Optimization of Ammonia Fiber Expansion (AFEX) Pretreatment and Enzymatic Hydrolysis of Miscanthus x giganteus to Fermentable Sugars

2007 ◽  
Vol 23 (4) ◽  
pp. 846-850 ◽  
Author(s):  
H.K. Murnen ◽  
V. Balan ◽  
S.P.S. Chundawat ◽  
B. Bals ◽  
L. daCostaSousa ◽  
...  
2007 ◽  
Vol 23 (4) ◽  
pp. 846-850 ◽  
Author(s):  
Hannah K. Murnen ◽  
Venkatesh Balan ◽  
Shishir P. S. Chundawat ◽  
Bryan Bals ◽  
Leonardo da Costa Sousa ◽  
...  

2021 ◽  
Author(s):  
Seyed Farshidreza Emam

Ammonia Fiber Expansion (AFEX) treatment is a technique that is able to enhance the enzymatic hydrolysis yield of lignocellulosic materials. In this technique, lignocellulosic materials are treated by liquid ammonia under pressure followed by rapid release of pressure that expands the fiber structure and increases enzyme access to lignocellulose polysaccharides. However, the AFEX treatment variables such as the mass ratio of ammonia to lignocellulosic biomass, moisture of lignocellulose (moisture content of biomass), temperature, and residence time need to be evaluated to find the maximum efficiency of this treatment. The efficiency of the AFEX pretreatment was quantified by the yield of released sugars during enzymatic hydrolysis of the AFEX-treated wheat straw. The optimal treatment conditions for wheat straw were found to be: ammonia-to-wheat straw ratio, 1:1; temperature, 95°C; moisture content of wheat straw, 70% (dry weight basis); and residence time, 5 minutes. Under these conditions, almost 89% of the theoretical sugars were released by enzymatic hydrolysis of the AFEX-treated wheat straw. The enzymatic hydrolysis results showed the significance of AFEX pretreatment of wheat straw when compared to untreated wheat straw with released sugars yield of only 26 %.


2021 ◽  
Author(s):  
Seyed Farshidreza Emam

Ammonia Fiber Expansion (AFEX) treatment is a technique that is able to enhance the enzymatic hydrolysis yield of lignocellulosic materials. In this technique, lignocellulosic materials are treated by liquid ammonia under pressure followed by rapid release of pressure that expands the fiber structure and increases enzyme access to lignocellulose polysaccharides. However, the AFEX treatment variables such as the mass ratio of ammonia to lignocellulosic biomass, moisture of lignocellulose (moisture content of biomass), temperature, and residence time need to be evaluated to find the maximum efficiency of this treatment. The efficiency of the AFEX pretreatment was quantified by the yield of released sugars during enzymatic hydrolysis of the AFEX-treated wheat straw. The optimal treatment conditions for wheat straw were found to be: ammonia-to-wheat straw ratio, 1:1; temperature, 95°C; moisture content of wheat straw, 70% (dry weight basis); and residence time, 5 minutes. Under these conditions, almost 89% of the theoretical sugars were released by enzymatic hydrolysis of the AFEX-treated wheat straw. The enzymatic hydrolysis results showed the significance of AFEX pretreatment of wheat straw when compared to untreated wheat straw with released sugars yield of only 26 %.


2013 ◽  
Vol 133 ◽  
pp. 307-314 ◽  
Author(s):  
Sujit Sadashiv Jagtap ◽  
Saurabh Sudha Dhiman ◽  
Tae-Su Kim ◽  
Jinglin Li ◽  
Jung-Kul Lee ◽  
...  

2013 ◽  
Vol 115 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Eloisa X. Leaes ◽  
Eduardo Zimmermann ◽  
Matheus Souza ◽  
Adriana P. Ramon ◽  
Ethiane T. Mezadri ◽  
...  

2021 ◽  
Vol 03 (03) ◽  
pp. 1-1
Author(s):  
Selvaraju Sivamani ◽  
◽  
Rajoo Baskar ◽  
Balasubramanian Lakshmi ◽  
◽  
...  

One of the crucial steps in the enzymatic hydrolysis of biomass is pretreatment. We investigated the applicability of a hybrid pretreatment method, This involved acid treatment followed by alkali before the production of fermentable sugars from cassava stem. The substrate was initially treated with acid (1% oxalic acid at 100 °C for 2 h), followed by treatment with a 10% NaOH–urea mixture (by mixing 5 g each of NaOH and urea in 100 mL of water) at 100 °C for 1 h. A cellulose recovery of 98.55% was obtained with the acid-alkali pretreatment method.


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