An experiment to examine the phosphate interference in the flame emission analysis of calcium

1985 ◽  
Vol 62 (2) ◽  
pp. 161
Author(s):  
Donald C. Jackman
Keyword(s):  
Emission Analysis ◽  
Flame Emission

scholarly journals Comparison of cellular Na+ assays by flow fluorometry with ANG-2 and flame emission: Pitfalls in using ionophores for calibrating fluorescent probes

2020 ◽  
Author(s):  
Valentina E. Yurinskaya ◽  
Nikolay D. Aksenov ◽  
Alexey V. Moshkov ◽  
Tatyana S. Goryachaya ◽  
Alexey A. Vereninov
Keyword(s):  
Flow Cytometry ◽  
Fluorescent Probes ◽  
Single Cells ◽  
Intracellular Sodium ◽  
Cell Populations ◽  
Emission Analysis ◽  
Cell Functions ◽  
Flame Emission ◽  
Monovalent Ions ◽  
In Cells

AbstractMonovalent ions, sodium in particular, are involved in fundamental cell functions, such as water balance and electric processes, intra- and intercellular signaling, cell movement, pH regulation and metabolite transport into and out of cells. Fluorescent probes are indispensable tools for monitoring intracellular sodium levels in single living cells in heterogeneous cell populations and tissues. Since the fluorescence of sodium-sensitive dyes in cells is significantly different from that in an aqueous solution, the fluorescence signal is calibrated in situ by changing the concentration of extracellular sodium in the presence of ionophores, making the membrane permeable to sodium and equilibrating its intra- and extracellular concentrations. The reliability of this calibration method has not been well studied. Here, we compare the determinations of the intracellular sodium concentration by flame emission photometry and flow cytometry using the Na+-sensitive probe Asante Natrium Green-2 (ANG). The intracellular Na+ concentration was altered using known ionophores or, alternatively, by blocking the sodium pump with ouabain or by causing cell apoptosis with staurosporine. The use of U937 cells cultured in suspension allowed the fluorometry of single cells by flow cytometry and flame emission analysis of samples checked for uniform cell populations. It is revealed that the ANG fluorescence of cells treated with ionophores is approximately two times lower than that in cells with the same Na+ concentration but not treated with ionophores. Although the mechanism is still unknown, this effect should be taken into account when a quantitative assessment of the concentration of intracellular sodium is required. Sodium sensitive fluorescent dyes are widely used at present, and the problem is practically significant.

Critical Evaluation of a New Flame Emission Analysis System, the "Klina Flame"

1972 ◽  
Vol 18 (9) ◽  
pp. 1005-1008 ◽  
Author(s):  
Richard J Schlesinger ◽  
Raymond A Lesonsky ◽  
Robert Lottritz
Keyword(s):  
Critical Evaluation ◽  
Paired Data ◽  
Serum Samples ◽  
Emission Analysis ◽  
Long Term Stability ◽  
Flame Emission ◽  
Analysis System ◽  
Instrumental Methods ◽  
Predictable Variation

Abstract Recently there have been several advances in instrumental methods for the flame emission analysis of electrolyte cations of clinical significance. Greater precision and increased ease of operation are among today’s clinical requirements. This study compares a new automated flame photometer, "Klina Flame" (Beckman Instruments) with a "Techtron-AA5" atomic absorption emission unit (Varian Instruments) and an "IL 143" flame system (Instrumentation Laboratory). Day-to-day variation between paired data over a three-month period showed comparable and explainable similarities and variations between the three instruments. Results of lithium determinations gave favorable comparison statistics. Long-term stability on all the systems were generally comparable. A comparison of manual vs. automatic modes of dilution with serum samples of various viscosities generally gave predictable variation for instruments of similar quality. The piston dilutor feature of the Klina system seems to prevent the variability seen at higher viscosities when the IL system is used, which has a peristaltic dilutor.

Simplified Slurry Method for Direct Flame Emission Determinations of Dietary Salt in Processed Meat

1986 ◽  
Vol 40 (2) ◽  
pp. 233-238 ◽  
Author(s):  
M. D. Wichman ◽  
R. Fietkau ◽  
R. C. Fry
Keyword(s):  
Processed Meat ◽  
Ground Meat ◽  
Dietary Salt ◽  
Emission Analysis ◽  
Processed Meats ◽  
Household Food ◽  
Flame Emission ◽  
Dry Ashing ◽  
Slurry Method

The rapid, direct slurry atomization flame emission procedure for Na and K determination is shown to give accurate results for a variety of processed meats including bologna, salami, sausage, ham, bacon, and pizza products. Wet and dry ashing methods are found to be unnecessary in this application. The sample preparation scheme for the elements Na and K has been even further simplified over our previous slurry atomization report (Ref. 1) by elimination of the sonic/cavitational homogenizer in favor of an ordinary household food processor (meat grinder). Ground meat or pizza is simply suspended by being shaken in some deionized water followed immediately by direct flame emission analysis of the suspension using a slurry nebulizer and a high-solids premixed natural-gas/air flame. A simple clinical-type filter photometer is evaluated as a substitute for the grating instrument previously used for the determination of dietary salt in processed meat by slurry atomization.

Application of a Vidicon Spectrometer for Simultaneous Flame-Emission Analysis for Sodium and Potassium in Serum

1974 ◽  
Vol 20 (11) ◽  
pp. 1422-1430 ◽  
Author(s):  
Thomas E Cook ◽  
Michael J Milano ◽  
Harry L Pardue
Keyword(s):  
Emission Analysis ◽  
New Approach ◽  
Flame Emission ◽  
Coefficients Of Variation ◽  
Silicon Target ◽  
Visible Spectra ◽  
Hydrogen Flame ◽  
Basic Studies ◽  
Sodium And Potassium

Abstract We describe a unique new approach to analytical spectroscopy, which makes use of a silicon-target vidicon tube to rapidly and repetitively scan ultraviolet and visible spectra. We discuss the concept and characteristics of the vidicon spectrometer and illustrate its application to simultaneous determination of sodium and potassium in serum by flame photometry. Average values of coefficients of variation for sodium and potassium are 1.1% and 1.7%, respectively. Recoveries of sodium and potassium added to sera were 99.5% and 99.8%, respectively. When the same burner and flame conditions were used with the vidicon spectrometer and a conventional flame photometer, deviations among results were well within ±3 mmol/liter for sodium and 0.2 mmol/liter for potassium. A bias is observed for both elements when results obtained with an air-hydrogen flame are compared with results obtained with an air-propane flame. Our data illustrate that the vidicon spectrometer is a viable tool for both routine applications and basic studies of effects of flame conditions on two or more elements simultaneously.

The Role of Flame Shape and Burner Configuration in Emission Analysis

1969 ◽  
Vol 23 (6) ◽  
pp. 597-600 ◽  
Author(s):  
S. R. Koirtyohann ◽  
E. E. Pickett
Keyword(s):  
Atomic Absorption ◽  
Detection Limits ◽  
Optical Systems ◽  
Noise Levels ◽  
Simple Derivation ◽  
Emission Analysis ◽  
Flame Emission ◽  
Lower Detection ◽  
Flame Shape

A simple derivation can show that the effect of flame shape, multiple flames, and multi-pass optical systems should be the same for flame emission intensity as for atomic absorption response. Experimental evidence confirms the prediction and shows that the steps used to increase atomic absorption response are directly applicable to emission measurements. Increased emission intensities reduce noise levels and lower detection limits by lowering the required electrical gain and by permitting the use of narrower spectrometer slits. The improvement in detection limits from the use of narrow slits depends on the background structure in the vicinity of the line. Three cases are considered in detail.

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