Rapid Microwave-Assisted CNBr Cleavage of Bead-Bound Peptides

Su Seong Lee; Jaehong Lim; Junhoe Cha; Sylvia Tan; James R. Heath

doi:10.1021/cc800113d

Rapid Microwave-Assisted CNBr Cleavage of Bead-Bound Peptides

Journal of Combinatorial Chemistry ◽

10.1021/cc800113d ◽

2008 ◽

Vol 10 (6) ◽

pp. 807-809 ◽

Cited By ~ 11

Author(s):

Su Seong Lee ◽

Jaehong Lim ◽

Junhoe Cha ◽

Sylvia Tan ◽

James R. Heath

Keyword(s):

Microwave Assisted ◽

Cnbr Cleavage

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Microwave-assisted hydrodistillation of essential oil from cherry laurel (Prunus laurocerasus) leaves

Planta Medica ◽

10.1055/s-0029-1234797 ◽

2009 ◽

Vol 75 (09) ◽

Author(s):

I Stanisavljević ◽

M Lazić ◽

N Radulović ◽

V Veljković

Keyword(s):

Essential Oil ◽

Microwave Assisted ◽

Cherry Laurel ◽

Prunus Laurocerasus

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Microwave assisted glycosylation for determining the absolute configuration of carbohydrates in plant glycosides

Planta Medica ◽

10.1055/s-0032-1321184 ◽

2012 ◽

Vol 78 (11) ◽

Author(s):

KP Manfredi ◽

H Frosch

Keyword(s):

Absolute Configuration ◽

Microwave Assisted ◽

The Absolute

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Preparation and Characterization of NH2-Terminal Fibrinogen Bβ Fragments from N-DSK of Human Fibrinogen

Thrombosis and Haemostasis ◽

10.1055/s-0038-1660999 ◽

1984 ◽

Vol 51 (01) ◽

pp. 016-021 ◽

Cited By ~ 2

Author(s):

S Birken ◽

G Agosto ◽

B Lahiri ◽

R Canfield

Keyword(s):

High Performance ◽

Gel Filtration ◽

Ion Exchange Chromatography ◽

Exchange Chromatography ◽

Reverse Phase ◽

Human Fibrinogen ◽

Human Volunteers ◽

Cnbr Cleavage ◽

Two Peaks

SummaryIn order to investigate the early release of NH2-terminal plasmic fragments from the Bβ chain of fibrinogen, substantial quantities of Bβ 1-42 and Bβ 1-21 are required as immunogens, as radioimmunoassay standards and for infusion into human volunteers to determine the half-lives of these peptides. Towards this end methods that employ selective proteolytic cleavage of these fragments from fibrinogen have been developed. Both the N-DSK fragment, produced by CNBr cleavage of fibrinogen, and Bβ 1-118 were employed as substrates for plasmin with the finding of higher yields from N-DSK. Bβ 1-42 and Bβ 1-21 were purified by gel filtration and ion-exchange chromatography on SP-Sephadex using volatile buffers. When the purified preparation of Bβ 1-42 was chromatographed on reverse-phase high performance liquid chromatography, two peaks of identical amino acid composition were separated, presumably due either to pyroglutamate or to amide differences.

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