Reprint of “Multiphase mixing characteristics in a microcarrier-based stirred tank bioreactor suitable for human mesenchymal stem cell expansion”

2017 ◽  
Vol 59 ◽  
pp. 266-275 ◽  
Author(s):  
Tanja A. Grein ◽  
Jasmin Leber ◽  
Miriam Blumenstock ◽  
Florian Petry ◽  
Tobias Weidner ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Expansion ◽  
Human Mesenchymal Stem Cell ◽  
Stirred Tank ◽  
Stirred Tank Bioreactor ◽  
Stem Cell Expansion ◽  
Mixing Characteristics

Effects of hypoxia on human mesenchymal stem cell expansion and plasticity in 3D constructs

2006 ◽  
Vol 207 (2) ◽  
pp. 331-339 ◽  
Author(s):  
Warren L. Grayson ◽  
Feng Zhao ◽  
Reza Izadpanah ◽  
Bruce Bunnell ◽  
Teng Ma
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Expansion ◽  
Human Mesenchymal Stem Cell ◽  
Stem Cell Expansion

Tunable Volumetric Density and Porous Structure of Spherical Poly-ε-caprolactone Microcarriers, as Applied in Human Mesenchymal Stem Cell Expansion

Langmuir ◽  
2017 ◽  
Vol 33 (12) ◽  
pp. 3068-3079 ◽  
Author(s):  
Jian Li ◽  
Alan Tin-Lun Lam ◽  
Jessica Pei Wen Toh ◽  
Shaul Reuveny ◽  
Steve Kah-Weng Oh ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Porous Structure ◽  
Cell Expansion ◽  
Human Mesenchymal Stem Cell ◽  
Stem Cell Expansion ◽  
Volumetric Density

The Scale-up of Human Mesenchymal Stem Cell Expansion and Recovery

2016 ◽  
pp. 91-125 ◽  
Author(s):  
Thomas R. J. Heathman ◽  
Qasim A. Rafiq ◽  
Karen Coopman ◽  
Alvin W. Nienow ◽  
Christopher J. Hewitt
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Expansion ◽  
Scale Up ◽  
Human Mesenchymal Stem Cell ◽  
Stem Cell Expansion

N-isopropylacrylamide-based thermoresponsive polyelectrolyte multilayer films for human mesenchymal stem cell expansion

2010 ◽  
Vol 26 (6) ◽  
pp. 1705-1713 ◽  
Author(s):  
Tianqing Liao ◽  
Maroun D. Moussallem ◽  
Junho Kim ◽  
Joseph B. Schlenoff ◽  
Teng Ma
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Expansion ◽  
Human Mesenchymal Stem Cell ◽  
Multilayer Films ◽  
Polyelectrolyte Multilayer ◽  
Stem Cell Expansion

Computations of Flow Environments in Medium-Scale Stirred-Tank Bioreactors for Stem Cell Expansion

2014 ◽  
Author(s):  
Petar Liovic ◽  
Ilija D. Šutalo ◽  
Laurence Meagher ◽  
George O. Lovrecz
Keyword(s):  
Stem Cell ◽  
Cell Fate ◽  
Cell Expansion ◽  
Model Development ◽  
Experimental Studies ◽  
Spatiotemporal Variability ◽  
Stirred Tank ◽  
Shear Stresses ◽  
Stirred Tank Bioreactor ◽  
Stem Cell Expansion

The flow in a New Brunswick Scientific (NBS (now Eppendorf)) 5 L stirred-tank bioreactor (STR) partially filled with 2.2 L of water and agitated at 60 rpm using a pitched-blade impeller is studied in this work, to determine the suitability of the configuration for expanding stem cell lines. Computational Fluid Dynamics (CFD) model development and testing in this work has found Large Eddy Simulation (LES) to be essential for model fidelity and for capturing spatiotemporal stress fluctuations. Stresses were at levels similar to or even higher than those known to damage stem cells or modulate their cellular function to favour differentiation instead of phenotype maintenance. The results raise questions as to the appropriateness of such STRs for stem cell expansion, and motivate better experimental studies to properly quantify the spatiotemporal variability in fluid shear stresses and its effect on stem cell expansion and stem cell fate.

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