Ammonium sulfate: Modified French method of production

1929 ◽  
Vol 6 (1) ◽  
pp. 43
Keyword(s):  
Ammonium Sulfate ◽  
Method Of Production

The Coagulation Abnormalities in Systemic Lupus Erythematosus

1968 ◽  
Vol 20 (03/04) ◽  
pp. 457-464 ◽  
Author(s):  
L Gonyea ◽  
R Herdman ◽  
R. A Bridges
Keyword(s):  
Systemic Lupus Erythematosus ◽  
Ammonium Sulfate ◽  
Lupus Erythematosus ◽  
Anticoagulant Activity ◽  
Species Specificity ◽  
Sensitive Assay ◽  
Systemic Lupus ◽  
Coagulation Abnormalities

SummaryAn anticoagulant occurring in 4 of 6 patients with SLE has been demonstrated by a sensitive assay utilizing an ammonium sulfate fraction of serum. The anticoagulant functions as an inhibitor of the activation of prothrombin. No species specificity was demonstrable. The inhibitor behaves clinically and chromatographically as an immunoglobulin, although an attempt to demonstrate directly the antibody nature of the inhibitor was not successful.A severe, apparently independent, decrease in the level of prothrombin was observed in the patient with hemorrhagic symptoms. In contrast to the anticoagulant activity, the low prothrombin has persisted during treatment.

Extraction and Partial Purification of Protease from Bilimbi (Averrhoa bilimbi L.)

2013 ◽  
Vol 10 (2) ◽  
pp. 29
Author(s):  
Normah Ismail ◽  
Nur' Ain Mohamad Kharoe
Keyword(s):  
Molecular Weight ◽  
Protein Content ◽  
Ammonium Sulfate ◽  
Molecular Weight Distribution ◽  
Weight Distribution ◽  
Protease Activity ◽  
Protein Concentration ◽  
Temperature Stability ◽  
Partial Purification ◽  
Ammonium Sulfate Precipitation

Unripe and ripe bilimbi (Averrhoa bilimbi L.) were ground and the extracted juices were partially purified by ammonium sulfate precipitation at the concentrations of 40 and 60% (w/v). The collected proteases were analysed for pH, temperature stability, storage stability, molecular weight distribution, protein concentration and protein content. Protein content of bilimbi fruit was 0.89 g. Protease activity of both the unripe and ripe fruit were optimum at pH 4 and 40°C when the juice were purified at 40 and 60% ammonium sulfate precipitation. A decreased in protease activity was observed during the seven days of storage at 4°C. Molecular weight distribution indicated that the proteases protein bands fall between IO to 220 kDa. Protein bands were observed at 25, 50 and 160 kDa in both the unripe and ripe bilimbi proteases purified with 40% ammonium sulfate, however, the bands were more intense in those from unripe bilimbi. No protein bands were seen in proteases purified with 60% ammonium sulfate. Protein concentration was higher for proteases extracted with 40% ammonium sulfate at both ripening stages. Thus, purification using 40% ammonium sulfate precipitation could be a successful method to partially purify proteases from bilimbi especially from the unripe stage. 

Effect of Methionine and Ammonium Sulfate upon Performance of Ruminants Fed High Corn Rations

1973 ◽  
Vol 36 (6) ◽  
pp. 1186-1190 ◽  
Author(s):  
K. K. Bolsen ◽  
Walter Woods ◽  
Terry Klopfenstein
Keyword(s):  
Ammonium Sulfate

Pengaruh Penambahan Molases Pada Kulit Pisang Kapendis Untuk Media Produksi Xilanase B.Stearothermophillus DSM 22

2019 ◽  
Vol 15 (3) ◽  
Author(s):  
Trismillah
Keyword(s):  
Enzyme Activity ◽  
Ammonium Sulfate ◽  
Banana Peel ◽  
Partial Purification ◽  
Temperature Conditions ◽  
Cavendish Banana ◽  
Working Volume ◽  
Initial Ph ◽  
Xylanase Enzyme

Cavendish banana peel can be used as a substitute for the expensive xylan, while molasses than as a source of carbon as well as nitrogen, minerals and nutrients needed for the growth of microbes that can produce the enzyme. Xylanase produced from Bacillus stearothermopillus DSM 22, using media cavendish banana peels with the addition of molasses 1%, 2%, and 3%. Fermentation is done in a shaker incubator at 550C temperature conditions, initial pH 8, and 250 rpm agitation. The result showed the highest enzyme activity of 4,14 ± 0,16 U/mL min., on the addition 2% molasses after 24 hours. Further fermentation carried out in the fermenter working volume of 3.5 liters, with the condition of temperature 550C, pH 8, aeration 1 vvm, agitation 250 rpm, the highest spesific enzyme of activity of 51,62 ± 0,16 U/mg after 24 hours. Partial purification of xylanase enzyme fermentation is done with the results of microfiltration, ultrafiltration, ammonium sulfate (0-80%) and dialysis. There is an increase in the purity of the enzyme at each stage of purification, the highest purity on dialysis 3.23 times of crude enzymes.Kulit buah pisang kapendis dapat digunakan sebagai pengganti xilan yang harganya mahal, sementara molases selain sebagai sumber karbon serta nitrogen, mineral dan nutrisi dibutuhkan untuk pertumbuhan mikroba yang dapat menghasilkan enzim. Xilanase yang dihasilkan dari Bacillus stearothermopillus DSM 22, menggunakan media kulit pisang kapendis dengan penambahan molase 1%, 2%, dan 3%. Fermentasi dilakukan dalam shaker inkubator pada temperatur 550C, pH awal 8, dan agitasi 250 rpm. Hasilnya menunjukkan aktivitas enzim tertinggi 4,14 ± 0,16 U/mL min., pada penambahan 2% molases setelah 24 jam. Selanjutnya fermentasi dilakukan di dalam fermentor, volume kerja dari 3,5 liter, dengan kondisi temperatur 550C, pH 8, aeration 1 vvm, agitasi 250 rpm, aktivitas spesifik tertinggi 51,62 ± 0,16 U/mg setelah 24 jam. Pemurnian parsial fermentasi enzim xilanase dilakukan dengan hasil mikrofiltrasi, ultrafiltrasi, amonium sulfat (0-80%) dan dialisis. Ada peningkatan kemurnian enzim pada setiap tahap pemurnian, kemurnian tertinggi pada dialisis 3,23 kali dari enzim kasar.Keywords: Xylanase, B. stearothermophillus DSM 22, Cavendish banana peel, molasses, enzyme activity

