Regeneration of Silica Gel Using High-intensity Ultrasonic under Low Temperatures

2009 ◽  
Vol 23 (1) ◽  
pp. 457-463 ◽  
Author(s):  
Ye Yao ◽  
Shiqing Liu ◽  
Weijiang Zhang
Keyword(s):  
Silica Gel ◽  
High Intensity ◽  
Low Temperatures

scholarly journals Comparison of preservation methods of Atta spp. (Hymenoptera: Formicidae) for RAPD analysis

2000 ◽  
Vol 29 (3) ◽  
pp. 489-496 ◽  
Author(s):  
Alfredo O. R. Carvalho ◽  
Luiz G. E. Vieira
Keyword(s):  
Silica Gel ◽  
Low Temperatures ◽  
Room Temperature ◽  
Rapd Analysis ◽  
Limiting Factors ◽  
Dna Yield ◽  
Remote Locations ◽  
Leaf Cutting ◽  
Storage Methods ◽  
Plot Design

High quality DNA for molecular studies can be easily extracted from fresh specimens. However, live samples are difficult to keep for long periods thus making their preservation a serious problem, specially when they are collected and transported from remote locations. In order to establish an efficient method to preserve Atta spp. (leaf-cutting ants) for RAPD analysis, six different storage methods were examined: 1) -70°C; 2) 95% ethanol at -20°C; 3) 95% ethanol at 4°C; 4) 95% ethanol at room temperature; 5) silica gel at room temperature; and 6) buffer (0.25 M EDTA, 2.5% SDS, 0.5 M Tris-HCl, pH 9.2) at room temperature. DNA was extracted (Cheung et al., 1993 - modified) and examined after 90, 210 and 360 days of storage. Freshly killed specimens were used as control. DNA yield was measured with a minifluorometer. DNA quality was determined by scanning photographs with a densitometer and the integral of the scan was calculated for DNA of size > 9.4 kb. Data were analyzed using a completely randomized split-plot design with four replicates. All methods were efficient to preserve Atta spp. DNA up to 210 days. At 360 days, DNA was degraded only in 95% ethanol at room temperature, which resulted in RAPD profiles with missing bands. Although preservation at low temperatures is recommended for long periods, methods using silica gel and buffer can be considered satisfactory alternatives when refrigeration and transportation are limiting factors.

Luminescence of CuCl at low temperatures excited by very high intensity light (laser)

1967 ◽  
Vol 24 (1) ◽  
pp. 50-52 ◽  
Author(s):  
J.B. Grun ◽  
R. Lévy ◽  
A. Mysyrowicz ◽  
S. Nikitine
Keyword(s):  
High Intensity ◽  
Low Temperatures ◽  
High Intensity Light ◽  
Very High

The energy-saving characteristic of silica gel regeneration with high-intensity ultrasound

2011 ◽  
Vol 88 (6) ◽  
pp. 2146-2156 ◽  
Author(s):  
Weijiang Zhang ◽  
Ye Yao ◽  
Beixing He ◽  
Rongshun Wang
Keyword(s):  
Energy Saving ◽  
Silica Gel ◽  
High Intensity ◽  
High Intensity Ultrasound

Anomalous attenuation at low temperatures in high-intensity helium beam sources

2005 ◽  
Vol 76 (12) ◽  
pp. 123111 ◽  
Author(s):  
H. Hedgeland ◽  
A. P. Jardine ◽  
W. Allison ◽  
J. Ellis
Keyword(s):  
High Intensity ◽  
Low Temperatures ◽  
Anomalous Attenuation
Sign in / Sign up

Export Citation Format

Share Document