QUANTITATIVE ANALYSIS OF BETULINIC ACID IN THE PLANT TETRACERA SCANDENS USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

2011 ◽  
pp. 83-88
Author(s):  
Thi Hoai Nguyen
Keyword(s):  
High Performance Liquid Chromatography ◽  
Biological Activity ◽  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
Betulinic Acid ◽  
High Performance ◽  
Aerial Parts ◽  
Hplc System ◽  
The Future

Objective: Betulinic acid is a compound that has many valuable biological activity, the determination of betulinic acid in Tetracera scandens (Dilleniaceae) makes accommodate the exploitation of medicinal will be used in the future. Materials and method: Tetracera scandens had been collected in Huong Thuy District - Thua Thien Hue Province. The quantification were made by a HPLC system using a C18 column. Results: From species Tetracera scandens collected at Huong Thuy - Thua Thien Hue, by a HPLC system, UV-VIS detector, the result showed that accumulation of the principle compound in the aerial parts of the plant is 1.069%.

Salting-out induced liquid–liquid microextraction based on the system of acetonitrile/magnesium sulfate for trace-level quantitative analysis of fluoroquinolones in water, food and biological matrices by high-performance liquid chromatography with a fluorescence detector

2014 ◽  
Vol 6 (17) ◽  
pp. 6973-6980 ◽  
Author(s):  
Dongli Du ◽  
Guozhong Dong ◽  
Yuanyuan Wu ◽  
Jingjing Wang ◽  
Ming Gao ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
High Performance ◽  
Biological Matrices ◽  
Fluorescence Detector ◽  
Trace Level ◽  
Salting Out ◽  
Liquid Microextraction

A convenient, economical SILLME method coupled with HPLC/FLD for sample preparation, extraction and trace-level quantitative determination of fluoroquinolones is reported.

scholarly journals Simultaneous Determination of Phenolic Compounds in Leptadenia pyrotechnica (Forssk.) Decne. by Using High-Performance Liquid Chromatography (HPLC-DAD-UV)

2018 ◽  
Vol 2018 ◽  
pp. 1-4
Author(s):  
Raman Preet ◽  
Raghbir Chand Gupta
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Mobile Phase ◽  
High Performance ◽  
Isocratic Elution ◽  
Methanolic Extracts ◽  
Aerial Parts ◽  
Whole Plant

During the present study, an endeavor has been made to produce a simple, rapid, and simultaneous method for determination of phenolic compounds by using high-performance liquid chromatography in aerial parts of Leptadenia pyrotechnica (Forssk.) Decne. collected from the Indian Thar Desert. The optimized process was used for the quantification of ten phenolic compounds. The chromatographic separation was accomplished on an Atlantis T3 column at 25°C with isocratic elution. A mixture of acetonitrile and water was used as the mobile phase at a flow rate of 0.8 mL/min. The linear regression examination data for the calibration plots displayed a good linear relationship with r2 > 0.999 in the concentration range of 2–20 µL. In the methanolic extracts of the whole plant of L. pyrotechnica, the content of caffeic acid (3.3%) was reported to be the highest concentration.

Determination of p-Phenylenediamine Antiozonant in Polychloroprene Rubber

1984 ◽  
Vol 57 (4) ◽  
pp. 804-812 ◽  
Author(s):  
Noel M. Potter ◽  
Ram K. S. Mehta ◽  
Michael G. Wyzgoski
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
High Performance ◽  
Curing Conditions ◽  
Its Analysis ◽  
Mixing Sequence ◽  
Insight Into ◽  
The Way

Abstract Elastomers susceptible to ozone cracking can be protected by the addition of antiozonants. For polychloroprene (CR) rubber, a mixture of diaryl-p-phenyl-enediamines, Wingstay 100 (WS 100), is one of the most effective antiozonants. Preliminary studies in our laboratory have shown that the effectiveness of WS 100 can be improved even further by the use of an organobentonite clay. To provide more insight into the way in which this antiozonant provides resistance to ozone cracking, a technique for its analysis was required. High performance liquid chromatography (HPLC) is an attractive technique for analyzing WS 100, as well as for a wide variety of additives in polymers. This study describes a newly developed method for the determination of WS 100 in CR. The method is based on acetonitrile extraction of the antiozonant from the rubber, followed by quantitative analysis using HPLC. With this method, subtle differences in concentrations of WS 100 in CR, caused by changes in compounding, mixing sequence, curing conditions, and grease extraction, have been measured.

