Constructing a LabVIEW-Controlled High-Performance Liquid Chromatography (HPLC) System: An Undergraduate Instrumental Methods Exercise

2011 ◽  
Vol 88 (3) ◽  
pp. 317-318 ◽  
Author(s):  
Eugene T. Smith ◽  
Marc Hill
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Hplc System ◽  
Instrumental Methods

scholarly journals High-Capacity Screening of Arylalkylamine N-Acetyltransferase Inhibitors using a High-Performance Liquid Chromatography System

2000 ◽  
Vol 5 (5) ◽  
pp. 361-368 ◽  
Author(s):  
Gilles Ferry ◽  
Jean A. Boutin
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Enzyme Inhibitor ◽  
False Negative ◽  
High Capacity ◽  
Screening Methods ◽  
Enzymatic System ◽  
Systematic Screening ◽  
Hplc System

Systematic screening is a natural development of any pharmacological program. Most enzyme inhibitor screens use indirect or "aspecific" methods, such as colorimetric or fluorimetric ones. These screening methods cause quite a few false-positive and false-negative hits. In order to limit these as much as possible, we developed a methodology using a high-performance liquid chromatography (HPLC) system for the medium throughput screening of serotonin N-acetyltransferase inhibitors. The core of this screening system is (1) the dramatic shortening of the analytical time down to 100 s per run by using a high-performance analytical column (Turbo), and (2) the use of absorption as opposed to radioactivity for detection of the product of the reaction (N-acetylserotonin). This system permits the analysis of about 1,000 compounds per day to be performed with a single HPLC system. This enzymatic system was taken as an example, because the methodology can be extended to many other enzymes, particularly transferases, phosphatases, and kinases.

Preparation and Characterization of Microcystins-LR by HPLC and HPLC-MS

2011 ◽  
Vol 236-238 ◽  
pp. 120-124
Author(s):  
Si Quan Liu ◽  
Yong Fang Chen ◽  
Li Guo Wang ◽  
Rui Bao Jia
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Water Solution ◽  
Dianchi Lake ◽  
Reverse Phase ◽  
Hplc System

It is well known that microcystins (MCs) are the most abundant toxins produced by cyanobacteria in freshwater. The separation and characterization of MCs isomers are very important to the research of algal pollution in freshwater. In this paper MCs isomers were extracted by using methanol water solution and separated by reverse phase high performance liquid chromatography (HPLC). The different isomers were characterized by using HPLC-MS method. Different ratio of extract solvent and chromatographic conditions were discussed. Five MCs isomers were successfully extracted from cyanobacteria of Dianchi Lake. Three of which were characterized to be MC-RR, MC-YR and MC-LR, 1.5mg (92.3 purity) of MC-LR was prepared by using a semi-preparation HPLC system.

scholarly journals Partial Validation of High Performance Liquid Chromatography for Analysis of Isoniazid in Rat Plasma

2018 ◽  
Vol 16 (1) ◽  
pp. 67
Author(s):  
Novi Yantih ◽  
Siti Hafilah ◽  
Yahdiana Harahap ◽  
WAHONO SUMARYONO ◽  
Rianto Setiabudy
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Rat Plasma ◽  
Limit Of Quantification ◽  
Hplc System ◽  
Accuracy And Precision ◽  
Percent Recovery ◽  
Nicotinic Acid Derivatives ◽  
Linearity Test

Tuberculosis is an infectious disease caused by Mycobacterium tuberculosis. Nicotinic acid derivatives such as isoniazid have the strongest anti-tuberculosis properties. For pharmacokinetics studying of isoniazid (INH), a method is needed to determine the levels of INH in plasma. Objective: The aim of this research is to partial validate of high performance liquid chromatography (HPLC) for analysis of INH in rat plasma. Method: For the preliminary study, rat plasma was used. The HPLC system used is a stationary phase C18 with length 250mm and temperature of 30°C, mobile phase hexane sulphonate acid 20mM pH 2.47–methanol (65:35). The analytical parameters in partial validated were linearity, lower limit of quantification (LLOQ), precision, accuracy, and recovery. Results: The results of linearity test of INH showed r value of 0.9996. LLOQ of this method was 0.1258μg/mL. The resulting accuracy and precision value met FDA requirements with a percent recovery ranging from 96.57–107.99%. Conclusion: The HPLC system was a valid method for analysis of INH in rat plasma.

QUANTITATIVE ANALYSIS OF BETULINIC ACID IN THE PLANT TETRACERA SCANDENS USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

2011 ◽  
pp. 83-88
Author(s):  
Thi Hoai Nguyen
Keyword(s):  
High Performance Liquid Chromatography ◽  
Biological Activity ◽  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
Betulinic Acid ◽  
High Performance ◽  
Aerial Parts ◽  
Hplc System ◽  
The Future

Objective: Betulinic acid is a compound that has many valuable biological activity, the determination of betulinic acid in Tetracera scandens (Dilleniaceae) makes accommodate the exploitation of medicinal will be used in the future. Materials and method: Tetracera scandens had been collected in Huong Thuy District - Thua Thien Hue Province. The quantification were made by a HPLC system using a C18 column. Results: From species Tetracera scandens collected at Huong Thuy - Thua Thien Hue, by a HPLC system, UV-VIS detector, the result showed that accumulation of the principle compound in the aerial parts of the plant is 1.069%.

scholarly journals MODERN INSTRUMENTAL METHODS FOR QUALITATIVE AND QUANTITATIVE ANALYSIS OF LAPATINIB IN BIOLOGICAL FLUIDS AND DOSAGE FORMS (REVIEW)

2022 ◽  
pp. 7-12
Author(s):  
ZOYA S. SHPRAKH ◽  
YANA A. POSKEDOVA ◽  
GALINA V. RAMENSKAYA
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
High Performance ◽  
Biological Fluids ◽  
Dosage Forms ◽  
Forced Degradation ◽  
Qualitative And Quantitative Analysis ◽  
Qualitative And Quantitative ◽  
Instrumental Methods

Lapatinib is a small molecule, a heterocyclic quinazoline derivative. The drug is used for targeted therapy of patients with breast cancer, in which there is overexpression of the human epidermal growth factor receptors (HER/ErbB). This review is devoted to studying modern instrumental methods of qualitative and quantitative analysis of lapatinib, which can be used both for quality control and standardization (of bulk pharmaceuticals and dosage forms) and pharmacokinetics studies of a drug. Reverse-phase high-performance liquid chromatography (RP-HPLC) is mainly used to identify lapatinib in tablets. Depending on the purpose of the study, various detectors are used (ultraviolet or diode-matrix detector), which makes it possible to determine not only the native compound but also the products of its degradation. Definition of lapatinib in the presence of degraded products is necessary for forced degradation studies to determine drug stability. When a drug is being developed, it is important to define and understand its pharmacokinetics. For such studies, high-performance liquid chromatography (HPLC) coupled with the mass selective detector is often used. It allows determining lapatinib in biological fluids. However, these methods are not applicable for identifying the drug directly in dosage forms and require further development and validation.

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