Parallel Synthesis and Yeast Growth Inhibition Screening of Succinamic Acid Libraries

2007 ◽  
Vol 9 (4) ◽  
pp. 635-643 ◽  
Author(s):  
Pedro Serrano ◽  
Josefina Casas ◽  
Amadeu Llebaria ◽  
Martine Zucco ◽  
Gilbert Emeric ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Parallel Synthesis ◽  
Yeast Growth ◽  
Succinamic Acid ◽  
Yeast Growth Inhibition

Xylosylation as an effective means for reducing yeast growth inhibition by 2-phenylethanol

2013 ◽  
Vol 53 (9) ◽  
pp. 792-795 ◽  
Author(s):  
Haojun Zhang ◽  
Régis Fauré ◽  
Jean M. François ◽  
Philippe J. Blanc ◽  
Gustavo M. de Billerbeck
Keyword(s):  
Growth Inhibition ◽  
Effective Means ◽  
Yeast Growth ◽  
Yeast Growth Inhibition

scholarly journals RACK1 Interacts with E1A and Rescues E1A-induced Yeast Growth Inhibition and Mammalian Cell Apoptosis

2001 ◽  
Vol 276 (29) ◽  
pp. 27026-27033 ◽  
Author(s):  
Nianli Sang ◽  
Anna Severino ◽  
Patrizia Russo ◽  
Alfonso Baldi ◽  
Antonio Giordano ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Mammalian Cell ◽  
Cell Apoptosis ◽  
Yeast Growth ◽  
Yeast Growth Inhibition

HIV-1 Nef Alleles Show Differential Activation of Hematopoietic Cell Kinase in a Yeast Model System.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 3107-3107
Author(s):  
Ronald P. Trible ◽  
Thomas E. Smithgall
Keyword(s):  
Growth Inhibition ◽  
Mammalian Cells ◽  
Sh2 Domain ◽  
Model System ◽  
Hematopoietic Cell ◽  
Yeast Growth ◽  
High Affinity ◽  
Hematopoietic Cell Kinase ◽  
Aids Progression ◽  
Hiv 1

Abstract Hematopoietic cell kinase (Hck) is a member of the Src family of non-receptor protein-tyrosine kinases that is expressed strongly in macrophages, an important target cell type for human immunodeficiency virus (HIV). The HIV Nef protein, a critical AIDS progression factor, binds Hck with unusually high affinity and induces Hck activation. Nef-mediated Hck activation has been proposed to trigger signaling pathways important for the establishment of HIV infection and subsequent progression to AIDS. Previous studies have utilized sequence alignments and binding assays to predict the functionality of Hck-Nef interactions, but these studies do not directly investigate the ability of Nef alleles to activate Hck. To better address this issue, we have created a model system in Saccharomyces cerevisiae based on previous findings that ectopic expression of c-Src arrests yeast growth in a kinase-dependent fashion. To establish the utility of this system for Hck and Nef, we first created a Hck variant containing a C-terminal sequence modified to encode the high-affinity SH2-domain binding sequence Tyr-Glu-Glu-Ile (HckYEEI). This modified sequence allows for autophosphorylation of the new tail and the subsequent intramolecular binding to the Hck SH2 domain. The resulting HckYEEI molecule models the physiologically down-regulated (inactive) form of Hck in structure and function. Unlike wild-type Hck, HckYEEI showed little kinase activity and failed to induce growth suppression in yeast. Importantly, co-expression with the Nef-SF2 allele activated HckYEEI, resulting in growth arrest. This effect was dependent on the conserved Nef proline-rich motif essential for Hck binding through its SH3 domain. However, Nef-SF2 did not activate FynYEEI, consistent with our previous data that the Fyn tyrosine kinase is not activated by Nef in mammalian cells. We then tested several additional laboratory HIV-1 Nef alleles for their ability to activate HckYEEI. Consensus, Lai, NL4.3, SF2, and YU-2 Nef alleles activated HckYEEI to varying degrees, as reflected by yeast growth inhibition. In contrast, Nef-Eli failed to suppress growth in the presence of HckYEEI, consistent with its inability to bind the Hck SH3 domain in vitro. Growth inhibition paralleled tyrosine phosphorylation of yeast proteins on anti-phosphotyrosine immunoblots. The differential effects of these Nef alleles on Hck activation correlated closely with previous results in mammalian cells. Taken together, these studies validate the use of yeast to reconstitute Nef-Hck interactions in a system amenable to screens of clinical Nef isolates, which may have predictive value in terms of AIDS progression.

