Analysis of Volatile Fragrance and Flavor Compounds by Headspace Solid Phase Microextraction and GC-MS: An Undergraduate Instrumental Analysis Experiment

1999 ◽  
Vol 76 (2) ◽  
pp. 245 ◽  
Author(s):  
Randolph C. Galipo ◽  
Alfredo J. Canhoto ◽  
Michael D. Walla ◽  
Stephen L. Morgan
Keyword(s):  
Solid Phase Microextraction ◽  
Solid Phase ◽  
Instrumental Analysis ◽  
Flavor Compounds ◽  
Headspace Solid Phase Microextraction ◽  
Analysis Experiment

scholarly journals GC-MS Characterization of Volatile Flavor Compounds in Stinky Tofu Brine by Optimization of Headspace Solid-Phase Microextraction Conditions

Molecules ◽  
2018 ◽  
Vol 23 (12) ◽  
pp. 3155 ◽  
Author(s):  
Hui Tang ◽  
Jin-Kui Ma ◽  
Lin Chen ◽  
Li-Wen Jiang ◽  
Jing Xie ◽  
...  
Keyword(s):  
Solid Phase Microextraction ◽  
Solid Phase ◽  
Principal Component ◽  
Isobutyric Acid ◽  
Flavor Compounds ◽  
Gas Chromatography Mass Spectrometry ◽  
Extraction Temperature ◽  
Headspace Solid Phase Microextraction ◽  
Volatile Flavor Compounds ◽  
Volatile Flavor

This study optimized the headspace solid phase microextraction (HS-SPME) conditions for the analysis of the volatile flavor compounds of Chinese south stinky tofu brine by gas chromatography-mass spectrometry (GC-MS). The optimum HS-SPME conditions established were as follows: polar column CD-WAX, white 85 μm polyella extractor, extraction temperature 60 °C, equilibrium time 20 min, extraction time 40 min. Under these conditions, a total of 63 volatile flavor compounds in five stinky tofu brines were identified. The offensive odor of the stinky tofu may be derived from some of the volatile flavor compounds such as phenol, p-cresol, 3-methylindole, indole, acetic acid, propionic acid, isobutyric acid, n-butyric acid and 3-methylbutanoic acid. The volatile flavor substances data was examined by principal component analysis (PCA) to visualize the response patterns in the feature space of principal components (PC). PCA analysis results revealed that the Chengshifu brine (STB1) and Baise jingdian brine (STB4) are similar in PC 1, 2, and 3, and the two brines have a similar flavor. Results also indicate that the Huogongdian brine (STB2) and Wangcheng brine (STB3) can be grouped in the same class as they are similar in PC 3. However, PC 1, 2, and 3 of the Luojia brine (STB5) and other brands of brine are different as is the flavor.

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