Decision-Making Practice When Choosing the Method of Production of Ceramic Bricks

2020 ◽  
Vol 783 (8) ◽  
pp. 4-8
Author(s):  
Syromyasov V.A. ◽  
◽  
Vakalova T.V. ◽  
Storozhenko G.I. ◽  
◽  
...  
Keyword(s):  
Decision Making ◽  
Method Of Production

Influence of formulations of nitrogen fertilizes on oil flax productivity under conditions of western forest-steppe

2018 ◽  
pp. 108-114
Keyword(s):  
Ammonium Sulfate ◽  
Mineral Fertilizer ◽  
Flaxseed Oil ◽  
Climatic Conditions ◽  
Nitrogen Fertilizers ◽  
Forest Steppe ◽  
Simultaneous Application ◽  
Traditional System ◽  
Carpathian Region ◽  
High Yields

The results of studies on the influence of forms and doses of nitrogen fertilizers (ammonium nitrate, urea, carbamide-ammonia mixture, ammonium sulfate) on the productivity of oil flax under the conditions of the western forest-steppe are given. In the traditional system of fertilizer, oil flax is not able to provide high yields and quality products. An effective solution to this is optimization of plant nutrition due to its balance, which promotes intensive plant development, makes it possible to actively and efficiently intervene in the process of harvesting and its quality. Therefore, determining the optimal fertilizer rate for oil flax in specific soil-climatic conditions is important for obtaining stable and high yields of culture. The purpose of our research was to determine the contribution of doses and forms of nitrogen fertilizers to the productivity of flaxseed oil in the soil-climatic conditions of the western forest-steppe. The research was carried out in 2016-2018 at the experimental field of the Institute of Agriculture of the Carpathian region of NAAS in five-point crop rotation with the total area of the plant growing on a gray forest surface glued type of soil In general, four forms of nitrogen fertilizers were studied: ammonia, nitrate, carbamide-ammonia mixture, urea and ammonium sulfate, which were added in doses: N30; N45 one time; and N30 + N15 under pre-sowing cultivation and in the "fir tree" phase against the background of P30K60. The total number of variants was 16. The object of research was a variety of flax oilseed crops of breeding of the Institute of Oilseeds of NAAS. The seed sowing rate is 6 mln similar seed per hectare. According to the results of three-year studies, the effect of intensification of mineral nitrogen feed and various forms of nitrogen fertilizers on the indices of the elements of productivity and yield of flaxseed oil has been established. In particular, it was found that their use had little effect on the density change of plant stem growth. The use of nitrogen fertilizers in the dose N45 on the background of P30K60 improved plant survival (killed during the vegetation of 0.33-0.42 million pp./ha, which is 5.2-6.3 % for control, where the death observed 0.58 million ppm/ha or 8.4 %)) compared to the N30 dose (0.39-0.49 million pcs/ha). In addition, the use of the dose N45 separately (N30 under cultivation + N15 in the phase of the "fir tree") also improved the survival rate of plants (5.2-5.8 % of dead plants) compared with the simultaneous application of N45 cultivation (5.6-7.1 %) The percentage of deaths grew in control (without fertilizers) - 8.4 % against the background of P30K60 - 7.0 %. The application of various forms and doses of nitrogen fertilizers led to an increase in the control of the elements of productivity. The introduction of N45 against the background of P30K60 contributed to an increase in the number of boxes on plants, according to the variants of fertilizers, this index varied from 14.6 to 18.3 pieces per plant (10.9 - on the control, 12.9 pcs/roll - on the РК background), increasing the amount of seeds per plant by 12.7 pc. per plant, a mass of 1000 seeds per 0.2-0.3 g compared with the control and 0.1-0.2 g compared with the LCD background, where this figure was 6.8 g. On average, over the years of research, the highest productivity of flax seed of oilseed Vodograi (2,27 and 2,48 t / ha) was obtained provided the mineral fertilizer N15P30K60 was applied for cultivation in combination with carbamide feed in the phase of "fir tree" at a dose of N15. When using the nitrogen component in the form of KAS, the gain to control was 1.45 t / ha, to the background - 1.12 t/ha. In control and against the background, this indicator was 1.04 and 1.36 t/ha respectively. When using other forms of nitrogen fertilizers on the background of P30K60, lower yields of flaxseed crops were obtained (1.37-1.38 t/ha). The introduction of the N45 separately (N30 under cultivation + N15 into the "fir tree" phase) generally led to higher performance than a single application for cultivation. Among the studied forms of fertilizers, urea and carbamide-ammonia also caused a slightly higher percentage of fiber content in flax stems, however, it was within the experimental error and was not significantly increased.

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