One-Step Synthesis of Nanosized and Stable Amino-Functionalized Calcium Phosphate Particles for DNA Transfection

2013 ◽  
Vol 25 (18) ◽  
pp. 3667-3674 ◽  
Author(s):  
Babak Mostaghaci ◽  
Brigitta Loretz ◽  
Robert Haberkorn ◽  
Guido Kickelbick ◽  
Claus-Michael Lehr
Keyword(s):  
Calcium Phosphate ◽  
Dna Transfection ◽  
Calcium Phosphate Particles ◽  
One Step

One-step formation of a double Pickering emulsion via modulation of the oil phase composition

2018 ◽  
Vol 9 (8) ◽  
pp. 4508-4517 ◽  
Author(s):  
Qijun Ruan ◽  
Lihua Zeng ◽  
Jiaoyan Ren ◽  
Xiaoquan Yang
Keyword(s):  
Phase Composition ◽  
Calcium Phosphate ◽  
Pickering Emulsion ◽  
Food Grade ◽  
Calcium Phosphate Particles ◽  
One Step

A facile one-step emulsification strategy was developed to generate a food-grade W/O/W double Pickering emulsion by using corn-peptide-functionalized calcium phosphate particles (CP-CaP) as emulsifier.

A protocol to develop crack-free biomimetic coatings on Ti6Al4V substrates

2007 ◽  
Vol 22 (6) ◽  
pp. 1593-1600 ◽  
Author(s):  
Sahil Jalota ◽  
Sutapa Bhaduri ◽  
Sarit B. Bhaduri ◽  
A. Cuneyt Tas
Keyword(s):  
Water Treatment ◽  
Calcium Phosphate ◽  
Alkali Treatment ◽  
Sodium Titanate ◽  
Calcium Phosphate Coating ◽  
First Case ◽  
Phosphate Coatings ◽  
Biomimetic Coating ◽  
One Step ◽  
Calcium Phosphate Coatings

Biomimetic coating of titanium and related alloys with carbonated apatitic calcium phosphate is an important area of research in implantology. While this paper specifically refers to coating Ti6Al4V, the results are valid with other related alloys as well. One step in the protocol involves an intermediate alkali treatment of Ti6Al4V to form a sodium titanate layer on the alloy surface. This pretreatment enhances the formation of the coating from simulated body fluid (SBF) solutions. Many papers in the biomimetic coating literature demonstrate the presence of cracks in coatings, irrespective of the SBF compositions and placement of the substrates. The presence of cracks may result in degradation and delamination of coatings. To the best of our knowledge, this issue remains unresolved. Therefore, the aim of this study was: (i) to examine and understand the reasons for cracking and (ii) based on the results, to develop a protocol for producing crack-free apatitic calcium phosphate coatings on Ti6Al4V substrates. In this study, the authors focused their attention on the alkali treatment procedure and the final drying step. It is hypothesized that these two steps of the process affect the crack formation the most. In the first case, the surfaces of alkali-treated substrates were examined with/without water-soaking treatment before immersing in SBF. This water treatment modifies the sodium titanate surface layer. In the second case, two different drying techniques (after soaking in SBF) were used. In one procedure, the coated substrates were dried rapidly, and in the other they were dried slowly. It was observed that the water treatment, irrespective of the drying method, provides a surface, which on subsequent soaking in SBF forms a crack-free apatitic calcium phosphate coating. Based on these results, the authors suggest a protocol incorporating a water-soaking treatment after the alkali treatment and prior to the SBF soaking treatment to obtain crack-free coatings.

scholarly journals The study of the microstructure of cheese before and after vacuum drying

2020 ◽  
Vol 10 (4) ◽  
pp. 6007-6014
Keyword(s):  
Calcium Phosphate ◽  
Surface Shape ◽  
Vacuum Drying ◽  
Protein Matrix ◽  
Fat Globules ◽  
Before And After ◽  
Calcium Phosphate Particles ◽  
Structure State ◽  
Cheese Microstructure ◽  
Scanning Electron

Scanning electron microscope allowed us to get screens of different cheese microstructure that form a base for further investigation of a cheese structure state before and after the process of drying and for their comparison. Any cheese structure presents a matrix of proteins penetrated with moisture capillaries; fat globules are located both inside the protein matrix and on a cheese surface. Shape of capillaries is either round or oval. Capillaries vary in size and number that has an impact on the cheese pattern which is described by hole and void shapes and order. Electron microscopy was also used for detecting deposition of calcium phosphate. Particles of calcium phosphate changed in size, before drying they were 10–12 µm, and after drying they reached 20–30 µ. These particles concentrate in the dried cheese and agglomerate into larger particles. The most concentrated calcium phosphate proportion was found in pores and micro-voids of the dry cheese. As for mature cheese samples, calcium lactate was established as well.

scholarly journals Sedimentation Study of Biphasic Calcium Phosphate Particles

2007 ◽  
pp. 365-368
Author(s):  
Ahmed Fatimi ◽  
Jean Francois Tassin ◽  
Monique Aselo V. Axelos ◽  
Pierre Weiss
Keyword(s):  
Calcium Phosphate ◽  
Biphasic Calcium Phosphate ◽  
Calcium Phosphate Particles

Microfiltration performance: physicochemical aspects of whey pretreatment

1995 ◽  
Vol 62 (2) ◽  
pp. 269-279 ◽  
Author(s):  
Genevieve Gesan ◽  
Georges Daufin ◽  
Uzi Merin ◽  
Jean-Pierre Labbe ◽  
Auguste Quemerais
Keyword(s):  
Calcium Phosphate ◽  
Membrane Surface ◽  
Physicochemical Process ◽  
High Shear ◽  
Lower Content ◽  
Phosphate Ions ◽  
Complex Lipid ◽  
Ionic Calcium ◽  
Calcium Phosphate Particles ◽  
Soluble Calcium

SUMMARYClarification of whey by microfiltration (MF) can be achieved after appropriate pretreatment of the feed. A control pretreatment consists of a physicochemical process comprising increased ionic calcium and pH accompanied by heat (50 °C, 15 min) to cause aggregation of complex lipid–calcium phosphate particles, which are then separated by MF. This pretreatment process was modified by increasing the temperature to 55 °C and by maintaining the pH constant during heat treatment. This modification resulted in larger calcium phosphate particles and a lower content of soluble calcium and phosphate ions. As a consequence, a longer period of MF operation, better whey clarification and lower calcium and phosphate content of the filtrate were achieved. This suggests that a loosely structured deposit was formed on the membrane surface which was less resistant to filtration than that resulting from the control pretreatment. During MF, it was necessary to avoid zones of high shear in the retentate compartment that might cause physical alteration of the aggregates.

Adsorption of milk proteins on to calcium phosphate particles

2013 ◽  
Vol 394 ◽  
pp. 458-466 ◽  
Author(s):  
Lucile Tercinier ◽  
Aiqian Ye ◽  
Skelte Anema ◽  
Anne Singh ◽  
Harjinder Singh
Keyword(s):  
Calcium Phosphate ◽  
Milk Proteins ◽  
Calcium Phosphate Particles
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