The determination of the pH of standard buffer solutions: A laboratory experiment

1985 ◽  
Vol 62 (4) ◽  
pp. 350 ◽  
Author(s):  
K. R. Harris
Keyword(s):  
Laboratory Experiment ◽  
Buffer Solutions ◽  
Standard Buffer

A simple method for the determination of the pH values of turbid soil and other solutions

1928 ◽  
Vol 18 (2) ◽  
pp. 206-208 ◽  
Author(s):  
C. H. Gadd
Keyword(s):  
Pathological Condition ◽  
Ratio Method ◽  
Exact Matching ◽  
Simple Method ◽  
Considerable Difficulty ◽  
Buffer Solutions ◽  
Ph Values ◽  
Standard Buffer ◽  
Soil Solutions

In the course of investigations into the cause of an obscure pathological condition of the tea bush, it became necessary to make pH determinations of certain soils. Electrometric apparatus and a suitable centrifuge for clearing the solutions were not available. Considerable difficulty was experienced in making the determinations with accuracy by indicator methods owing to two causes. First, the turbidity of the soil solutions made comparison with standard buffer solutions somewhat difficult; secondly, the colours developed in the soil and buffer solutions with some indicators were different in hue, as distinct from shade or tint. This rendered exact matching quite impossible. The latter difficulties also arose when the drop ratio method was used instead of standard buffer solutions. The errors resulting from these causes were believed to be unduly large, and it was felt that greater accuracy would be attained if these difficulties could be overcome.

Determination of Nitrate and Nitrite in Water by Capillary Electrophoresis: An Undergraduate Laboratory Experiment

1998 ◽  
Vol 75 (12) ◽  
pp. 1588 ◽  
Author(s):  
David S. Hage ◽  
Anuja Chattopadhyay ◽  
Carrie A. C. Wolfe ◽  
Julie Grundman ◽  
Paul Kelter
Keyword(s):  
Capillary Electrophoresis ◽  
Laboratory Experiment ◽  
Undergraduate Laboratory ◽  
Nitrate And Nitrite

scholarly journals Determination of Citrinin in Barley by Indirect and Direct Enzyme Immunoassay

1996 ◽  
Vol 79 (6) ◽  
pp. 1325-1329 ◽  
Author(s):  
David Abramson ◽  
Ewald Usleber ◽  
Erwin Martlbauer
Keyword(s):  
Enzyme Immunoassay ◽  
Solid Phase ◽  
Polyclonal Antibodies ◽  
Detection Limits ◽  
Keyhole Limpet Hemocyanin ◽  
Buffer Solutions ◽  
Microtiter Plates ◽  
Coefficients Of Variation ◽  
Optimum Extraction

Abstract Polyclonal antibodies against the mycotoxin citrinin were raised in rabbits after immunization with citrinin conjugated to keyhole limpet hemocyanin. The antibodies were used in a competitive indirect enzyme immunoassay (EIA) with citrinin coupled to glucose oxidase as the solid-phase antigen for coating microtiter plates. Detection limits of this indirect EIA for citrinin ranged from 0.4 to 0.8 ng/mL for buffer solutions. Recoveries of citrinin added to ground barley at 100–2000 ng/g ranged from 105 to 112%, with coefficients of variation between 4.5 and 12%. A direct competitive EIA also was established, with citrinin coupled to horseradish peroxidase as the labeled antigen. Detection limits of this direct EIA for citrinin ranged from 2 to 4 ng/mL for buffer solutions. Recoveries of citrinin added to ground barley at 500–2000 ng/g ranged from 108 to 111%, with coefficients of variation between 8.4 and 26.9%. In naturally contaminated barley samples assayed with the indirect EIA, optimum extraction of citrinin was obtained in 30 min, and only one extraction was necessary to recover 72–76% of the analyte.

Sign in / Sign up

Export Citation Format

Share Document