scholarly journals Nitrogen source-dependent inhibition of yeast growth by glycine and its N-methylated derivatives

2019 ◽  
Vol 113 (3) ◽  
pp. 437-445
Author(s):  
Tomas Linder
Keyword(s):  
Growth Inhibition ◽  
Nitrogen Source ◽  
Minimal Medium ◽  
Kluyveromyces Lactis ◽  
Nitrogen Sources ◽  
Growth Efficiency ◽  
Sole Source ◽  
Yeast Growth ◽  
Glycine Species ◽  
Dependent Inhibition

Abstract The effect of nitrogen source on the inhibitory properties of glycine and its N-methylated derivatives N-methylglycine (sarcosine), N,N-dimethylglycine, N,N,N-trimethylglycine (glycine betaine) on yeast growth was investigated. On solid minimal medium, all four glycine species completely or partially inhibited growth of Kluyveromyces lactis, Komagataella pastoris, Ogataea arabinofermentans, Spathaspora passalidarum and Yamadazyma tenuis at concentrations 5–10 mM when 10 mM NH4Cl was the sole source of nitrogen. If NH4Cl was substituted by sodium L-glutamate as the sole source of nitrogen, obvious growth inhibition by glycine and its N-methylated derivatives was generally not observed in any of these species. No obvious growth inhibition by any of the glycine species at a concentration of 10 mM was observed in Cyberlindnera jadinii, Lipomyces starkeyi, Lodderomyces elongisporus, Scheffersomyces stipitis or Yarrowia lipolytica on solid minimal medium irrespective of whether the nitrogen source was NH4Cl or sodium L-glutamate. Growth inhibition assays of K. pastoris in liquid minimal medium supplemented with increasing concentrations of N,N-dimethylglycine demonstrated inhibitory effects for nine tested nitrogen sources. In most cases, N,N-dimethylglycine supplementation caused a decrease in growth efficiency that appeared to be proportional to the concentration of N,N-dimethylglycine. The biological relevance of these results is discussed.

Effect of Spent Sulfite Liquor on Growth and Ethanol Fermentation Efficiency of Saccharomyces Ellipsoideus

1981 ◽  
Vol 16 (1) ◽  
pp. 91-102 ◽  
Author(s):  
N. Kosaric ◽  
K.K. Ho ◽  
Z. Duvnjak
Keyword(s):  
Growth Inhibition ◽  
Ethanol Fermentation ◽  
Growth Media ◽  
Complete Elimination ◽  
Yeast Growth ◽  
Fermentation Efficiency ◽  
Growth Inhibiting ◽  
Spent Sulfite Liquor

Abstract The objective of this study was to evaluate the extent of inhibition of yeast growth and alcohol biosynthesis when spent sulfite liquor (SSL) was incorporated into growth media. The liquor was pretreated by boiling, steam stripping or aeration in order to eliminate the factors causing growth inhibition. While these treatments did reduce the growth inhibiting effects of SSL, complete elimination of inhibition was not possible.

Kinetics of yeast growth: inhibition-threshold substrate concentrations

1975 ◽  
Vol 21 (7) ◽  
pp. 994-1003 ◽  
Author(s):  
Murray Men-chung Tseng ◽  
Morris Wayman
Keyword(s):  
Acetic Acid ◽  
Growth Rates ◽  
Substrate Concentration ◽  
The Other ◽  
Yeast Growth ◽  
Inhibition Concentration ◽  
Total Inhibition ◽  
Yeast Growth Inhibition ◽  
Kinetics Of ◽  
Substrate Concentrations

Two Candida species and a Saccharomyces were grown on ethyl alcohol, acetic acid, ethyl acetate, and 1-butanol, and their growth rates at a series of concentrations of each substrate are reported. Substrate concentrations higher than a characteristic threshold substrate concentration S0 inhibited growth in accordance with a new relationship:[Formula: see text]where i is an inhibition constant and the other symbols have their usual meanings. Complete inhibition, that is, no growth, takes place when S = Si, the total inhibition concentration. Values of S0, Si, and i were calculated for the examples studied. Also discussed are the observed lag periods and also the lowered yields at high substrate concentrations.

Light-induced increase in the contents of ferulic and diferulic acids in cell walls of Avena coleoptiles: its relationship to growth inhibition by light

1994 ◽  
Vol 92 (2) ◽  
pp. 350-355 ◽  
Author(s):  
Kensuke Miyamoto ◽  
Junichi Ueda ◽  
Satomi Takeda ◽  
Kazuko Ida ◽  
Takayuki Hoson ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Cell Walls
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