scholarly journals For Your Fine Chemical Needs

1947 ◽  
Vol 25 (17) ◽  
pp. ibc
Keyword(s):  
Fine Chemical

scholarly journals Refactoring of a synthetic raspberry ketone pathway with EcoFlex

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Simon J. Moore ◽  
Yonek B. Hleba ◽  
Sarah Bischoff ◽  
David Bell ◽  
Karen M. Polizzi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Synthetic Biology ◽  
Combinatorial Libraries ◽  
Value Added ◽  
Microtiter Plate ◽  
Fine Tuning ◽  
Golden Gate ◽  
E Coli ◽  
Fine Chemical ◽  
Raspberry Ketone

Abstract Background  A key focus of synthetic biology is to develop microbial or cell-free based biobased routes to value-added chemicals such as fragrances. Originally, we developed the EcoFlex system, a Golden Gate toolkit, to study genes/pathways flexibly using Escherichia coli heterologous expression. In this current work, we sought to use EcoFlex to optimise a synthetic raspberry ketone biosynthetic pathway. Raspberry ketone is a high-value (~ £20,000 kg−1) fine chemical farmed from raspberry (Rubeus rubrum) fruit. Results  By applying a synthetic biology led design-build-test-learn cycle approach, we refactor the raspberry ketone pathway from a low level of productivity (0.2 mg/L), to achieve a 65-fold (12.9 mg/L) improvement in production. We perform this optimisation at the prototype level (using microtiter plate cultures) with E. coli DH10β, as a routine cloning host. The use of E. coli DH10β facilitates the Golden Gate cloning process for the screening of combinatorial libraries. In addition, we also newly establish a novel colour-based phenotypic screen to identify productive clones quickly from solid/liquid culture. Conclusions  Our findings provide a stable raspberry ketone pathway that relies upon a natural feedstock (L-tyrosine) and uses only constitutive promoters to control gene expression. In conclusion we demonstrate the capability of EcoFlex for fine-tuning a model fine chemical pathway and provide a range of newly characterised promoter tools gene expression in E. coli.

Tuning the catalytic performance for the semi-hydrogenation of alkynols by selectively poisoning the active sites of Pd catalysts

2019 ◽  
Vol 21 (15) ◽  
pp. 4143-4151 ◽  
Author(s):  
Shanjun Mao ◽  
Bowen Zhao ◽  
Zhe Wang ◽  
Yutong Gong ◽  
Guofeng Lü ◽  
...  
Keyword(s):  
Active Sites ◽  
Catalytic Performance ◽  
Pd Catalysts ◽  
Fine Chemical

Semi-hydrogenation of alkynols to alkenols with Pd-based catalysts is of great significance in fine chemical industries.

scholarly journals THE FINE CHEMICAL INDUSTRY.

The Lancet ◽  
1921 ◽  
Vol 197 (5087) ◽  
pp. 457
Author(s):  
STAFFORDALLENANDSONSLTD
Keyword(s):  
Chemical Industry ◽  
Fine Chemical
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