scholarly journals A Modern Apparatus for Performing Flash Chromatography: An Experiment for the Organic Laboratory

2013 ◽  
Vol 90 (3) ◽  
pp. 376-378 ◽  
Author(s):  
Gregory R. Naumiec ◽  
Angela N. Del Padre ◽  
Matthew M. Hooper ◽  
Alison St. Germaine ◽  
Brenton DeBoef
Keyword(s):  
Flash Chromatography ◽  
Organic Laboratory ◽  
Modern Apparatus

Isolation of Curcuminoids by Flash Chromatography and Quantification by UPLC-MS/MS Q-TOF

Planta Medica ◽  
2011 ◽  
Vol 77 (05) ◽  
Author(s):  
N Ahmad ◽  
FJ Ahmad ◽  
S Ahmad ◽  
Z Iqbal ◽  
M Shamim ◽  
...  
Keyword(s):  
Flash Chromatography

scholarly journals Gundelia tournefortii: Fractionation, Chemical Composition and GLUT4 Translocation Enhancement in Muscle Cell Line

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3785
Author(s):  
Sleman Kadan ◽  
Sarit Melamed ◽  
Shoshana Benvalid ◽  
Zipora Tietel ◽  
Yoel Sasson ◽  
...  
Keyword(s):  
Glucose Transporter ◽  
Rapid Development ◽  
Muscle Cells ◽  
Flash Chromatography ◽  
Glucose Disposal ◽  
Gas Chromatography Mass Spectrometry ◽  
Glut4 Translocation ◽  
Toxic Concentrations ◽  
L6 Muscle Cells

Type 2 diabetes (T2D) is a chronic metabolic disease, which could affect the daily life of patients and increase their risk of developing other diseases. Synthetic anti-diabetic drugs usually show severe side effects. In the last few decades, plant-derived drugs have been intensively studied, particularly because of a rapid development of the instruments used in analytical chemistry. We tested the efficacy of Gundelia tournefortii L. (GT) in increasing the translocation of glucose transporter-4 (GLUT4) to the myocyte plasma membrane (PM), as a main strategy to manage T2D. In this study, GT methanol extract was sub-fractionated into 10 samples using flash chromatography. The toxicity of the fractions on L6 muscle cells, stably expressing GLUTmyc, was evaluated using the MTT assay. The efficacy with which GLUT4 was attached to the L6 PM was evaluated at non-toxic concentrations. Fraction 6 was the most effective, as it stimulated GLUT4 translocation in the absence and presence of insulin, 3.5 and 5.2 times (at 250 μg/mL), respectively. Fraction 1 and 3 showed no significant effects on GLUT4 translocation, while other fractions increased GLUT4 translocation up to 2.0 times. Gas chromatography–mass spectrometry of silylated fractions revealed 98 distinct compounds. Among those compounds, 25 were considered anti-diabetic and glucose disposal agents. These findings suggest that GT methanol sub-fractions exert an anti-diabetic effect by modulating GLUT4 translocation in L6 muscle cells, and indicate the potential of GT extracts as novel therapeutic agents for T2D.

Separation of molecular tracers sorbed onto atmospheric particulate matter by flash chromatography

2007 ◽  
Vol 387 (5) ◽  
pp. 1855-1861 ◽  
Author(s):  
Audrey Lottmann ◽  
Emilie Cadé ◽  
Majdi Lahd Geagea ◽  
Olivier Delhomme ◽  
Catherine Grand ◽  
...  
Keyword(s):  
Particulate Matter ◽  
Atmospheric Particulate Matter ◽  
Flash Chromatography ◽  
Atmospheric Particulate ◽  
Molecular Tracers

Cyclic ketals of 9-fluorenone: Advanced organic laboratory exercise in synthesis and stereochemical analysis using 13C NMR

1991 ◽  
Vol 68 (9) ◽  
pp. 796 ◽  
Author(s):  
Paul R. Rablen ◽  
Mark A. Deuber ◽  
Ai Ching Lim ◽  
Robert M. Dickson ◽  
Claude E. Wintner
Keyword(s):  
13C Nmr ◽  
Laboratory Exercise ◽  
Stereochemical Analysis ◽  
Organic Laboratory
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