreated CS than acidified DES. Comparatively, acidified ChCl solution exhibited better pretreatment performance than acidified glycerol solution. In addition, 20 wt% water in DES dramatically reduced the capability of DES for delignification and xylan removal and subsequent enzymatic cellulose saccharification of pretreated CS. Correlation analysis showed that enzymatic saccharification of pretreated CS was highly correlated to delignification and cellulose crystallinity, but lowly correlated to xylan removal. Recyclability experiments of different acidified pretreatment solutions showed progressive decrease in the pretreatment performance with increasing recycling runs. After four cycles, the smallest decrease in enzymatic cellulose conversion (22.07%) was observed from acidified neat DES pretreatment, while the largest decrease (43.80%) was from acidified ChCl pretreatment. Those findings would provide useful information for biomass processing with ChCl, glycerol and ChCl-glycerol DES.

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