Quantitative Analysis of Flavonoids in the Flowers and Leaves of Ficaria verna Huds.

2003 ◽  
Vol 58 (9-10) ◽  
pp. 763-765 ◽  
Author(s):  
Michał Tomczyk ◽  
Jan Gudej
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Quantitative Analysis ◽  
Quantitative Determination ◽  
High Performance ◽  
Hplc Analysis ◽  
Flavonoid Content ◽  
Growing Seasons ◽  
Ficaria Verna

AbstractThe quantitative determination of flavonoid compounds in flowers and leaves of Ficaria verna Huds. (Ranunculaceae) was carried out in different growing seasons of the plant, using Christ-Müller’s method (Polish Pharmacopoeia, 1999) and high performance liquid chromatography (HPLC) analysis after acid hydrolysis. The flavonoid content was much higher in flowers than in leaves.

scholarly journals DETERMINATION OF LUTEOLIN FROM EXTRACTIONS OF Helicteres hirsuta BY HPLC

2019 ◽  
Vol 128 (1B) ◽  
pp. 43
Author(s):  
Lê Trung Hiếu ◽  
Lê Lâm Sơn ◽  
Nguyễn Minh Nhung ◽  
Hồ Xuân Anh Vũ ◽  
Trần Thị Văn Thi
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Mobile Phase ◽  
High Performance ◽  
Array Detector ◽  
Plant Parts ◽  
Aerial Parts ◽  
Photodiode Array Detector ◽  
Photodiode Array

<p>High performance liquid chromatography coupled with photodiode array detector (HPLC- DAD) has been reported to quantify isolated compounds. This work was designed, therefore, to develop an HPLC-DAD system to determine luteolin in extractive solutions from <em>Helicteres hirsuta</em>. Luteolin was analyzed on a RP- C18 column, using a mobile phase including: acetonitrile - 0.1% acid phosphoric (v/v), under the following conditions detecting wavelength: 347 nm; flow rate: 0.5 mL/min, and the volume of injecting sample: 10 μL. The HPLC system was carried out at ambient temperature. The method showed linearity for luteolin in the range to 0.02 from 1 mg/mL, and the recovery of luteolin was 94.07 ± 0.64. Contents of  luteolin in methanol extracts from the plant parts of <em>H. hirsuta</em> (including: branch, leaf, fruit and aerial parts) were determined with value of 49.06 ±0.46, 142.89 ±0.53, 56.61±0.62 and 91.15±0.42 µg/g, respectively.</p>

scholarly journals Correlations of polyphenolic substances in Ribes aureum Pursh (Grossulariaceae) fruits

2021 ◽  
Vol 40 ◽  
pp. 02010
Author(s):  
Vladimir Sorokopudov ◽  
Alla Kuklina ◽  
Radmil Nigmatzyanov ◽  
Olga Sorokopudova ◽  
Nadezhda Nazaryuk
Keyword(s):  
High Performance Liquid Chromatography ◽  
Biological Activity ◽  
Liquid Chromatography ◽  
High Performance ◽  
Morphometric Parameters ◽  
Reverse Phase ◽  
Carotene Content ◽  
Biochemical Characteristics ◽  
Cultivar Specificity

The morphometric parameters of fruits (yield, weight) were revealed and the quantitative determination of anthocyanins and carotenoids in the fruits of golden currant (Ribes aureum Pursh) was carried out using the methods of spectrophotometry and reverse-phase high-performance liquid chromatography. The cultivar specificity in the content of anthocyanins and carotene in R. aureum fruits was established. There is a direct correlation between the anthocyanin and carotene content, which allows assessing the biological activity more accurately. No correlation was found between the morphometric and biochemical characteristics of R. aureum fruits.

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